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Title: The Kalanchoe genome provides insights into convergent evolution and building blocks of crassulacean acid metabolism

Crassulacean acid metabolism (CAM) is a specialized photosynthetic adaptation to arid environments, found predominantly in diverse eudicotyledonous (eudicot) and monocotyledonous (monocot) lineages that diverged approximately 135 million years ago. To test whether convergent evolution underpins the independent emergences of CAM, we present de novo genome assembly and gene expression data for Kalanchoë fedtschenkoi, an obligate CAM species that was shown by multigene phylogenetic analysis to represent one of the earliest-diverging lineages of core eudicots. Our combined analysis of K. fedtschenkoi and two monocot CAM species (Ananas comosus and Phalaenopsis equestris) identified signatures of convergence in protein sequence and in the diel re-scheduling of genes involved in metabolism and signaling. Lastly, our results provide significant insight into CAM evolution, facilitating CAM-into-C 3 engineering for enhancing drought tolerance in crops.
Authors:
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  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States)
  4. USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
  5. Fujian Agriculture and Forestry Univ., Fujian (China)
  6. Univ. of Nevada, Reno, NV (United States)
  7. Univ. of Georgia, Athens, GA (United States)
  8. Univ. of Tennessee, Knoxville, TN (United States)
  9. Northern Illinois Univ., DeKalb, IL (United States)
  10. Univ. of Liverpool, Liverpool (United Kingdom)
  11. Univ. of Oxford, Oxford (United Kingdom)
  12. Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  13. Pacific Biosciences, Inc., Menlo Park, CA (United States)
  14. Michigan State Univ., East Lansing, MI (United States)
  15. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Newcastle Univ., Newcastle upon Tyne (United Kingdom)
  16. Fujian Agriculture and Forestry Univ., Fujian (China); Univ. of Illinois at Urbana-Champaign, Urbana, IL (United States)
  17. Smithsonian Tropical Research Institute, Ancon (Republic of Panama)
  18. HudsonAlpha Institute for Biotechnology, Huntsville, AL (United States); USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Publication Date:
Grant/Contract Number:
AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 8; Journal Issue: 1; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
OSTI Identifier:
1413629