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Title: Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction

A pilot-scale field experiment demonstrated that a one-time amendment of emulsified vegetable oil (EVO) reduced groundwater U(VI) concentrations for 1 year in a fast-flowing aquifer. However, little is known about how EVO amendment stimulates the functional gene composition, structure, and dynamics of groundwater microbial communities toward prolonged U(VI) reduction. In this paper, we hypothesized that EVO amendment would shift the functional gene composition and structure of groundwater microbial communities and stimulate key functional genes/groups involved in EVO biodegradation and reduction of electron acceptors in the aquifer. To test these hypotheses, groundwater microbial communities after EVO amendment were analyzed using a comprehensive functional gene microarray. Our results showed that EVO amendment stimulated sequential shifts in the functional composition and structure of groundwater microbial communities. Particularly, the relative abundance of key functional genes/groups involved in EVO biodegradation and the reduction of NO 3 -, Mn(IV), Fe(III), U(VI), and SO 4 2- significantly increased, especially during the active U(VI) reduction period. The relative abundance for some of these key functional genes/groups remained elevated over 9 months. Montel tests suggested that the dynamics in the abundance, composition, and structure of these key functional genes/groups were significantly correlated with groundwater concentrations of acetate, NO 3more » -, Mn(II), Fe(II), U(VI), and SO 4 2-. Our results suggest that EVO amendment stimulated dynamic succession of key functional microbial communities. Finally, this study improves our understanding of the composition, structure, and function changes needed for groundwater microbial communities to sustain a long-term U(VI) reduction.« less
Authors:
 [1] ;  [2] ;  [1] ;  [1] ;  [1] ;  [3] ;  [3] ;  [3] ;  [3] ;  [3] ;  [2] ;  [3] ;  [4] ;  [4] ;  [5] ;  [6]
  1. Univ. of Oklahoma, Norman, OK (United States)
  2. Stanford Univ., CA (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Michigan State Univ., East Lansing, MI (United States)
  5. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
  6. Univ. of Oklahoma, Norman, OK (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Tsinghua Univ., Beijing (China)
Publication Date:
Grant/Contract Number:
AC05-00OR22725; AC02-05CH11231; FG02-07ER64398
Type:
Accepted Manuscript
Journal Name:
Applied and Environmental Microbiology
Additional Journal Information:
Journal Volume: 81; Journal Issue: 12; Journal ID: ISSN 0099-2240
Publisher:
American Society for Microbiology
Research Org:
Univ. of Oklahoma, Norman, OK (United States); Michigan State Univ., East Lansing, MI (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
54 ENVIRONMENTAL SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
OSTI Identifier:
1394408

Zhang, Ping, Wu, Wei-Min, Van Nostrand, Joy D., Deng, Ye, He, Zhili, Gihring, Thomas, Zhang, Gengxin, Schadt, Chris W., Watson, David, Jardine, Phil, Criddle, Craig S., Brooks, Scott, Marsh, Terence L., Tiedje, James M., Arkin, Adam P., and Zhou, Jizhong. Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction. United States: N. p., Web. doi:10.1128/AEM.00043-15.
Zhang, Ping, Wu, Wei-Min, Van Nostrand, Joy D., Deng, Ye, He, Zhili, Gihring, Thomas, Zhang, Gengxin, Schadt, Chris W., Watson, David, Jardine, Phil, Criddle, Craig S., Brooks, Scott, Marsh, Terence L., Tiedje, James M., Arkin, Adam P., & Zhou, Jizhong. Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction. United States. doi:10.1128/AEM.00043-15.
Zhang, Ping, Wu, Wei-Min, Van Nostrand, Joy D., Deng, Ye, He, Zhili, Gihring, Thomas, Zhang, Gengxin, Schadt, Chris W., Watson, David, Jardine, Phil, Criddle, Craig S., Brooks, Scott, Marsh, Terence L., Tiedje, James M., Arkin, Adam P., and Zhou, Jizhong. 2015. "Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction". United States. doi:10.1128/AEM.00043-15. https://www.osti.gov/servlets/purl/1394408.
@article{osti_1394408,
title = {Dynamic Succession of Groundwater Functional Microbial Communities in Response to Emulsified Vegetable Oil Amendment during Sustained In Situ U(VI) Reduction},
author = {Zhang, Ping and Wu, Wei-Min and Van Nostrand, Joy D. and Deng, Ye and He, Zhili and Gihring, Thomas and Zhang, Gengxin and Schadt, Chris W. and Watson, David and Jardine, Phil and Criddle, Craig S. and Brooks, Scott and Marsh, Terence L. and Tiedje, James M. and Arkin, Adam P. and Zhou, Jizhong},
abstractNote = {A pilot-scale field experiment demonstrated that a one-time amendment of emulsified vegetable oil (EVO) reduced groundwater U(VI) concentrations for 1 year in a fast-flowing aquifer. However, little is known about how EVO amendment stimulates the functional gene composition, structure, and dynamics of groundwater microbial communities toward prolonged U(VI) reduction. In this paper, we hypothesized that EVO amendment would shift the functional gene composition and structure of groundwater microbial communities and stimulate key functional genes/groups involved in EVO biodegradation and reduction of electron acceptors in the aquifer. To test these hypotheses, groundwater microbial communities after EVO amendment were analyzed using a comprehensive functional gene microarray. Our results showed that EVO amendment stimulated sequential shifts in the functional composition and structure of groundwater microbial communities. Particularly, the relative abundance of key functional genes/groups involved in EVO biodegradation and the reduction of NO3-, Mn(IV), Fe(III), U(VI), and SO42- significantly increased, especially during the active U(VI) reduction period. The relative abundance for some of these key functional genes/groups remained elevated over 9 months. Montel tests suggested that the dynamics in the abundance, composition, and structure of these key functional genes/groups were significantly correlated with groundwater concentrations of acetate, NO3-, Mn(II), Fe(II), U(VI), and SO42-. Our results suggest that EVO amendment stimulated dynamic succession of key functional microbial communities. Finally, this study improves our understanding of the composition, structure, and function changes needed for groundwater microbial communities to sustain a long-term U(VI) reduction.},
doi = {10.1128/AEM.00043-15},
journal = {Applied and Environmental Microbiology},
number = 12,
volume = 81,
place = {United States},
year = {2015},
month = {4}
}