177 Lu-Labeled Phosphoramidate-Based PSMA Inhibitors: The Effect of an Albumin Binder on Biodistribution and Therapeutic Efficacy in Prostate Tumor-Bearing Mice
Abstract
Prostate-specific membrane antigen (PSMA) continues to be an active biomarker for small-molecule PSMA-targeted imaging and therapeutic agents for prostate cancer and various non-prostatic tumors that are characterized by PSMA expression on their neovasculature. One of the challenges for small-molecule PSMA inhibitors with respect to delivering therapeutic payloads is their rapid renal clearance. In order to overcome this pharmacokinetic challenge, we outfitted a 177Lu-labeled phosphoramidate-based PSMA inhibitor (CTT1298) with an albumin-binding motif (CTT1403) and compared its in vivo performance with that of an analogous compound lacking the albumin-binding motif (CTT1401). The radiolabeling of CTT1401 and CTT1403 was achieved using click chemistry to connect 177Lu-DOTA-N3 to the dibenzocyclooctyne (DBCO)-bearing CTT1298 inhibitor cores. A direct comparison in vitro and in vivo performance was made for CTT1401 and CTT1403; the specificity and efficacy by means of cellular uptake and internalization, biodistribution, and therapeutic efficacy were determined for both compounds. And while both compounds displayed excellent uptake and rapid internalization in PSMA+ PC3-PIP cells, the albumin binding moiety in CTT1403 conferred clear advantages to the PSMA-inhibitor scaffold including increased circulating half-life and prostate tumor uptake that continued to increase up to 168 h post-injection. This then increased tumor uptake translated into superior therapeutic efficacy ofmore »
- Authors:
-
- Cancer Targeted Technology, Woodinville, WA (United States)
- Univ. of Pittsburgh, PA (United States). Dept. of Medicine
- Univ. of Pittsburgh, PA (United States). Dept. of Medicine, Dept. of Radiology, Dept. of Bioengineering, Dept. of Pharmacology and Chemical Biology
- Publication Date:
- Research Org.:
- Univ. of Pittsburgh, PA (United States)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1393445
- Grant/Contract Number:
- SC0008833; HHSN261201500074C
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Theranostics
- Additional Journal Information:
- Journal Volume: 7; Journal Issue: 7; Journal ID: ISSN 1838-7640
- Publisher:
- Ivyspring
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; PSMA; albumin; phosphoramidate; 177Lu; radiotherapy
Citation Formats
Choy, Cindy J., Ling, Xiaoxi, Geruntho, Jonathan J., Beyer, Sophia K., Latoche, Joseph D., Langton-Webster, Beatrice, Anderson, Carolyn J., and Berkman, Clifford E. 177 Lu-Labeled Phosphoramidate-Based PSMA Inhibitors: The Effect of an Albumin Binder on Biodistribution and Therapeutic Efficacy in Prostate Tumor-Bearing Mice. United States: N. p., 2017.
Web. doi:10.7150/thno.18719.
Choy, Cindy J., Ling, Xiaoxi, Geruntho, Jonathan J., Beyer, Sophia K., Latoche, Joseph D., Langton-Webster, Beatrice, Anderson, Carolyn J., & Berkman, Clifford E. 177 Lu-Labeled Phosphoramidate-Based PSMA Inhibitors: The Effect of an Albumin Binder on Biodistribution and Therapeutic Efficacy in Prostate Tumor-Bearing Mice. United States. https://doi.org/10.7150/thno.18719
Choy, Cindy J., Ling, Xiaoxi, Geruntho, Jonathan J., Beyer, Sophia K., Latoche, Joseph D., Langton-Webster, Beatrice, Anderson, Carolyn J., and Berkman, Clifford E. Thu .
"177 Lu-Labeled Phosphoramidate-Based PSMA Inhibitors: The Effect of an Albumin Binder on Biodistribution and Therapeutic Efficacy in Prostate Tumor-Bearing Mice". United States. https://doi.org/10.7150/thno.18719. https://www.osti.gov/servlets/purl/1393445.
@article{osti_1393445,
title = {177 Lu-Labeled Phosphoramidate-Based PSMA Inhibitors: The Effect of an Albumin Binder on Biodistribution and Therapeutic Efficacy in Prostate Tumor-Bearing Mice},
author = {Choy, Cindy J. and Ling, Xiaoxi and Geruntho, Jonathan J. and Beyer, Sophia K. and Latoche, Joseph D. and Langton-Webster, Beatrice and Anderson, Carolyn J. and Berkman, Clifford E.},
abstractNote = {Prostate-specific membrane antigen (PSMA) continues to be an active biomarker for small-molecule PSMA-targeted imaging and therapeutic agents for prostate cancer and various non-prostatic tumors that are characterized by PSMA expression on their neovasculature. One of the challenges for small-molecule PSMA inhibitors with respect to delivering therapeutic payloads is their rapid renal clearance. In order to overcome this pharmacokinetic challenge, we outfitted a 177Lu-labeled phosphoramidate-based PSMA inhibitor (CTT1298) with an albumin-binding motif (CTT1403) and compared its in vivo performance with that of an analogous compound lacking the albumin-binding motif (CTT1401). The radiolabeling of CTT1401 and CTT1403 was achieved using click chemistry to connect 177Lu-DOTA-N3 to the dibenzocyclooctyne (DBCO)-bearing CTT1298 inhibitor cores. A direct comparison in vitro and in vivo performance was made for CTT1401 and CTT1403; the specificity and efficacy by means of cellular uptake and internalization, biodistribution, and therapeutic efficacy were determined for both compounds. And while both compounds displayed excellent uptake and rapid internalization in PSMA+ PC3-PIP cells, the albumin binding moiety in CTT1403 conferred clear advantages to the PSMA-inhibitor scaffold including increased circulating half-life and prostate tumor uptake that continued to increase up to 168 h post-injection. This then increased tumor uptake translated into superior therapeutic efficacy of CTT1403 in PSMA+ PC3-PIP human xenograft tumors.},
doi = {10.7150/thno.18719},
journal = {Theranostics},
number = 7,
volume = 7,
place = {United States},
year = {Thu Apr 27 00:00:00 EDT 2017},
month = {Thu Apr 27 00:00:00 EDT 2017}
}
Web of Science
Works referenced in this record:
A Portable Albumin Binder from a DNA-Encoded Chemical Library
journal, April 2008
- Dumelin, Christoph E.; Trüssel, Sabrina; Buller, Fabian
- Angewandte Chemie, Vol. 120, Issue 17
Induction of autoantibodies to syngeneic prostate-specific membrane antigen by xenogeneic vaccination
journal, January 2005
- Gregor, Polly D.; Wolchok, Jedd D.; Turaga, Vandana
- International Journal of Cancer, Vol. 116, Issue 3
Detection and characterization of the prostate-specific membrane antigen (PSMA) in tissue extracts and body fluids
journal, September 1995
- Trover, John K.; Beckett, Mary Lou; Wright, George L.
- International Journal of Cancer, Vol. 62, Issue 5
Correspondence Re: Ghosh A and Heston WDW. Tumor target prostate specific membrane antigen (PSMA) and its regulation in prostate cancer. J Cell Biochem 91:528-539, 2004
journal, October 2004
- Cardillo, M. R.; Gentile, V.; Di Silverio, F.
- Journal of Cellular Biochemistry, Vol. 93, Issue 4
Anti-tumor effects of toxins targeted to the prostate specific membrane antigen
journal, August 2002
- Fracasso, Giulio; Bellisola, Giuseppe; Cingarlini, Sara
- The Prostate, Vol. 53, Issue 1
Comparative study of PSMA expression in the prostate of mouse, dog, monkey, and human
journal, June 2006
- Aggarwal, Saurabh; Ricklis, Rebecca M.; Williams, Simon A.
- The Prostate, Vol. 66, Issue 9
Cell-Surface labeling and internalization by a fluorescent inhibitor of prostate-specific membrane antigen
journal, January 2008
- Liu, Tiancheng; Wu, Lisa Y.; Kazak, Marat
- The Prostate, Vol. 68, Issue 9
In vitro targeted photodynamic therapy with a pyropheophorbide-a conjugated inhibitor of prostate-specific membrane antigen
journal, May 2009
- Liu, Tiancheng; Wu, Lisa Y.; Choi, Joseph K.
- The Prostate, Vol. 69, Issue 6
PSMA-targeted SPECT agents: Mode of binding effect on in vitro performance
journal, August 2012
- Nedrow-Byers, Jessie R.; Moore, Adam L.; Ganguly, Tanushree
- The Prostate, Vol. 73, Issue 4
Optimising conditions for radiolabelling of DOTA-peptides with 90Y, 111In and 177Lu at high specific activities
journal, April 2003
- Breeman, Wouter A. P.; Jong, Marion; Visser, Theo J.
- European Journal of Nuclear Medicine and Molecular Imaging, Vol. 30, Issue 6
Cloning, expression, genomic localization, and enzymatic activities of the mouse homolog of prostate-specific membrane antigen/NAALADase/folate hydrolase
journal, February 2001
- Bacich, Dean J.; Pinto, John T.; Tong, William P.
- Mammalian Genome, Vol. 12, Issue 2
Targeting PSMA with a Cu-64 Labeled Phosphoramidate Inhibitor for PET/CT Imaging of Variant PSMA-Expressing Xenografts in Mouse Models of Prostate Cancer
journal, November 2015
- Nedrow, Jessie R.; Latoche, Joseph D.; Day, Kathryn E.
- Molecular Imaging and Biology, Vol. 18, Issue 3
The EPR effect for macromolecular drug delivery to solid tumors: Improvement of tumor uptake, lowering of systemic toxicity, and distinct tumor imaging in vivo
journal, January 2013
- Maeda, Hiroshi; Nakamura, Hideaki; Fang, Jun
- Advanced Drug Delivery Reviews, Vol. 65, Issue 1
Application of single-vial ready-for-use formulation of 111In- or 177Lu-labelled somatostatin analogs
journal, February 2014
- de Blois, Erik; Chan, Ho Sze; de Zanger, Rory
- Applied Radiation and Isotopes, Vol. 85
Labeling tumor cells with fluorescent nanocrystal–aptamer bioconjugates
journal, April 2006
- Chu, Ted C.; Shieh, Felice; Lavery, Laura A.
- Biosensors and Bioelectronics, Vol. 21, Issue 10
A targeted low molecular weight near-infrared fluorescent probe for prostate cancer
journal, December 2010
- Liu, Tiancheng; Wu, Lisa Y.; Hopkins, Mark R.
- Bioorganic & Medicinal Chemistry Letters, Vol. 20, Issue 23
Spacer length effects on in vitro imaging and surface accessibility of fluorescent inhibitors of prostate specific membrane antigen
journal, December 2011
- Liu, Tiancheng; Nedrow-Byers, Jessie R.; Hopkins, Mark R.
- Bioorganic & Medicinal Chemistry Letters, Vol. 21, Issue 23
Prostate-specific membrane antigen-targeted photodynamic therapy induces rapid cytoskeletal disruption
journal, October 2010
- Liu, Tiancheng; Wu, Lisa Y.; Berkman, Clifford E.
- Cancer Letters, Vol. 296, Issue 1
Progress in the discovery and development of glutamate carboxypeptidase II inhibitors
journal, September 2007
- Tsukamoto, Takashi; Wozniak, Krystyna M.; Slusher, Barbara S.
- Drug Discovery Today, Vol. 12, Issue 17-18
A clinical update of using albumin as a drug vehicle — A commentary
journal, September 2014
- Kratz, Felix
- Journal of Controlled Release, Vol. 190
Glutamate carboxypeptidase II levels in rodent brain using [125I]DCIT quantitative autoradiography
journal, October 2005
- Guilarte, Tomás R.; McGlothan, Jennifer L.; Foss, Catherine A.
- Neuroscience Letters, Vol. 387, Issue 3
A high-affinity [18F]-labeled phosphoramidate peptidomimetic PSMA-targeted inhibitor for PET imaging of prostate cancer
journal, October 2015
- Ganguly, Tanushree; Dannoon, Shorouk; Hopkins, Mark R.
- Nuclear Medicine and Biology, Vol. 42, Issue 10
Folate receptor-targeted radionuclide therapy: preclinical investigation of anti-tumor effects and potential radionephropathy
journal, October 2015
- Haller, Stephanie; Reber, Josefine; Brandt, Simone
- Nuclear Medicine and Biology, Vol. 42, Issue 10
Comparison of anti-prostate-specific membrane antigen antibodies and other immunomarkers in metastatic prostate carcinoma
journal, June 2001
- Chang, Sam S.; Reuter, Victor E.; Heston, W. D. W.
- Urology, Vol. 57, Issue 6
Microfluidic System for Studying the Interaction of Nanoparticles and Microparticles with Cells
journal, July 2005
- Farokhzad, Omid C.; Khademhosseini, Ali; Jon, Sangyong
- Analytical Chemistry, Vol. 77, Issue 17
High Resolution Time-of-Flight Mass Analysis of the Entire Range of Intact Singly-Charged Proteins
journal, November 2011
- Lee, Jeonghoon; Chen, Huijuan; Liu, Tiancheng
- Analytical Chemistry, Vol. 83, Issue 24
Structure–Activity Relationship of 18 F-Labeled Phosphoramidate Peptidomimetic Prostate-Specific Membrane Antigen (PSMA)-Targeted Inhibitor Analogues for PET Imaging of Prostate Cancer
journal, June 2016
- Dannoon, Shorouk; Ganguly, Tanushree; Cahaya, Hendry
- Journal of Medicinal Chemistry, Vol. 59, Issue 12
Preclinical Comparison of Albumin-Binding Radiofolates: Impact of Linker Entities on the in Vitro and in Vivo Properties
journal, January 2017
- Siwowska, Klaudia; Haller, Stephanie; Bortoli, Francesca
- Molecular Pharmaceutics, Vol. 14, Issue 2
Development of Inhibitor-Directed Enzyme Prodrug Therapy (IDEPT) for Prostate Cancer
journal, September 2014
- Martin, Stacy E.; Ganguly, Tanushree; Munske, Gerhard R.
- Bioconjugate Chemistry, Vol. 25, Issue 10
New Strategy for the Extension of the Serum Half-Life of Antibody Fragments
journal, November 2009
- Trüssel, Sabrina; Dumelin, Christoph; Frey, Katharina
- Bioconjugate Chemistry, Vol. 20, Issue 12
The resurgence of covalent drugs
journal, April 2011
- Singh, Juswinder; Petter, Russell C.; Baillie, Thomas A.
- Nature Reviews Drug Discovery, Vol. 10, Issue 4
Simple bioconjugate chemistry serves great clinical advances: albumin as a versatile platform for diagnosis and precision therapy
journal, January 2016
- Liu, Zhibo; Chen, Xiaoyuan
- Chemical Society Reviews, Vol. 45, Issue 5
Radioligand Therapy With 177Lu-PSMA-617 as A Novel Therapeutic Option in Patients With Metastatic Castration Resistant Prostate Cancer
journal, January 2016
- Rahbar, Kambiz; Bode, Axel; Weckesser, Matthias
- Clinical Nuclear Medicine, Vol. 41, Issue 7
Imaging Tumors with an Albumin-Binding Fab, a Novel Tumor-Targeting Agent
journal, January 2007
- Dennis, Mark S.; Jin, Hongkui; Dugger, Debra
- Cancer Research, Vol. 67, Issue 1
Radiolabeled Small-Molecule Ligands for Prostate-Specific Membrane Antigen: In vivo Imaging in Experimental Models of Prostate Cancer
journal, June 2005
- Foss, Catherine A.; Mease, Ronnie C.; Fan, Hong
- Clinical Cancer Research, Vol. 11, Issue 11
Radioimmunotherapy of Prostate Cancer Using 90Y- and 177Lu-Labeled J591 Monoclonal Antibodies: Effect of Multiple Treatments on Myelotoxicity
journal, October 2005
- Vallabhajosula, Shankar; Goldsmith, Stanley J.; Kostakoglu, Lale
- Clinical Cancer Research, Vol. 11, Issue 19
11C-MCG: Synthesis, Uptake Selectivity, and Primate PET of a Probe for Glutamate Carboxypeptidase II (NAALADase)
journal, April 2002
- Pomper, Martin G.; Musachio, John L.; Zhang, Jiazhong
- Molecular Imaging, Vol. 1, Issue 2
Early side effects and first results of radioligand therapy with 177Lu-DKFZ-617 PSMA of castrate-resistant metastatic prostate cancer: a two-centre study
journal, June 2015
- Ahmadzadehfar, Hojjat; Rahbar, Kambiz; Kürpig, Stefan
- EJNMMI Research, Vol. 5, Issue 1
Phase I Trial of 177 Lutetium-Labeled J591, a Monoclonal Antibody to Prostate-Specific Membrane Antigen, in Patients With Androgen-Independent Prostate Cancer
journal, July 2005
- Bander, Neil H.; Milowsky, Matthew I.; Nanus, David M.
- Journal of Clinical Oncology, Vol. 23, Issue 21
Vascular Targeted Therapy With Anti–Prostate-Specific Membrane Antigen Monoclonal Antibody J591 in Advanced Solid Tumors
journal, February 2007
- Milowsky, Matthew I.; Nanus, David M.; Kostakoglu, Lale
- Journal of Clinical Oncology, Vol. 25, Issue 5
High-affinity Near-infrared Fluorescent Small-molecule Contrast Agents for In Vivo Imaging of Prostate-specific Membrane Antigen
journal, October 2005
- Humblet, Valerie; Lapidus, Rena; Williams, Larry R.
- Molecular Imaging, Vol. 4, Issue 4
Ins and outs of clinical trials with peptide-based vaccines
journal, January 2002
- Salit, Rachel, B.
- Frontiers in Bioscience, Vol. 7, Issue 1-3
Assessment of an 18 F-Labeled Phosphoramidate Peptidomimetic as a New Prostate-Specific Membrane Antigen–Targeted Imaging Agent for Prostate Cancer
journal, November 2009
- Lapi, Suzanne E.; Wahnishe, Hilla; Pham, David
- Journal of Nuclear Medicine, Vol. 50, Issue 12
DOTA Conjugate with an Albumin-Binding Entity Enables the First Folic Acid–Targeted 177 Lu-Radionuclide Tumor Therapy in Mice
journal, December 2012
- Müller, Cristina; Struthers, Harriet; Winiger, Christian
- Journal of Nuclear Medicine, Vol. 54, Issue 1
PMPA for Nephroprotection in PSMA-Targeted Radionuclide Therapy of Prostate Cancer
journal, January 2015
- Kratochwil, Clemens; Giesel, Frederik L.; Leotta, Karin
- Journal of Nuclear Medicine, Vol. 56, Issue 2
68Ga- and 177Lu-Labeled PSMA I&T: Optimization of a PSMA-Targeted Theranostic Concept and First Proof-of-Concept Human Studies
journal, June 2015
- Weineisen, M.; Schottelius, M.; Simecek, J.
- Journal of Nuclear Medicine, Vol. 56, Issue 8
177Lu-Labeled Prostate-Specific Membrane Antigen Radioligand Therapy of Metastatic Castration-Resistant Prostate Cancer: Safety and Efficacy
journal, January 2016
- Baum, R. P.; Kulkarni, H. R.; Schuchardt, C.
- Journal of Nuclear Medicine, Vol. 57, Issue 7
Perspective on 177Lu-PSMA Therapy for Metastatic Castration-Resistant Prostate Cancer
journal, February 2016
- Kwekkeboom, Dik
- Journal of Nuclear Medicine, Vol. 57, Issue 7
(2S)-2-(3-(1-Carboxy-5-(4-211At-Astatobenzamido)Pentyl)Ureido)-Pentanedioic Acid for PSMA-Targeted -Particle Radiopharmaceutical Therapy
journal, May 2016
- Kiess, A. P.; Minn, I.; Vaidyanathan, G.
- Journal of Nuclear Medicine, Vol. 57, Issue 10
Detection of prostate-specific membrane antigen on HUVECs in response to breast tumor-conditioned medium
journal, May 2011
- Berkman,
- International Journal of Oncology, Vol. 38, Issue 5
Targeted photodynamic therapy for prostate cancer: Inducing apoptosis via activation of the caspase-8/-3 cascade pathway
journal, March 2010
- Berkman,
- International Journal of Oncology, Vol. 36, Issue 4
Towards Personalized Treatment of Prostate Cancer: PSMA I&T, a Promising Prostate-Specific Membrane Antigen-Targeted Theranostic Agent
journal, January 2016
- Chatalic, Kristell L. S.; Heskamp, Sandra; Konijnenberg, Mark
- Theranostics, Vol. 6, Issue 6
Works referencing / citing this record:
177Lu-labeled low-molecular-weight agents for PSMA-targeted radiopharmaceutical therapy
journal, August 2019
- Banerjee, Sangeeta Ray; Kumar, Vivek; Lisok, Ala
- European Journal of Nuclear Medicine and Molecular Imaging, Vol. 46, Issue 12
177Lu-DOTA-HYNIC-Lys(Nal)-Urea-Glu: synthesis and assessment of the ability to target the prostate specific membrane antigen
journal, October 2018
- Hernández-Jiménez, Tania; Ferro-Flores, Guillermina; Ocampo-García, Blanca
- Journal of Radioanalytical and Nuclear Chemistry, Vol. 318, Issue 3
Synthesis and preclinical evaluation of the 99mTc-/177Lu-CXCR4-L theranostic pair for in vivo chemokine-4 receptor-specific targeting
journal, February 2020
- Ávila-Sánchez, Marcela; Ferro-Flores, Guillermina; Jiménez-Mancilla, Nallely
- Journal of Radioanalytical and Nuclear Chemistry, Vol. 324, Issue 1
Preclinical Dosimetry, Imaging, and Targeted Radionuclide Therapy Studies of Lu-177-Labeled Albumin-Binding, PSMA-Targeted CTT1403
journal, July 2019
- Ling, Xiaoxi; Latoche, Joseph D.; Choy, Cindy J.
- Molecular Imaging and Biology, Vol. 22, Issue 2
Alpha-PET for Prostate Cancer: Preclinical investigation using 149Tb-PSMA-617
journal, November 2019
- Umbricht, Christoph A.; Köster, Ulli; Bernhardt, Peter
- Scientific Reports, Vol. 9, Issue 1
Synthesis and in vitro and in vivo evaluation of urea-based PSMA inhibitors with increased lipophilicity
journal, August 2018
- Wirtz, Martina; Schmidt, Alexander; Schottelius, Margret
- EJNMMI Research, Vol. 8, Issue 1
The Future of PSMA-Targeted Radionuclide Therapy: An Overview of Recent Preclinical Research
journal, October 2019
- Ruigrok, Eline A. M.; van Weerden, Wytske M.; Nonnekens, Julie
- Pharmaceutics, Vol. 11, Issue 11
Alpha-PET for Prostate Cancer: Preclinical investigation using 149Tb-PSMA-617
text, January 2019
- Umbricht, Christoph A.; Köster, Ulli; Bernhardt, Peter
- ETH Zurich
66Ga: A Novelty or a Valuable Preclinical Screening Tool for the Design of Targeted Radiopharmaceuticals?
journal, October 2018
- Amor-Coarasa, Alejandro; Kelly, James; Ponnala, Shashikanth
- Molecules, Vol. 23, Issue 10
Development of a new class of PSMA radioligands comprising ibuprofen as an albumin-binding entity
journal, January 2020
- Deberle, Luisa M.; Benešová, Martina; Umbricht, Christoph A.
- Theranostics, Vol. 10, Issue 4
Targeted alpha therapy in a systemic mouse model of prostate cancer - a feasibility study
journal, January 2020
- Stuparu, Andreea D.; Meyer, Catherine A. L.; Evans-Axelsson, Susan L.
- Theranostics, Vol. 10, Issue 6