Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids
Abstract
Summary Flavonoids ubiquitously distribute to the terrestrial plants and chalcone isomerase ( CHI )‐catalyzed intramolecular and stereospecific cyclization of chalcones is a committed step in the production of flavonoids. However, so far the bona fide CHI s are found only in vascular plants, and their origin and evolution remains elusive. We conducted transcriptomic and/or genomic sequence search, subsequent phylogenetic analysis, and detailed biochemical and genetic characterization to explore the potential existence of CHI proteins in the basal bryophyte liverwort species and the lycophyte Selaginella moellendorffii . We found that both liverwort and Selaginella species possess canonical CHI ‐fold proteins that cluster with their corresponding higher plant counterparts. Among them, some members exhibited bona fide CHI activity, which catalyze stereospecific cyclization of both 6′‐hydroxychalcone and 6′‐deoxychalcone, yielding corresponding 5‐hydroxy and 5‐deoxyflavanones, resembling the typical type II CHI s currently known to be ‘specific’ for legume plants. Expressing those primitive bona fide CHI s in the Arabidopsis chi mutant restores the seed coat transparent testa phenotype and the accumulation of flavonoids. These findings, in contrast to our current understanding of the evolution of enzymatic CHI s, suggest that emergence of the bona fide type II CHI s is an ancient evolution eventmore »
- Authors:
-
[2];
- Shandong Univ., Jinan (China). Key Lab. of Chemical Biology of Natural Products, Ministry of Education, School of Pharmaceutical Sciences
- Brookhaven National Lab. (BNL), Upton, NY (United States). Biology Dept.
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1412668
- Alternate Identifier(s):
- OSTI ID: 1405542
- Report Number(s):
- BNL-114460-2017-JA
Journal ID: ISSN 0028-646X
- Grant/Contract Number:
- SC0012704; DE‐SC0012704; MCB-1051675
- Resource Type:
- Accepted Manuscript
- Journal Name:
- New Phytologist
- Additional Journal Information:
- Journal Volume: 217; Journal Issue: 2; Journal ID: ISSN 0028-646X
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Flavonoids; Chalcone isomerase; Evolution; Liverwort; Selaginella moellendorffii (Spikemoss)
Citation Formats
Cheng, Ai-Xia, Zhang, Xuebin, Han, Xiao-Juan, Zhang, Yu-Ying, Gao, Shuai, Liu, Chang-Jun, and Lou, Hong-Xiang. Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids. United States: N. p., 2017.
Web. doi:10.1111/nph.14852.
Cheng, Ai-Xia, Zhang, Xuebin, Han, Xiao-Juan, Zhang, Yu-Ying, Gao, Shuai, Liu, Chang-Jun, & Lou, Hong-Xiang. Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids. United States. https://doi.org/10.1111/nph.14852
Cheng, Ai-Xia, Zhang, Xuebin, Han, Xiao-Juan, Zhang, Yu-Ying, Gao, Shuai, Liu, Chang-Jun, and Lou, Hong-Xiang. Mon .
"Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids". United States. https://doi.org/10.1111/nph.14852. https://www.osti.gov/servlets/purl/1412668.
@article{osti_1412668,
title = {Identification of chalcone isomerase in the basal land plants reveals an ancient evolution of enzymatic cyclization activity for synthesis of flavonoids},
author = {Cheng, Ai-Xia and Zhang, Xuebin and Han, Xiao-Juan and Zhang, Yu-Ying and Gao, Shuai and Liu, Chang-Jun and Lou, Hong-Xiang},
abstractNote = {Summary Flavonoids ubiquitously distribute to the terrestrial plants and chalcone isomerase ( CHI )‐catalyzed intramolecular and stereospecific cyclization of chalcones is a committed step in the production of flavonoids. However, so far the bona fide CHI s are found only in vascular plants, and their origin and evolution remains elusive. We conducted transcriptomic and/or genomic sequence search, subsequent phylogenetic analysis, and detailed biochemical and genetic characterization to explore the potential existence of CHI proteins in the basal bryophyte liverwort species and the lycophyte Selaginella moellendorffii . We found that both liverwort and Selaginella species possess canonical CHI ‐fold proteins that cluster with their corresponding higher plant counterparts. Among them, some members exhibited bona fide CHI activity, which catalyze stereospecific cyclization of both 6′‐hydroxychalcone and 6′‐deoxychalcone, yielding corresponding 5‐hydroxy and 5‐deoxyflavanones, resembling the typical type II CHI s currently known to be ‘specific’ for legume plants. Expressing those primitive bona fide CHI s in the Arabidopsis chi mutant restores the seed coat transparent testa phenotype and the accumulation of flavonoids. These findings, in contrast to our current understanding of the evolution of enzymatic CHI s, suggest that emergence of the bona fide type II CHI s is an ancient evolution event that occurred before the divergence of liverwort lineages.},
doi = {10.1111/nph.14852},
journal = {New Phytologist},
number = 2,
volume = 217,
place = {United States},
year = {2017},
month = {10}
}
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