Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo
Abstract
The realization that nuclear distribution of DNA, RNA, and proteins differs between cell types and developmental stages suggests that nuclear organization serves regulatory functions. Understanding the logic of nuclear architecture and how it contributes to differentiation and cell fate commitment remains challenging. Here, we use soft X-ray tomography (SXT) to image chromatin organization, distribution, and biophysical properties during neurogenesis in vivo. Our analyses reveal that chromatin with similar biophysical properties forms an elaborate connected network throughout the entire nucleus. Although this interconnectivity is present in every developmental stage, differentiation proceeds with concomitant increase in chromatin compaction and re-distribution of condensed chromatin toward the nuclear core. HP1β, but not nucleosome spacing or phasing, regulates chromatin rearrangements because it governs both the compaction of chromatin and its interactions with the nuclear envelope. Our experiments introduce SXT as a powerful imaging technology for nuclear architecture.
- Authors:
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1399023
- Alternate Identifier(s):
- OSTI ID: 1377588
- Grant/Contract Number:
- AC02-5CH11231; AC02-05CH11231; R01DA030320; U01DA040582
- Resource Type:
- Published Article
- Journal Name:
- Cell Reports
- Additional Journal Information:
- Journal Name: Cell Reports Journal Volume: 17 Journal Issue: 8; Journal ID: ISSN 2211-1247
- Publisher:
- Elsevier
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 46 INSTRUMENTATION RELATED TO NUCLEAR SCIENCE AND TECHNOLOGY; chromatin; nucleus; soft X-ray tomography; olfactory sensory neurons; nuclear organization; differentiation; neurogenesis
Citation Formats
Le Gros, Mark A., Clowney, E. Josephine, Magklara, Angeliki, Yen, Angela, Markenscoff-Papadimitriou, Eirene, Colquitt, Bradley, Myllys, Markko, Kellis, Manolis, Lomvardas, Stavros, and Larabell, Carolyn A. Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo. Netherlands: N. p., 2016.
Web. doi:10.1016/j.celrep.2016.10.060.
Le Gros, Mark A., Clowney, E. Josephine, Magklara, Angeliki, Yen, Angela, Markenscoff-Papadimitriou, Eirene, Colquitt, Bradley, Myllys, Markko, Kellis, Manolis, Lomvardas, Stavros, & Larabell, Carolyn A. Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo. Netherlands. https://doi.org/10.1016/j.celrep.2016.10.060
Le Gros, Mark A., Clowney, E. Josephine, Magklara, Angeliki, Yen, Angela, Markenscoff-Papadimitriou, Eirene, Colquitt, Bradley, Myllys, Markko, Kellis, Manolis, Lomvardas, Stavros, and Larabell, Carolyn A. Tue .
"Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo". Netherlands. https://doi.org/10.1016/j.celrep.2016.10.060.
@article{osti_1399023,
title = {Soft X-Ray Tomography Reveals Gradual Chromatin Compaction and Reorganization during Neurogenesis In Vivo},
author = {Le Gros, Mark A. and Clowney, E. Josephine and Magklara, Angeliki and Yen, Angela and Markenscoff-Papadimitriou, Eirene and Colquitt, Bradley and Myllys, Markko and Kellis, Manolis and Lomvardas, Stavros and Larabell, Carolyn A.},
abstractNote = {The realization that nuclear distribution of DNA, RNA, and proteins differs between cell types and developmental stages suggests that nuclear organization serves regulatory functions. Understanding the logic of nuclear architecture and how it contributes to differentiation and cell fate commitment remains challenging. Here, we use soft X-ray tomography (SXT) to image chromatin organization, distribution, and biophysical properties during neurogenesis in vivo. Our analyses reveal that chromatin with similar biophysical properties forms an elaborate connected network throughout the entire nucleus. Although this interconnectivity is present in every developmental stage, differentiation proceeds with concomitant increase in chromatin compaction and re-distribution of condensed chromatin toward the nuclear core. HP1β, but not nucleosome spacing or phasing, regulates chromatin rearrangements because it governs both the compaction of chromatin and its interactions with the nuclear envelope. Our experiments introduce SXT as a powerful imaging technology for nuclear architecture.},
doi = {10.1016/j.celrep.2016.10.060},
journal = {Cell Reports},
number = 8,
volume = 17,
place = {Netherlands},
year = {Tue Nov 01 00:00:00 EDT 2016},
month = {Tue Nov 01 00:00:00 EDT 2016}
}
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https://doi.org/10.1016/j.celrep.2016.10.060
https://doi.org/10.1016/j.celrep.2016.10.060
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Works referencing / citing this record:
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