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Title: Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I

Abstract

Curcumin is a polyphenolic phytonutrient that has antineurodegenerative properties. Here, we investigated the anti-amyloidogenic properties of curcumin. Following incubation with curcumin, intrinsic tryptophan fluorescence emission of apolipoprotein (apo) A-I was strongly quenched. At the same time, curcumin fluorescence emission was enhanced. The fluorescence emission spectra of curcumin in the presence of amyloid-like aggregates formed by methionine-oxidized (ox) apoA-I varied, depending on whether curcumin was added before, or after, aggregate formation. The impact of curcumin on the structure of the aggregating material was revealed by the lower amount of β-structure in ox-apoA-I amyloid-like aggregates formed in the presence of curcumin, compared to aggregates formed without curcumin. However, the kinetics of ox-apoA-I amyloid-like aggregate formation was not altered by the presence of curcumin. Moreover, electron microscopy analysis detected no discernable differences in amyloid morphology when ox-apoA-I amyloid-like aggregates were formed in the presence or absence of curcumin. In conclusion, curcumin interacts with apoA-I and alters the structure of ox-apoA-I amyloid-like aggregates yet does not diminish the propensity of ox-apoA-I to form aggregates.

Authors:
 [1];  [2];  [3];  [4];  [4];  [3];  [5]
  1. Univ. of California, Berkeley, CA (United States). Dept. of Nutritional Sciences and Toxicology
  2. Children's Hospital Oakland Research Inst., Oakland, CA (United States); Univ. of Tehran (Iran). Dept. of Biotechnology and College of Science
  3. Children's Hospital Oakland Research Inst., Oakland, CA (United States)
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). The Molecular Foundry
  5. Univ. of California, Berkeley, CA (United States). Dept. of Nutritional Sciences and Toxicology; Children's Hospital Oakland Research Inst., Oakland, CA (United States); Univ. of Nevada, Reno, NV (United States). Dept. of Biochemistry and Molecular Biology
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Institutes of Health (NIH)
OSTI Identifier:
1416461
Alternate Identifier(s):
OSTI ID: 1416605; OSTI ID: 1433116
Grant/Contract Number:  
AC02-05CH11231; R37‐HL64159; R01‐HL113059; HL115153; GM104427
Resource Type:
Published Article
Journal Name:
FEBS Open Bio
Additional Journal Information:
Journal Name: FEBS Open Bio Journal Volume: 8 Journal Issue: 2; Journal ID: ISSN 2211-5463
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
Country unknown/Code not available
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; amyloid‐like aggregate; Apolipoprotein A‐I; curcumin; electron microscopy; fluorescence spectroscopy; methionine oxidation

Citation Formats

Krishnamoorthy, Aparna, Tavoosi, Narjes, Chan, Gary K. L., Liu, Jianfang, Ren, Gang, Cavigiolio, Giorgio, and Ryan, Robert O. Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I. Country unknown/Code not available: N. p., 2018. Web. doi:10.1002/2211-5463.12372.
Krishnamoorthy, Aparna, Tavoosi, Narjes, Chan, Gary K. L., Liu, Jianfang, Ren, Gang, Cavigiolio, Giorgio, & Ryan, Robert O. Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I. Country unknown/Code not available. doi:10.1002/2211-5463.12372.
Krishnamoorthy, Aparna, Tavoosi, Narjes, Chan, Gary K. L., Liu, Jianfang, Ren, Gang, Cavigiolio, Giorgio, and Ryan, Robert O. Wed . "Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I". Country unknown/Code not available. doi:10.1002/2211-5463.12372.
@article{osti_1416461,
title = {Effect of curcumin on amyloid-like aggregates generated from methionine-oxidized apolipoprotein A-I},
author = {Krishnamoorthy, Aparna and Tavoosi, Narjes and Chan, Gary K. L. and Liu, Jianfang and Ren, Gang and Cavigiolio, Giorgio and Ryan, Robert O.},
abstractNote = {Curcumin is a polyphenolic phytonutrient that has antineurodegenerative properties. Here, we investigated the anti-amyloidogenic properties of curcumin. Following incubation with curcumin, intrinsic tryptophan fluorescence emission of apolipoprotein (apo) A-I was strongly quenched. At the same time, curcumin fluorescence emission was enhanced. The fluorescence emission spectra of curcumin in the presence of amyloid-like aggregates formed by methionine-oxidized (ox) apoA-I varied, depending on whether curcumin was added before, or after, aggregate formation. The impact of curcumin on the structure of the aggregating material was revealed by the lower amount of β-structure in ox-apoA-I amyloid-like aggregates formed in the presence of curcumin, compared to aggregates formed without curcumin. However, the kinetics of ox-apoA-I amyloid-like aggregate formation was not altered by the presence of curcumin. Moreover, electron microscopy analysis detected no discernable differences in amyloid morphology when ox-apoA-I amyloid-like aggregates were formed in the presence or absence of curcumin. In conclusion, curcumin interacts with apoA-I and alters the structure of ox-apoA-I amyloid-like aggregates yet does not diminish the propensity of ox-apoA-I to form aggregates.},
doi = {10.1002/2211-5463.12372},
journal = {FEBS Open Bio},
number = 2,
volume = 8,
place = {Country unknown/Code not available},
year = {2018},
month = {1}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: 10.1002/2211-5463.12372

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Cited by: 1 work
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