Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins

Jang, Yeongseon; Choi, Won Tae; Heller, William T.; Ke, Zunlong; Wright, Elizabeth R.; Champion, Julie A.

doi:10.1002/smll.201700399

Title: Engineering Globular Protein Vesicles through Tunable Self-Assembly of Recombinant Fusion Proteins

Journal Article · Thu Jul 27 04:00:00 UTC 2017 · Small

DOI: https://doi.org/10.1002/smll.201700399 · OSTI ID:1399972

Jang, Yeongseon ^[1]; Choi, Won Tae ^[1];

^[2]; Ke, Zunlong ^[3]; Wright, Elizabeth R. ^[4]; Champion, Julie A. ^[1]

Georgia Inst. of Technology, Atlanta, GA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Emory Univ. School of Medicine, Children's Healthcare of Atlanta, Atlanta, GA (United States); Georgia Inst. of Technology, Atlanta, GA (United States)
Emory Univ. School of Medicine, Children's Healthcare of Atlanta, Atlanta, GA (United States)

Vesicles assembled from folded, globular proteins have potential for functions different from traditional lipid or polymeric vesicles. However, they also present challenges in understanding the assembly process and controlling vesicle properties. From detailed investigation of the assembly behavior of recombinant fusion proteins, this work reports a simple strategy to engineer protein vesicles containing functional, globular domains. This is achieved through tunable self-assembly of recombinant globular fusion proteins containing leucine zippers and elastin-like polypeptides. The fusion proteins form complexes in solution via high affinity binding of the zippers, and transition through dynamic coacervates to stable hollow vesicles upon warming. The thermal driving force, which can be tuned by protein concentration or temperature, controls both vesicle size and whether vesicles are single or bi-layered. Lastly, these results provide critical information to engineer globular protein vesicles via self-assembly with desired size and membrane structure.

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Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1399972

Journal Information:: Small, Journal Name: Small Journal Issue: 36 Vol. 13; ISSN 1613-6810

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

References (33)

Topological Effects on Globular Protein-ELP Fusion Block Copolymer Self-Assembly Qin, Guokui; Glassman, Matthew J.; Lam, Christopher N. Advanced Functional Materials, Vol. 25, Issue 5 https://doi.org/10.1002/adfm.201403453	journal	December 2014
Two-Step Protein Self-Assembly in the Extracellular Matrix Park, Won Min; Champion, Julie A. Angewandte Chemie, Vol. 125, Issue 31 https://doi.org/10.1002/ange.201302331	journal	June 2013
Two-Step Protein Self-Assembly in the Extracellular Matrix Park, Won Min; Champion, Julie A. Angewandte Chemie International Edition, Vol. 52, Issue 31 https://doi.org/10.1002/anie.201302331	journal	June 2013
Recombinant Protein Technology Fothergill-Gilmore, Linda A. Protein Biotechnology https://doi.org/10.1007/978-1-59259-438-2_13	book	January 1993
The science and applications of emulsions — an overview Israelachvili, Jacob Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 91 https://doi.org/10.1016/0927-7757(94)02743-9	journal	November 1994
Liposomal drug delivery systems: From concept to clinical applications Allen, Theresa M.; Cullis, Pieter R. Advanced Drug Delivery Reviews, Vol. 65, Issue 1 https://doi.org/10.1016/j.addr.2012.09.037	journal	January 2013
Polymersomes for drug delivery: Design, formation and characterization Lee, Jung Seok; Feijen, Jan Journal of Controlled Release, Vol. 161, Issue 2 https://doi.org/10.1016/j.jconrel.2011.10.005	journal	July 2012
Self-Assembled Materials Made from Functional Recombinant Proteins Jang, Yeongseon; Champion, Julie A. Accounts of Chemical Research, Vol. 49, Issue 10 https://doi.org/10.1021/acs.accounts.6b00337	journal	September 2016
Techniques To Control Polymersome Size Bleul, Regina; Thiermann, Raphael; Maskos, Michael Macromolecules, Vol. 48, Issue 20 https://doi.org/10.1021/acs.macromol.5b01500	journal	October 2015
Polymeric Vesicles: From Drug Carriers to Nanoreactors and Artificial Organelles Tanner, Pascal; Baumann, Patric; Enea, Ramona Accounts of Chemical Research, Vol. 44, Issue 10 https://doi.org/10.1021/ar200036k	journal	October 2011
Quantification of the Effects of Chain Length and Concentration on the Thermal Behavior of Elastin-like Polypeptides Meyer, Dan E.; Chilkoti, Ashutosh Biomacromolecules, Vol. 5, Issue 3 https://doi.org/10.1021/bm034215n	journal	May 2004
Improved Non-chromatographic Purification of a Recombinant Protein by Cationic Elastin-like Polypeptides Lim, Dong Woo; Trabbic-Carlson, Kimberly; MacKay, J. Andrew Biomacromolecules, Vol. 8, Issue 5 https://doi.org/10.1021/bm060849t	journal	May 2007
Thermally Triggered Self-Assembly of Folded Proteins into Vesicles Park, Won Min; Champion, Julie A. Journal of the American Chemical Society, Vol. 136, Issue 52 https://doi.org/10.1021/ja5090157	journal	December 2014
Small-Angle Neutron-Scattering Studies of Nonionic Surfactant Vesicles Ma, G.; Barlow, D. J.; Lawrence, M. J. The Journal of Physical Chemistry B, Vol. 104, Issue 39, 9081-9085 https://doi.org/10.1021/jp000934	journal	August 2000
Small-Angle Neutron-Scattering Studies of Nonionic Surfactant Vesicles Ma, G.; Barlow, D. J.; Lawrence, M. J. The Journal of Physical Chemistry B, Vol. 104, Issue 39 https://doi.org/10.1021/jp000934+	journal	August 2000
Small-Angle Neutron Scattering (SANS) Study of Vesicles and Lamellar Sheets Formed from Mixtures of an Anionic and a Cationic Surfactant Bergström, Magnus; Pedersen, Jan Skov; Schurtenberger, Peter The Journal of Physical Chemistry B, Vol. 103, Issue 45 https://doi.org/10.1021/jp991846w	journal	November 1999
Are Thermoresponsive Microgels Model Systems for Concentrated Colloidal Suspensions? A Rheology and Small-Angle Neutron Scattering Study Stieger, Markus; Pedersen, Jan Skov; Lindner, Peter Langmuir, Vol. 20, Issue 17 https://doi.org/10.1021/la049518x	journal	August 2004
Templated formation of giant polymer vesicles with controlled size distributions Howse, Jonathan R.; Jones, Richard A. L.; Battaglia, Giuseppe Nature Materials, Vol. 8, Issue 6 https://doi.org/10.1038/nmat2446	journal	May 2009
Thermo-responsive and fluorescent cellulose nanocrystals grafted with polymer brushes Wu, Weibing; Huang, Fang; Pan, Shaobo Journal of Materials Chemistry A, Vol. 3, Issue 5 https://doi.org/10.1039/C4TA04761C	journal	January 2015
Self-assembling dual component nanoparticles with endosomal escape capability Wong, Adelene S. M.; Mann, Sarah K.; Czuba, Ewa Soft Matter, Vol. 11, Issue 15 https://doi.org/10.1039/C5SM00082C	journal	January 2015
Polymeric vesicles in biomedical applications Brinkhuis, René P.; Rutjes, Floris P. J. T.; van Hest, Jan C. M. Polymer Chemistry, Vol. 2, Issue 7 https://doi.org/10.1039/c1py00061f	journal	January 2011
One-step preparation of giant lipid vesicles with high encapsulation efficiency using an electrospray technique Maruyama, Tatsuo; Fukui, Yu; Tsuchiya, Eiko RSC Advances, Vol. 2, Issue 31 https://doi.org/10.1039/c2ra21423g	journal	January 2012
Self-assembly of tunable protein suprastructures from recombinant oleosin Vargo, K. B.; Parthasarathy, R.; Hammer, D. A. Proceedings of the National Academy of Sciences, Vol. 109, Issue 29 https://doi.org/10.1073/pnas.1205426109	journal	July 2012
Effect of protein fusion on the transition temperature of an environmentally responsive elastin-like polypeptide: a role for surface hydrophobicity? Trabbic-Carlson, K.; Meyer, D. E.; Liu, L. Protein Engineering Design and Selection, Vol. 17, Issue 1 https://doi.org/10.1093/protein/gzh006	journal	January 2004
An introduction to small-angle neutron scattering Windsor, C. G. Journal of Applied Crystallography, Vol. 21, Issue 6 https://doi.org/10.1107/S0021889888008404	journal	December 1988
Designed heterodimerizing leucine zippers with a ranger of pIs and stabilities up to 10-15 M Moll, J. R. Protein Science, Vol. 10, Issue 3 https://doi.org/10.1110/ps.39401	journal	March 2001
Self-Assembly of Mesoscopic Metal-Polymer Amphiphiles Park, S. Science, Vol. 303, Issue 5656 https://doi.org/10.1126/science.1093276	journal	January 2004
Self-Assembled Aggregates of Rod-Coil Block Copolymers and Their Solubilization and Encapsulation of Fullerenes Jenekhe, S. A. Science, Vol. 279, Issue 5358 https://doi.org/10.1126/science.279.5358.1903	journal	March 1998
Polymersomes: Tough Vesicles Made from Diblock Copolymers Discher, B. M. Science, Vol. 284, Issue 5417 https://doi.org/10.1126/science.284.5417.1143	journal	May 1999
Amphiphiles Self-Assembly: Basic Concepts and Future Perspectives of Supramolecular Approaches Lombardo, Domenico; Kiselev, Mikhail A.; Magazù, Salvatore Advances in Condensed Matter Physics, Vol. 2015 https://doi.org/10.1155/2015/151683	journal	January 2015
Liposome: classification, preparation, and applications Akbarzadeh, Abolfazl; Rezaei-Sadabady, Rogaie; Davaran, Soodabeh Nanoscale Research Letters, Vol. 8, Issue 1 https://doi.org/10.1186/1556-276X-8-102	journal	February 2013
Limiting factors in single particle cryo electron tomography Kudryashev, Mikhail; Castaño-Díez, Daniel; Stahlberg, Henning Research Network of Computational and Structural Biotechnology https://doi.org/10.5167/uzh-97290	text	January 2012
Limiting Factors in Single Particle cryo Electron Tomography Kudryashev, Mikhail; Castaño-Díez, Daniel; Stahlberg, Henning Computational and Structural Biotechnology Journal, Vol. 1, Issue 2 https://doi.org/10.5936/csbj.201207002	journal	July 2012

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Complex microparticle architectures from stimuli-responsive intrinsically disordered proteins Roberts, Stefan; Miao, Vincent; Costa, Simone Nature Communications, Vol. 11, Issue 1 https://doi.org/10.1038/s41467-020-15128-9	journal	March 2020
Engineering the Architecture of Elastin‐Like Polypeptides: From Unimers to Hierarchical Self‐Assembly Saha, Soumen; Banskota, Samagya; Roberts, Stefan Advanced Therapeutics, Vol. 3, Issue 3 https://doi.org/10.1002/adtp.201900164	journal	February 2020
Effect of peptide linker length and composition on immobilization and catalysis of leucine zipper-enzyme fusion proteins Caparco, Adam A.; Bommarius, Andreas S.; Champion, Julie A. AIChE Journal, Vol. 64, Issue 8 https://doi.org/10.1002/aic.16150	journal	March 2018
Self-assembly of amphiphilic truncated cones to form hollow nanovesicles Wang, Yali; He, Xuehao RSC Advances, Vol. 8, Issue 24 https://doi.org/10.1039/c8ra01100a	journal	January 2018

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