Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering

Farshid, Behzad; Lalwani, Gaurav; Shir Mohammadi, Meisam; Simonsen, John; Sitharaman, Balaji

doi:10.1002/jbm.b.33565

Title: Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering

Abstract

Abstract This study investigates the mechanical properties and in vitro cytotoxicity of one‐ and two‐dimensional boron nitride nanomaterials‐reinforced biodegradable polymeric nanocomposites. Poly(propylene fumarate) (PPF) nanocomposites were fabricated using crosslinking agent N ‐vinyl pyrrolidone and inorganic nanomaterials: boron nitride nanotubes (BNNTs) and boron nitride nanoplatelets (BNNPs) dispersed at 0.2 wt % in the polymeric matrix. The incorporation of BNNPs and BNNTs resulted in a ∼38 and ∼15% increase in compressive (Young's) modulus, and ∼31 and ∼6% increase in compressive yield strength compared to PPF control, respectively. The nanocomposites showed a time‐dependent increased protein adsorption for collagen I protein. The cytotoxicity evaluation of aqueous BNNT and BNNP dispersions (at 1–100 μg/mL concentrations) using murine MC3T3 preosteoblast cells showed ∼73–99% viability. The cytotoxicity evaluation of media extracts of nanocomposites before crosslinking, after crosslinking, and upon degradation (using 1×–100× dilutions) showed dose‐dependent cytotoxicity responses. Crosslinked nanocomposites showed excellent (∼79–100%) cell viability, cellular attachment (∼57–67%), and spreading similar to cells grown on the surface of tissue culture polystyrene control. The media extracts of degradation products showed a dose‐dependent cytotoxicity. The favorable cytocompatibility results in combination with improved mechanical properties of BNNT and BNNP nanocomposites opens new avenues for further in vitro and in vivo safetymore »« less

Authors:

Farshid, Behzad ^[1];

^[2]; Shir Mohammadi, Meisam ^[3]; Simonsen, John ^[3];

^[2]

Department of Biomedical Engineering Stony Brook University Stony Brook New York 11794, Department of Materials Science and Engineering Stony Brook University Stony Brook New York 11794
Department of Biomedical Engineering Stony Brook University Stony Brook New York 11794
Department of Wood Science and Engineering Oregon State University Corvallis Oregon 97331

Publication Date:: Tue Nov 03 00:00:00 EST 2015

Sponsoring Org.:: USDOE

OSTI Identifier:: 1401638

Grant/Contract Number:: DE‐AC02‐98CH10886

Resource Type:: Publisher's Accepted Manuscript

Journal Name:: Journal of Biomedical Materials Research - Part B: Applied Biomaterials

Additional Journal Information:: Journal Name: Journal of Biomedical Materials Research - Part B: Applied Biomaterials Journal Volume: 105 Journal Issue: 2; Journal ID: ISSN 1552-4973

Publisher:: Wiley Blackwell (John Wiley & Sons)

Country of Publication:: United States

Language:: English

Citation Formats


                    Farshid, Behzad, Lalwani, Gaurav, Shir Mohammadi, Meisam, Simonsen, John, and Sitharaman, Balaji. Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering.  United States: N. p., 2015. 
Web.  doi:10.1002/jbm.b.33565.

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                    Farshid, Behzad, Lalwani, Gaurav, Shir Mohammadi, Meisam, Simonsen, John, & Sitharaman, Balaji. Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering.  United States.  https://doi.org/10.1002/jbm.b.33565

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                    Farshid, Behzad, Lalwani, Gaurav, Shir Mohammadi, Meisam, Simonsen, John, and Sitharaman, Balaji. Tue .  
"Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering".  United States.  https://doi.org/10.1002/jbm.b.33565.

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@article{osti_1401638,

  title        = {Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering},

  author       = {Farshid, Behzad and Lalwani, Gaurav and Shir Mohammadi, Meisam and Simonsen, John and Sitharaman, Balaji},

  abstractNote = {Abstract This study investigates the mechanical properties and in vitro cytotoxicity of one‐ and two‐dimensional boron nitride nanomaterials‐reinforced biodegradable polymeric nanocomposites. Poly(propylene fumarate) (PPF) nanocomposites were fabricated using crosslinking agent N ‐vinyl pyrrolidone and inorganic nanomaterials: boron nitride nanotubes (BNNTs) and boron nitride nanoplatelets (BNNPs) dispersed at 0.2 wt % in the polymeric matrix. The incorporation of BNNPs and BNNTs resulted in a ∼38 and ∼15% increase in compressive (Young's) modulus, and ∼31 and ∼6% increase in compressive yield strength compared to PPF control, respectively. The nanocomposites showed a time‐dependent increased protein adsorption for collagen I protein. The cytotoxicity evaluation of aqueous BNNT and BNNP dispersions (at 1–100 μg/mL concentrations) using murine MC3T3 preosteoblast cells showed ∼73–99% viability. The cytotoxicity evaluation of media extracts of nanocomposites before crosslinking, after crosslinking, and upon degradation (using 1×–100× dilutions) showed dose‐dependent cytotoxicity responses. Crosslinked nanocomposites showed excellent (∼79–100%) cell viability, cellular attachment (∼57–67%), and spreading similar to cells grown on the surface of tissue culture polystyrene control. The media extracts of degradation products showed a dose‐dependent cytotoxicity. The favorable cytocompatibility results in combination with improved mechanical properties of BNNT and BNNP nanocomposites opens new avenues for further in vitro and in vivo safety and efficacy studies towards bone tissue engineering applications. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 406–419, 2017.},

  doi          = {10.1002/jbm.b.33565},

  journal      = {Journal of Biomedical Materials Research - Part B: Applied Biomaterials},

  number       = 2,

  volume       = 105,

  place        = {United States},

  year         = {Tue Nov 03 00:00:00 EST 2015},

  month        = {Tue Nov 03 00:00:00 EST 2015}

}

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Title: Boron nitride nanotubes and nanoplatelets as reinforcing agents of polymeric matrices for bone tissue engineering

Abstract

Citation Formats

Surface Roughness, Porosity, and Texture as Modifiers of Cellular Adhesion journal, December 1996

Boron nitride nanotube reinforced polylactide–polycaprolactone copolymer composite: Mechanical properties and cytocompatibility with osteoblasts and macrophages in vitro journal, September 2010

Assembly of Graphene Sheets into 3D Macroscopic Structures journal, May 2012

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Injectable Nanocomposites of Single-Walled Carbon Nanotubes and Biodegradable Polymers for Bone Tissue Engineering journal, July 2006

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Degradation and biocompatibility of a poly(propylene fumarate)-based/alumoxane nanocomposite for bone tissue engineering journal, January 2007

Porous boron nitride nanosheets for effective water cleaning journal, April 2013

In vitro degradation of a poly(propylene fumarate)-based composite material journal, November 1996

Synthesis, characterization, in vitro phantom imaging, and cytotoxicity of a novel graphene-based multimodal magnetic resonance imaging-X-ray computed tomography contrast agent journal, January 2014

Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells journal, June 2015

Soluble, Exfoliated Hexagonal Boron Nitride Nanosheets journal, November 2009

In Vitro Cytotoxicity of Injectable and Biodegradable Poly(propylene fumarate)-Based Networks: Unreacted Macromers, Cross-Linked Networks, and Degradation Products journal, July 2003

Recent progress in the research of inorganic fullerene-like nanoparticles and inorganic nanotubes journal, January 2010

Young modulus, mechanical and electrical properties of isolated individual and bundled single-walled boron nitride nanotubes journal, May 2011

Isolation and Characterization of MC3T3-E1 Preosteoblast Subclones with Distinct In Vitro and In Vivo Differentiation/Mineralization Potential journal, June 1999

Polymer composites of boron nitride nanotubes and nanosheets journal, January 2014

Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells journal, January 2014

Catalyzed Collapse and Enhanced Hydrogen Storage of BN Nanotubes journal, December 2002

Injectable in situ cross-linkable nanocomposites of biodegradable polymers and carbon nanostructures for bone tissue engineering journal, January 2007

In vitro cytotoxicity of single‐walled carbon nanotube/biodegradable polymer nanocomposites journal, September 2008

Thermal Diffusivity of Hexagonal Boron Nitride Composites Based on Cross-Linked Liquid Crystalline Polyimides journal, January 2013

Fibrin(ogen) mediates acute inflammatory responses to biomaterials journal, December 1993

Boron nitride nanotubes: functionalization and composites journal, January 2008

Insight into reactions and interface between boron nitride nanotube and aluminum journal, September 2012

Graphene-based contrast agents for photoacoustic and thermoacoustic tomography journal, December 2013

Mechanical and electronic properties of carbon and boron–nitride nanotubes journal, January 2000

Fabrication and characterization of three-dimensional macroscopic all-carbon scaffolds journal, March 2013

In Vitro Investigation of the Cellular Toxicity of Boron Nitride Nanotubes journal, April 2011

The effects of graphene nanostructures on mesenchymal stem cells journal, June 2014

Promises, facts and challenges for carbon nanotubes in imaging and therapeutics journal, September 2009

Synthesis of poly(propylene fumarate) journal, April 2009

A review of particulate reinforcement theories for polymer composites journal, December 1990

In Vitro Degradation of a Poly(Propylene Fumarate)/ β -Tricalcium Phosphate Composite Orthopaedic Scaffold journal, June 1997

Role of the benzoyloxyl radical in DNA damage mediated by benzoyl peroxide journal, March 1991

In vitro cytocompatibility of one-dimensional and two-dimensional nanostructure-reinforced biodegradable polymeric nanocomposites journal, November 2014

Boron nitride nanotube reinforced hydroxyapatite composite: Mechanical and tribological performance and in-vitro biocompatibility to osteoblasts journal, January 2011

Two-Dimensional Nanostructure-Reinforced Biodegradable Polymeric Nanocomposites for Bone Tissue Engineering journal, February 2013

Nanoreinforcement of Poly(propylene fumarate)-Based Networks with Surface Modified Alumoxane Nanoparticles for Bone Tissue Engineering journal, September 2004

Fibronectin-integrin interactions journal, January 1997

The Bicinchoninic Acid (BCA) Assay for Protein Quantitation book, January 2009

Two-dimensional graphene analogues for biomedical applications journal, January 2015

Enhancement of Protein Adsorption Induced by Surface Roughness journal, December 2006

Preparation of poly(vinyl alcohol) nanocomposites with molybdenum disulfide (MoS2): structural characteristics and markedly enhanced properties journal, January 2012

The reliability and limits of the MTT reduction assay for carbon nanotubes–cell interaction journal, November 2007

Adsorption of BSA on Highly Carboxylated Microspheres—Quantitative Effects of Surface Functional Groups and Interaction Forces journal, February 1996

Measurement of protein adsorption kinetics by an in situ, “real-time,” solution depletion technique journal, April 1992

Large Scale Growth and Characterization of Atomic Hexagonal Boron Nitride Layers journal, August 2010

Comparative theoretical study of single-wall carbon and boron-nitride nanotubes journal, June 2003

Tungsten disulfide nanotubes reinforced biodegradable polymers for bone tissue engineering journal, September 2013

Properties of Boron Nitride Nanotubes conference, January 2003

A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity journal, November 1988

Nanotube composites journal, June 2007

Bone-Tissue-Engineering Material Poly(propylene fumarate): Correlation between Molecular Weight, Chain Dimensions, and Physical Properties journal, June 2006

The effect of particle shape on the mechanical properties of filled polymers journal, August 1980

Fabrication of Alumina Ceramic Reinforced with Boron Nitride Nanotubes with Improved Mechanical Properties journal, September 2011

Surface Roughness, Porosity, and Texture as Modifiers of Cellular Adhesion
journal, December 1996

Boron nitride nanotube reinforced polylactide–polycaprolactone copolymer composite: Mechanical properties and cytocompatibility with osteoblasts and macrophages in vitro
journal, September 2010

Assembly of Graphene Sheets into 3D Macroscopic Structures
journal, May 2012

Cell specific cytotoxicity and uptake of graphene nanoribbons
journal, January 2013

Plasma-treated polystyrene surfaces: model surfaces for studying cell–biomaterial interactions
journal, May 2004

Raman Spectroscopy Of Boron Nitride Nanotubes And Boron Nitride — Carbon Composites
conference, January 2005

Interfacial modification of boron nitride nanoplatelets for epoxy composites with improved thermal properties
journal, January 2012

Apatite formability of boron nitride nanotubes
journal, March 2011

Injectable Nanocomposites of Single-Walled Carbon Nanotubes and Biodegradable Polymers for Bone Tissue Engineering
journal, July 2006

Controlled surface modification of boron nitride nanotubes
journal, April 2011

Fabrication and in vitro degradation of porous fumarate-based polymer/alumoxane nanocomposite scaffolds for bone tissue engineering
journal, April 2009

Effective mechanical properties of hexagonal boron nitride nanosheets
journal, November 2011

Degradation and biocompatibility of a poly(propylene fumarate)-based/alumoxane nanocomposite for bone tissue engineering
journal, January 2007

Porous boron nitride nanosheets for effective water cleaning
journal, April 2013

In vitro degradation of a poly(propylene fumarate)-based composite material
journal, November 1996

Synthesis, characterization, in vitro phantom imaging, and cytotoxicity of a novel graphene-based multimodal magnetic resonance imaging-X-ray computed tomography contrast agent
journal, January 2014

Interactions of 1D- and 2D-layered inorganic nanoparticles with fibroblasts and human mesenchymal stem cells
journal, June 2015

Soluble, Exfoliated Hexagonal Boron Nitride Nanosheets
journal, November 2009

In Vitro Cytotoxicity of Injectable and Biodegradable Poly(propylene fumarate)-Based Networks: Unreacted Macromers, Cross-Linked Networks, and Degradation Products
journal, July 2003

Recent progress in the research of inorganic fullerene-like nanoparticles and inorganic nanotubes
journal, January 2010

Young modulus, mechanical and electrical properties of isolated individual and bundled single-walled boron nitride nanotubes
journal, May 2011

Isolation and Characterization of MC3T3-E1 Preosteoblast Subclones with Distinct In Vitro and In Vivo Differentiation/Mineralization Potential
journal, June 1999

Polymer composites of boron nitride nanotubes and nanosheets
journal, January 2014

Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells
journal, January 2014

Catalyzed Collapse and Enhanced Hydrogen Storage of BN Nanotubes
journal, December 2002

Injectable in situ cross-linkable nanocomposites of biodegradable polymers and carbon nanostructures for bone tissue engineering
journal, January 2007

In vitro cytotoxicity of single‐walled carbon nanotube/biodegradable polymer nanocomposites
journal, September 2008

Thermal Diffusivity of Hexagonal Boron Nitride Composites Based on Cross-Linked Liquid Crystalline Polyimides
journal, January 2013

Fibrin(ogen) mediates acute inflammatory responses to biomaterials
journal, December 1993

Boron nitride nanotubes: functionalization and composites
journal, January 2008

Insight into reactions and interface between boron nitride nanotube and aluminum
journal, September 2012

Graphene-based contrast agents for photoacoustic and thermoacoustic tomography
journal, December 2013

Mechanical and electronic properties of carbon and boron–nitride nanotubes
journal, January 2000

Fabrication and characterization of three-dimensional macroscopic all-carbon scaffolds
journal, March 2013

In Vitro Investigation of the Cellular Toxicity of Boron Nitride Nanotubes
journal, April 2011

The effects of graphene nanostructures on mesenchymal stem cells
journal, June 2014

Promises, facts and challenges for carbon nanotubes in imaging and therapeutics
journal, September 2009

Synthesis of poly(propylene fumarate)
journal, April 2009

A review of particulate reinforcement theories for polymer composites
journal, December 1990

In Vitro Degradation of a Poly(Propylene Fumarate)/ β -Tricalcium Phosphate Composite Orthopaedic Scaffold
journal, June 1997

Role of the benzoyloxyl radical in DNA damage mediated by benzoyl peroxide
journal, March 1991

In vitro cytocompatibility of one-dimensional and two-dimensional nanostructure-reinforced biodegradable polymeric nanocomposites
journal, November 2014

Boron nitride nanotube reinforced hydroxyapatite composite: Mechanical and tribological performance and in-vitro biocompatibility to osteoblasts
journal, January 2011

Two-Dimensional Nanostructure-Reinforced Biodegradable Polymeric Nanocomposites for Bone Tissue Engineering
journal, February 2013

Nanoreinforcement of Poly(propylene fumarate)-Based Networks with Surface Modified Alumoxane Nanoparticles for Bone Tissue Engineering
journal, September 2004

Fibronectin-integrin interactions
journal, January 1997

The Bicinchoninic Acid (BCA) Assay for Protein Quantitation
book, January 2009

Two-dimensional graphene analogues for biomedical applications
journal, January 2015

Enhancement of Protein Adsorption Induced by Surface Roughness
journal, December 2006

Preparation of poly(vinyl alcohol) nanocomposites with molybdenum disulfide (MoS2): structural characteristics and markedly enhanced properties
journal, January 2012

The reliability and limits of the MTT reduction assay for carbon nanotubes–cell interaction
journal, November 2007

Adsorption of BSA on Highly Carboxylated Microspheres—Quantitative Effects of Surface Functional Groups and Interaction Forces
journal, February 1996

Measurement of protein adsorption kinetics by an in situ, “real-time,” solution depletion technique
journal, April 1992

Large Scale Growth and Characterization of Atomic Hexagonal Boron Nitride Layers
journal, August 2010

Comparative theoretical study of single-wall carbon and boron-nitride nanotubes
journal, June 2003

Tungsten disulfide nanotubes reinforced biodegradable polymers for bone tissue engineering
journal, September 2013

Properties of Boron Nitride Nanotubes
conference, January 2003

A quick and simple method for the quantitation of lactate dehydrogenase release in measurements of cellular cytotoxicity and tumor necrosis factor (TNF) activity
journal, November 1988

Nanotube composites
journal, June 2007

Bone-Tissue-Engineering Material Poly(propylene fumarate): Correlation between Molecular Weight, Chain Dimensions, and Physical Properties
journal, June 2006

The effect of particle shape on the mechanical properties of filled polymers
journal, August 1980

Fabrication of Alumina Ceramic Reinforced with Boron Nitride Nanotubes with Improved Mechanical Properties
journal, September 2011