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Title: Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I

Curcumin is a polyphenolic phytonutrient that has antineurodegenerative properties. Here, we investigated the anti-amyloidogenic properties of curcumin. Following incubation with curcumin, intrinsic tryptophan fluorescence emission of apolipoprotein (apo) A-I was strongly quenched. At the same time, curcumin fluorescence emission was enhanced. The fluorescence emission spectra of curcumin in the presence of amyloid-like aggregates formed by methionine-oxidized (ox) apoA-I varied, depending on whether curcumin was added before, or after, aggregate formation. The impact of curcumin on the structure of the aggregating material was revealed by the lower amount of β-structure in ox-apoA-I amyloid-like aggregates formed in the presence of curcumin, compared to aggregates formed without curcumin. However, the kinetics of ox-apoA-I amyloid-like aggregate formation was not altered by the presence of curcumin. Moreover, electron microscopy analysis detected no discernable differences in amyloid morphology when ox-apoA-I amyloid-like aggregates were formed in the presence or absence of curcumin. In conclusion, curcumin interacts with apoA-I and alters the structure of ox-apoA-I amyloid-like aggregates yet does not diminish the propensity of ox-apoA-I to form aggregates.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4] ;  [3] ;  [5]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Nutritional Sciences and Toxicology
  2. Children's Hospital Oakland Research Inst., Oakland, CA (United States); Univ. of Tehran (Iran). Dept. of Biotechnology and College of Science
  3. Children's Hospital Oakland Research Inst., Oakland, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). The Molecular Foundry
  5. Univ. of California, Berkeley, CA (United States). Dept. of Nutritional Sciences and Toxicology; Children's Hospital Oakland Research Inst., Oakland, CA (United States); Univ. of Nevada, Reno, NV (United States). Dept. of Biochemistry and Molecular Biology
Publication Date:
Grant/Contract Number:
AC02-05CH11231; R37‐HL64159; R01‐HL113059; HL115153; GM104427
Type:
Published Article
Journal Name:
FEBS Open Bio
Additional Journal Information:
Journal Volume: 8; Journal Issue: 2; Related Information: © 2017 The Authors. Published by FEBS Press and John Wiley & Sons Ltd.; Journal ID: ISSN 2211-5463
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; amyloid‐like aggregate; Apolipoprotein A‐I; curcumin; electron microscopy; fluorescence spectroscopy; methionine oxidation
OSTI Identifier:
1416461
Alternate Identifier(s):
OSTI ID: 1416605; OSTI ID: 1433116