Evaluation of revised polymerase chain reaction primers for more inclusive quantification of ammonia‐oxidizing archaea and bacteria
Abstract
Summary Ammonia‐oxidizing archaea ( AOA ) and bacteria ( AOB ) fill key roles in the nitrogen cycle. Thus, well‐vetted methods for characterizing their distribution are essential for framing studies of their significance in natural and managed systems. Quantification of the gene coding for one subunit of the ammonia monooxygenase ( amoA ) by polymerase chain reaction is frequently employed to enumerate the two groups. However, variable amplification of sequence variants comprising this conserved genetic marker for ammonia oxidizers potentially compromises within‐ and between‐system comparisons. We compared the performance of newly designed non‐degenerate quantitative polymerase chain reaction primer sets to existing primer sets commonly used to quantify the amoA of AOA and AOB using a collection of plasmids and soil DNA samples. The new AOA primer set provided improved quantification of model mixtures of different amoA sequence variants and increased detection of amoA in DNA recovered from soils. Although both primer sets for the AOB provided similar results for many comparisons, the new primers demonstrated increased detection in environmental application. Thus, the new primer sets should provide a useful complement to primers now commonly used to characterize the environmental distribution of AOA and AOB .
- Authors:
-
- Civil and Environmental Engineering University of Washington Seattle WA 98105 USA
- School of Environmental and Forest Sciences University of Washington Seattle WA 98105 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1400677
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Environmental Microbiology Reports
- Additional Journal Information:
- Journal Name: Environmental Microbiology Reports Journal Volume: 7 Journal Issue: 2; Journal ID: ISSN 1758-2229
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Meinhardt, Kelley A., Bertagnolli, Anthony, Pannu, Manmeet W., Strand, Stuart E., Brown, Sally L., and Stahl, David A. Evaluation of revised polymerase chain reaction primers for more inclusive quantification of ammonia‐oxidizing archaea and bacteria. United States: N. p., 2015.
Web. doi:10.1111/1758-2229.12259.
Meinhardt, Kelley A., Bertagnolli, Anthony, Pannu, Manmeet W., Strand, Stuart E., Brown, Sally L., & Stahl, David A. Evaluation of revised polymerase chain reaction primers for more inclusive quantification of ammonia‐oxidizing archaea and bacteria. United States. https://doi.org/10.1111/1758-2229.12259
Meinhardt, Kelley A., Bertagnolli, Anthony, Pannu, Manmeet W., Strand, Stuart E., Brown, Sally L., and Stahl, David A. Thu .
"Evaluation of revised polymerase chain reaction primers for more inclusive quantification of ammonia‐oxidizing archaea and bacteria". United States. https://doi.org/10.1111/1758-2229.12259.
@article{osti_1400677,
title = {Evaluation of revised polymerase chain reaction primers for more inclusive quantification of ammonia‐oxidizing archaea and bacteria},
author = {Meinhardt, Kelley A. and Bertagnolli, Anthony and Pannu, Manmeet W. and Strand, Stuart E. and Brown, Sally L. and Stahl, David A.},
abstractNote = {Summary Ammonia‐oxidizing archaea ( AOA ) and bacteria ( AOB ) fill key roles in the nitrogen cycle. Thus, well‐vetted methods for characterizing their distribution are essential for framing studies of their significance in natural and managed systems. Quantification of the gene coding for one subunit of the ammonia monooxygenase ( amoA ) by polymerase chain reaction is frequently employed to enumerate the two groups. However, variable amplification of sequence variants comprising this conserved genetic marker for ammonia oxidizers potentially compromises within‐ and between‐system comparisons. We compared the performance of newly designed non‐degenerate quantitative polymerase chain reaction primer sets to existing primer sets commonly used to quantify the amoA of AOA and AOB using a collection of plasmids and soil DNA samples. The new AOA primer set provided improved quantification of model mixtures of different amoA sequence variants and increased detection of amoA in DNA recovered from soils. Although both primer sets for the AOB provided similar results for many comparisons, the new primers demonstrated increased detection in environmental application. Thus, the new primer sets should provide a useful complement to primers now commonly used to characterize the environmental distribution of AOA and AOB .},
doi = {10.1111/1758-2229.12259},
journal = {Environmental Microbiology Reports},
number = 2,
volume = 7,
place = {United States},
year = {Thu Feb 12 00:00:00 EST 2015},
month = {Thu Feb 12 00:00:00 EST 2015}
}
https://doi.org/10.1111/1758-2229.12259
Web of Science
Works referenced in this record:
Calculator Tool for Determining Greenhouse Gas Emissions for Biosolids Processing and End Use
journal, December 2010
- Brown, Sally; Beecher, Ned; Carpenter, Andrew
- Environmental Science & Technology, Vol. 44, Issue 24
Quantitative analyses of the abundance and composition of ammonia-oxidizing bacteria and ammonia-oxidizing archaea of a Chinese upland red soil under long-term fertilization practices
journal, September 2007
- He, Ji-zheng; Shen, Ju-pei; Zhang, Li-mei
- Environmental Microbiology, Vol. 9, Issue 9
Ammonium supply rate influences archaeal and bacterial ammonia oxidizers in a wetland soil vertical profile: Selection of ammonia oxidizers in wetland soil
journal, August 2010
- Höfferle, Špela; Nicol, Graeme W.; Pal, Levin
- FEMS Microbiology Ecology, Vol. 74, Issue 2
Nitrification driven by bacteria and not archaea in nitrogen-rich grassland soils
journal, August 2009
- Di, H. J.; Cameron, K. C.; Shen, J. P.
- Nature Geoscience, Vol. 2, Issue 9
Bias in Template-to-Product Ratios in Multitemplate PCR
journal, October 1998
- Polz, Martin F.; Cavanaugh, Colleen M.
- Applied and Environmental Microbiology, Vol. 64, Issue 10
Cultivation of a thermophilic ammonia oxidizing archaeon synthesizing crenarchaeol
journal, March 2008
- de la Torre, José R.; Walker, Christopher B.; Ingalls, Anitra E.
- Environmental Microbiology, Vol. 10, Issue 3
Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean
journal, September 2005
- Francis, C. A.; Roberts, K. J.; Beman, J. M.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 41, p. 14683-14688
High abundance of Crenarchaeota in a temperate acidic forest soil: Highly abundant Crenarchaeota in a forest soil
journal, June 2007
- Kemnitz, Dana; Kolb, Steffen; Conrad, Ralf
- FEMS Microbiology Ecology, Vol. 60, Issue 3
Effect of nitrification inhibitors on the growth and activity of Nitrosotalea devanaterra in culture and soil
journal, July 2013
- Lehtovirta-Morley, Laura E.; Verhamme, Daniël T.; Nicol, Graeme W.
- Soil Biology and Biochemistry, Vol. 62
Solutions for a cultivated planet
journal, October 2011
- Foley, Jonathan A.; Ramankutty, Navin; Brauman, Kate A.
- Nature, Vol. 478, Issue 7369
Community composition of ammonia-oxidizing bacteria and archaea in soils under stands of red alder and Douglas fir in Oregon
journal, November 2008
- Boyle-Yarwood, Stephanie A.; Bottomley, Peter J.; Myrold, David D.
- Environmental Microbiology, Vol. 10, Issue 11
Marine ammonia-oxidizing archaeal isolates display obligate mixotrophy and wide ecotypic variation
journal, August 2014
- Qin, W.; Amin, S. A.; Martens-Habbena, W.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 34
A moderately thermophilic ammonia-oxidizing crenarchaeote from a hot spring
journal, February 2008
- Hatzenpichler, R.; Lebedeva, E. V.; Spieck, E.
- Proceedings of the National Academy of Sciences, Vol. 105, Issue 6
Distinct Responses in Ammonia-Oxidizing Archaea and Bacteria after Addition of Biosolids to an Agricultural Soil
journal, July 2011
- Kelly, John J.; Policht, Katherine; Grancharova, Tanya
- Applied and Environmental Microbiology, Vol. 77, Issue 18
Archaea Dominate the Ammonia-Oxidizing Community in the Rhizosphere of the Freshwater Macrophyte Littorella uniflora
journal, March 2008
- Herrmann, M.; Saunders, A. M.; Schramm, A.
- Applied and Environmental Microbiology, Vol. 74, Issue 10
The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations.
journal, January 1997
- Rotthauwe, J. H.; Witzel, K. P.; Liesack, W.
- Applied and environmental microbiology, Vol. 63, Issue 12
Altitude ammonia-oxidizing bacteria and archaea in soils of Mount Everest
journal, November 2009
- Zhang, Li-Mei; Wang, Mu; Prosser, James I.
- FEMS Microbiology Ecology, Vol. 70, Issue 2
Ammonia oxidation kinetics determine niche separation of nitrifying Archaea and Bacteria
journal, September 2009
- Martens-Habbena, Willm; Berube, Paul M.; Urakawa, Hidetoshi
- Nature, Vol. 461, Issue 7266
Nitrososphaera viennensis, an ammonia oxidizing archaeon from soil
journal, April 2011
- Tourna, M.; Stieglmeier, M.; Spang, A.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 20
Bacteria rather than Archaea dominate microbial ammonia oxidation in an agricultural soil
journal, July 2009
- Jia, Zhongjun; Conrad, Ralf
- Environmental Microbiology, Vol. 11, Issue 7
The Nature and Longevity of Agricultural Impacts on Soil Carbon and Nutrients: A Review
journal, December 2006
- McLauchlan, Kendra
- Ecosystems, Vol. 9, Issue 8
Abundance of microbial genes associated with nitrogen cycling as indices of biogeochemical process rates across a vegetation gradient in Alaska: Functional genes predict potential N cycling rates
journal, January 2012
- Petersen, Dorthe Groth; Blazewicz, Steven J.; Firestone, Mary
- Environmental Microbiology, Vol. 14, Issue 4
Review of greenhouse gas emissions from crop production systems and fertilizer management effects
journal, October 2009
- Snyder, C. S.; Bruulsema, T. W.; Jensen, T. L.
- Agriculture, Ecosystems & Environment, Vol. 133, Issue 3-4
Archaea rather than bacteria control nitrification in two agricultural acidic soils: Archaeal nitrification in acidic soils
journal, October 2010
- Gubry-Rangin, Cécile; Nicol, Graeme W.; Prosser, James I.
- FEMS Microbiology Ecology, Vol. 74, Issue 3
Archaea predominate among ammonia-oxidizing prokaryotes in soils
journal, August 2006
- Leininger, S.; Urich, T.; Schloter, M.
- Nature, Vol. 442, Issue 7104
Nitrification rates in Arctic soils are associated with functionally distinct populations of ammonia-oxidizing archaea
journal, March 2013
- Alves, Ricardo J. Eloy; Wanek, Wolfgang; Zappe, Anna
- The ISME Journal, Vol. 7, Issue 8
The influence of soil pH on the diversity, abundance and transcriptional activity of ammonia oxidizing archaea and bacteria
journal, November 2008
- Nicol, Graeme W.; Leininger, Sven; Schleper, Christa
- Environmental Microbiology, Vol. 10, Issue 11
Evaluation of quantitative polymerase chain reaction-based approaches for determining gene copy and gene transcript numbers in environmental samples
journal, May 2006
- Smith, Cindy J.; Nedwell, David B.; Dong, Liang F.
- Environmental Microbiology, Vol. 8, Issue 5
Isolation of an autotrophic ammonia-oxidizing marine archaeon
journal, September 2005
- Könneke, Martin; Bernhard, Anne E.; de la Torre, José R.
- Nature, Vol. 437, Issue 7058
Cultivation of an obligate acidophilic ammonia oxidizer from a nitrifying acid soil
journal, September 2011
- Lehtovirta-Morley, L. E.; Stoecker, K.; Vilcinskas, A.
- Proceedings of the National Academy of Sciences, Vol. 108, Issue 38
Growth of ammonia-oxidizing archaea in soil microcosms is inhibited by acetylene: Archaeal ammonia oxidation in soil
journal, October 2009
- Offre, Pierre; Prosser, James I.; Nicol, Graeme W.
- FEMS Microbiology Ecology, Vol. 70, Issue 1
Novel genes for nitrite reductase and Amo-related proteins indicate a role of uncultivated mesophilic crenarchaeota in nitrogen cycling: Role of mesophilic crenarchaeota in nitrogen cycling
journal, September 2005
- Treusch, Alexander H.; Leininger, Sven; Kletzin, Arnulf
- Environmental Microbiology, Vol. 7, Issue 12
Quantitative distribution of presumptive archaeal and bacterial nitrifiers in Monterey Bay and the North Pacific Subtropical Gyre
journal, May 2007
- Mincer, Tracy J.; Church, Matthew J.; Taylor, Lance Trent
- Environmental Microbiology, Vol. 9, Issue 5
The degenerate primer design problem
journal, July 2002
- Linhart, C.; Shamir, R.
- Bioinformatics, Vol. 18, Issue Suppl 1
amoA-based consensus phylogeny of ammonia-oxidizing archaea and deep sequencing of amoA genes from soils of four different geographic regions: Archaeal amoA phylogeny and diversity in soils
journal, December 2011
- Pester, Michael; Rattei, Thomas; Flechl, Stefan
- Environmental Microbiology, Vol. 14, Issue 2