Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Azadmanesh, Jahaun; Trickel, Scott R.; Weiss, Kevin L.; Coates, Leighton; Borgstahl, Gloria E.  O.

doi:10.1107/S2053230X17003508

Title: Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

Journal Article · 28 March 2017 · Acta Crystallographica. Section F, Structural Biology Communications

DOI: https://doi.org/10.1107/S2053230X17003508 · OSTI ID:1407982

Azadmanesh, Jahaun ^[1]; Trickel, Scott R. ^[2];

^[3];

^[3]; Borgstahl, Gloria E. O. ^[1]

Nebraska Medical Center, Omaha, NE (United States). Eppley Inst. for Research in Cancer and Allied Diseases, Dept. of Biochemistry and Molecular Biology
Nebraska Medical Center, Omaha, NE (United States). Eppley Inst. for Research in Cancer and Allied Diseases
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Biology and Soft Matter Division

Superoxide dismutases (SODs) are enzymes that protect against oxidative stress by dismutation of superoxide into oxygen and hydrogen peroxide through cyclic reduction and oxidation of the active-site metal. The complete enzymatic mechanisms of SODs are unknown since data on the positions of hydrogen are limited. Here, methods are presented for large crystal growth and neutron data collection of human manganese SOD (MnSOD) using perdeuteration and the MaNDi beamline at Oak Ridge National Laboratory. The crystal from which the human MnSOD data set was obtained is the crystal with the largest unit-cell edge (240 Å) from which data have been collectedvianeutron diffraction to sufficient resolution (2.30 Å) where hydrogen positions can be observed.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); National Aeronautics and Space Administration (NASA); National Institutes of Health (NIH)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1407982

Alternate ID(s):: OSTI ID: 1437709

Journal Information:: Acta Crystallographica. Section F, Structural Biology Communications, Journal Name: Acta Crystallographica. Section F, Structural Biology Communications Journal Issue: 4 Vol. 73; ISSN ACSFEN; ISSN 2053-230X

Publisher:: International Union of CrystallographyCopyright Statement

Country of Publication:: United States

Language:: English

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Improving the accuracy and resolution of neutron crystallographic data by three-dimensional profile fitting of Bragg peaks in reciprocal space Sullivan, Brendan; Archibald, Rick; Langan, Patricia S. Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 11 https://doi.org/10.1107/s2059798318013347	journal	October 2018
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Redox manipulation of the manganese metal in human manganese superoxide dismutase for neutron diffraction Azadmanesh, Jahaun; Lutz, William E.; Weiss, Kevin L. Acta Crystallographica Section F Structural Biology Communications, Vol. 74, Issue 10 https://doi.org/10.1107/s2053230x18011299	journal	September 2018
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37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
60 APPLIED LIFE SCIENCES
manganese superoxide dismutase
neutron diffraction
perdeuteration
human
large unit cell

Title: Preliminary neutron diffraction analysis of challenging human manganese superoxide dismutase crystals

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