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Title: Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels

Abstract

Here, the filamentous fungus Trichoderma reesei is found predominantly in the tropics but also in more temperate regions, such as Europe, and is widely known as a producer of large amounts of plant cell wall-degrading enzymes. We sequenced the genome of the sexually competent isolate CBS999.97, which is phenotypically different from the female sterile strain QM6a but can cross sexually with QM6a. Transcriptome data for growth on cellulose showed that entire carbohydrate-active enzyme (CAZyme) families are consistently differentially regulated between these strains. We evaluated backcrossed strains of both mating types, which acquired female fertility from CBS999.97 but maintained a mostly QM6a genetic background, and we could thereby distinguish between the effects of strain background and female fertility or mating type. We found clear regulatory differences associated with female fertility and female sterility, including regulation of CAZyme and transporter genes. Analysis of carbon source utilization, transcriptomes, and secondary metabolites in these strains revealed that only a few changes in gene regulation are consistently correlated with different mating types. Different strain backgrounds (QM6a versus CBS999.97) resulted in the most significant alterations in the transcriptomes and in carbon source utilization, with decreased growth of CBS999.97 on several amino acids (for example proline ormore » alanine), which further correlated with the downregulation of genes involved in the respective pathways. In combination, our findings support a role of fertility-associated processes in physiology and gene regulation and are of high relevance for the use of sexual crossing in combining the characteristics of two compatible strains or quantitative trait locus (QTL) analysis.« less

Authors:
 [1];  [2];  [3];  [4];  [3];  [5];  [5];  [5];  [1];  [6];  [6];  [7];  [8];  [8];  [5]; ORCiD logo [9];  [10]
  1. TU Wien, Vienna (Austria)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Oregon State Univ., Corvallis, OR (United States)
  3. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  4. Oregon State Univ., Corvallis, OR (United States)
  5. Academia Sinica, Taipei (Taiwan)
  6. AIT Austrian Institute of Technology, Tulln (Austria)
  7. Univ. of Natural Resources and Life Sciences BOKU, Tulln (Austria)
  8. Univ. of Natural Resources and Life Sciences Vienna, Tulln (Austria)
  9. TU Wien, Vienna (Austria); AIT Austrian Institute of Technology, Tulln (Austria)
  10. Univ. of Bayreuth, Bayreuth (Germany)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1419919
Report Number(s):
PNNL-SA-129552
Journal ID: ISSN 0099-2240; KP1704020
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 83; Journal Issue: 22; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Trichoderma reesei; Hypocrea jecorina; cellulase; strain improvement; sexual development; female fertility; secondary metabolism; mating type; carbon source

Citation Formats

Tisch, Doris, Pomraning, Kyle R., Collett, James R., Freitag, Michael, Baker, Scott E., Chen, Chia -Ling, Hsu, Paul Wei-Che, Chuang, Yu Chien, Schuster, Andre, Dattenbock, Christoph, Stappler, Eva, Sulyok, Michael, Bohmdorfer, Stefan, Oberlerchner, Josua, Wang, Ting -Fang, Schmoll, Monika, and Drake, Harold L. Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels. United States: N. p., 2017. Web. doi:10.1128/AEM.01578-17.
Tisch, Doris, Pomraning, Kyle R., Collett, James R., Freitag, Michael, Baker, Scott E., Chen, Chia -Ling, Hsu, Paul Wei-Che, Chuang, Yu Chien, Schuster, Andre, Dattenbock, Christoph, Stappler, Eva, Sulyok, Michael, Bohmdorfer, Stefan, Oberlerchner, Josua, Wang, Ting -Fang, Schmoll, Monika, & Drake, Harold L. Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels. United States. doi:10.1128/AEM.01578-17.
Tisch, Doris, Pomraning, Kyle R., Collett, James R., Freitag, Michael, Baker, Scott E., Chen, Chia -Ling, Hsu, Paul Wei-Che, Chuang, Yu Chien, Schuster, Andre, Dattenbock, Christoph, Stappler, Eva, Sulyok, Michael, Bohmdorfer, Stefan, Oberlerchner, Josua, Wang, Ting -Fang, Schmoll, Monika, and Drake, Harold L. Fri . "Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels". United States. doi:10.1128/AEM.01578-17. https://www.osti.gov/servlets/purl/1419919.
@article{osti_1419919,
title = {Omics Analyses of Trichoderma reesei CBS999.97 and QM6a Indicate the Relevance of Female Fertility to Carbohydrate-Active Enzyme and Transporter Levels},
author = {Tisch, Doris and Pomraning, Kyle R. and Collett, James R. and Freitag, Michael and Baker, Scott E. and Chen, Chia -Ling and Hsu, Paul Wei-Che and Chuang, Yu Chien and Schuster, Andre and Dattenbock, Christoph and Stappler, Eva and Sulyok, Michael and Bohmdorfer, Stefan and Oberlerchner, Josua and Wang, Ting -Fang and Schmoll, Monika and Drake, Harold L.},
abstractNote = {Here, the filamentous fungus Trichoderma reesei is found predominantly in the tropics but also in more temperate regions, such as Europe, and is widely known as a producer of large amounts of plant cell wall-degrading enzymes. We sequenced the genome of the sexually competent isolate CBS999.97, which is phenotypically different from the female sterile strain QM6a but can cross sexually with QM6a. Transcriptome data for growth on cellulose showed that entire carbohydrate-active enzyme (CAZyme) families are consistently differentially regulated between these strains. We evaluated backcrossed strains of both mating types, which acquired female fertility from CBS999.97 but maintained a mostly QM6a genetic background, and we could thereby distinguish between the effects of strain background and female fertility or mating type. We found clear regulatory differences associated with female fertility and female sterility, including regulation of CAZyme and transporter genes. Analysis of carbon source utilization, transcriptomes, and secondary metabolites in these strains revealed that only a few changes in gene regulation are consistently correlated with different mating types. Different strain backgrounds (QM6a versus CBS999.97) resulted in the most significant alterations in the transcriptomes and in carbon source utilization, with decreased growth of CBS999.97 on several amino acids (for example proline or alanine), which further correlated with the downregulation of genes involved in the respective pathways. In combination, our findings support a role of fertility-associated processes in physiology and gene regulation and are of high relevance for the use of sexual crossing in combining the characteristics of two compatible strains or quantitative trait locus (QTL) analysis.},
doi = {10.1128/AEM.01578-17},
journal = {Applied and Environmental Microbiology},
number = 22,
volume = 83,
place = {United States},
year = {2017},
month = {9}
}

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