Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Awel, Salah; Kirian, Richard A.; Wiedorn, Max O.; Beyerlein, Kenneth R.; Roth, Nils; Horke, Daniel A.; Oberthür, Dominik; Knoska, Juraj; Mariani, Valerio; Morgan, Andrew; Adriano, Luigi; Tolstikova, Alexandra; Xavier, P. Lourdu; Yefanov, Oleksandr; Aquila, Andrew; Barty, Anton; Roy-Chowdhury, Shatabdi; Hunter, Mark S.; James, Daniel; Robinson, Joseph S.; Weierstall, Uwe; Rode, Andrei V.; Bajt, Saša; Küpper, Jochen; Chapman, Henry N.

doi:10.1107/S1600576717018131

Title: Femtosecond X-ray diffraction from an aerosolized beam of protein nanocrystals

Journal Article · Thu Feb 01 00:00:00 UTC 2018 · Journal of Applied Crystallography (Online)

DOI: https://doi.org/10.1107/S1600576717018131 · OSTI ID:1418182

; Kirian, Richard A.;

; Beyerlein, Kenneth R.; Roth, Nils; Horke, Daniel A.; Oberthür, Dominik; Knoska, Juraj; Mariani, Valerio; Morgan, Andrew; Adriano, Luigi; Tolstikova, Alexandra;

; Yefanov, Oleksandr; Aquila, Andrew; Barty, Anton; Roy-Chowdhury, Shatabdi; Hunter, Mark S.; James, Daniel; Robinson, Joseph S. more »

High-resolution Bragg diffraction from aerosolized single granulovirus nanocrystals using an X-ray free-electron laser is demonstrated. The outer dimensions of the in-vacuum aerosol injector components are identical to conventional liquid-microjet nozzles used in serial diffraction experiments, which allows the injector to be utilized with standard mountings. As compared with liquid-jet injection, the X-ray scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. Such reduction is required for diffraction measurements of small macromolecular nanocrystals and single particles. High particle speeds are achieved, making the approach suitable for use at upcoming high-repetition-rate facilities.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: European Union (EU); German Research Foundation (DFG); National Institutes of Health (NIH); National Science Foundation (NSF); USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1418182

Journal Information:: Journal of Applied Crystallography (Online), Journal Name: Journal of Applied Crystallography (Online) Journal Issue: 1 Vol. 51; ISSN JACGAR; ISSN 1600-5767

Publisher:: International Union of Crystallography (IUCr)Copyright Statement

Country of Publication:: Denmark

Language:: English

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