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Title: Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris

Abstract

A newly-recognized third fundamental mechanism of energy conservation in biology, electron bifurcation, uses free energy from exergonic redox reactions to drive endergonic redox reactions. Flavin-based electron bifurcation furnishes low potential electrons to demanding chemical reactions such as reduction of dinitrogen to ammonia. We employed the heterodimeric flavoenzyme FixAB from the diazotrophic bacterium Rhodopseudomonas palustris to elucidate unique properties that underpin flavin-based electron bifurcation.

Authors:
; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Energy Frontier Research Centers (EFRC) (United States). Center for Biological Electron Transfer and Catalysis (BETCy)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES); USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1769425
Alternate Identifier(s):
OSTI ID: 1422363
Report Number(s):
NREL/JA-2700-70488
Journal ID: ISSN 0021-9258; S0021925820397738; PII: S0021925820397738
Grant/Contract Number:  
SC0012518; AC36–08-GO28308; AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Name: Journal of Biological Chemistry Journal Volume: 293 Journal Issue: 13; Journal ID: ISSN 0021-9258
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; FixAB; reduction midpoint potential; electron bifurcation; electron transfer flavorprotein; ETF; flavorprotein; electron transfer; nitrogenase; flavin; circular dichroism

Citation Formats

Duan, H. Diessel, Lubner, Carolyn E., Tokmina-Lukaszewska, Monika, Gauss, George H., Bothner, Brian, King, Paul W., Peters, John W., and Miller, Anne-Frances. Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris. United States: N. p., 2018. Web. doi:10.1074/jbc.RA117.000707.
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Duan, H. Diessel, Lubner, Carolyn E., Tokmina-Lukaszewska, Monika, Gauss, George H., Bothner, Brian, King, Paul W., Peters, John W., & Miller, Anne-Frances. Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris. United States. https://doi.org/10.1074/jbc.RA117.000707
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Duan, H. Diessel, Lubner, Carolyn E., Tokmina-Lukaszewska, Monika, Gauss, George H., Bothner, Brian, King, Paul W., Peters, John W., and Miller, Anne-Frances. Thu . "Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris". United States. https://doi.org/10.1074/jbc.RA117.000707.
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@article{osti_1769425,
title = {Distinct properties underlie flavin-based electron bifurcation in a novel electron transfer flavoprotein FixAB from Rhodopseudomonas palustris},
author = {Duan, H. Diessel and Lubner, Carolyn E. and Tokmina-Lukaszewska, Monika and Gauss, George H. and Bothner, Brian and King, Paul W. and Peters, John W. and Miller, Anne-Frances},
abstractNote = {A newly-recognized third fundamental mechanism of energy conservation in biology, electron bifurcation, uses free energy from exergonic redox reactions to drive endergonic redox reactions. Flavin-based electron bifurcation furnishes low potential electrons to demanding chemical reactions such as reduction of dinitrogen to ammonia. We employed the heterodimeric flavoenzyme FixAB from the diazotrophic bacterium Rhodopseudomonas palustris to elucidate unique properties that underpin flavin-based electron bifurcation.},
doi = {10.1074/jbc.RA117.000707},
journal = {Journal of Biological Chemistry},
number = 13,
volume = 293,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 2018},
month = {Thu Mar 01 00:00:00 EST 2018}
}
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Journal Article:
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Publisher's Version of Record
https://doi.org/10.1074/jbc.RA117.000707
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Cited by: 15 works
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Figures / Tables:

Table 1 Table 1: Redox potentials of characterized ETFs measured at pH 7.0, or adjusted to pH 7.0.
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    One-megadalton metalloenzyme complex in Geobacter metallireducens involved in benzene ring reduction beyond the biological redox window
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    • Huwiler, Simona G.; Löffler, Claudia; Anselmann, Sebastian E. L.
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    • DOI: 10.1073/pnas.1819636116
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