skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Evolution of Electrogenic Ammonium Transporters (AMTs)

Abstract

The ammonium transporter gene family consists of three main clades, AMT, MEP, and Rh. The evolutionary history of the AMT/MEP/Rh gene family is characterized by multiple horizontal gene transfer events, gene family expansion and contraction, and gene loss; thus the gene tree for this family of transporters is unlike the organismal tree. The genomes of angiosperms contain genes for both electrogenic and electroneutral ammonium transporters, but it is not clear how far back in the land plant lineage electrogenic ammonium transporters occur. Here, we place Marchantia polymorpha ammonium transporters in the AMT/MEP/Rh phylogeny and we show that AMTs from the liverwort M. polymorpha are electrogenic. This information suggests that electrogenic ammonium transport evolved at least as early as the divergence of bryophytes in the land plant lineage.

Authors:
 [1];  [2]
  1. St. Catherine Univ., St.Paul, MN (United States). Biology Dept.
  2. Univ. of Minnesota, St. Paul, MN (United States). Dept. of Plant Biology
Publication Date:
Research Org.:
St. Catherine Univ., St.Paul, MN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1393413
Grant/Contract Number:  
FG02-10ER15886; FG02-07ER15886
Resource Type:
Accepted Manuscript
Journal Name:
Frontiers in Plant Science
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 1664-462X
Publisher:
Frontiers Research Foundation
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ammonium transporters; electrophysiology; Marchantia polymorpha; phylogeny; electrogenicity

Citation Formats

McDonald, Tami R., and Ward, John M. Evolution of Electrogenic Ammonium Transporters (AMTs). United States: N. p., 2016. Web. https://doi.org/10.3389/fpls.2016.00352.
McDonald, Tami R., & Ward, John M. Evolution of Electrogenic Ammonium Transporters (AMTs). United States. https://doi.org/10.3389/fpls.2016.00352
McDonald, Tami R., and Ward, John M. Thu . "Evolution of Electrogenic Ammonium Transporters (AMTs)". United States. https://doi.org/10.3389/fpls.2016.00352. https://www.osti.gov/servlets/purl/1393413.
@article{osti_1393413,
title = {Evolution of Electrogenic Ammonium Transporters (AMTs)},
author = {McDonald, Tami R. and Ward, John M.},
abstractNote = {The ammonium transporter gene family consists of three main clades, AMT, MEP, and Rh. The evolutionary history of the AMT/MEP/Rh gene family is characterized by multiple horizontal gene transfer events, gene family expansion and contraction, and gene loss; thus the gene tree for this family of transporters is unlike the organismal tree. The genomes of angiosperms contain genes for both electrogenic and electroneutral ammonium transporters, but it is not clear how far back in the land plant lineage electrogenic ammonium transporters occur. Here, we place Marchantia polymorpha ammonium transporters in the AMT/MEP/Rh phylogeny and we show that AMTs from the liverwort M. polymorpha are electrogenic. This information suggests that electrogenic ammonium transport evolved at least as early as the divergence of bryophytes in the land plant lineage.},
doi = {10.3389/fpls.2016.00352},
journal = {Frontiers in Plant Science},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Citation Metrics:
Cited by: 9 works
Citation information provided by
Web of Science

Save / Share:

Works referenced in this record:

RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies
journal, January 2014


Different Transport Mechanisms in Plant and Human AMT/Rh-type Ammonium Transporters
journal, January 2006

  • Mayer, Maria; Schaaf, Gabriel; Mouro, Isabelle
  • Journal of General Physiology, Vol. 127, Issue 2
  • DOI: 10.1085/jgp.200509369

Biological gas channels for NH3 and CO2: evidence that Rh (Rhesus) proteins are CO2 channels
journal, March 2006


Crystal structure of the archaeal ammonium transporter Amt-1 from Archaeoglobus fulgidus
journal, October 2005

  • Andrade, S. L. A.; Dickmanns, A.; Ficner, R.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 42
  • DOI: 10.1073/pnas.0506254102

Ammonium ion transport by the AMT/Rh homologue LeAMT1;1
journal, May 2006

  • Mayer, Maria; Dynowski, Marek; Ludewig, Uwe
  • Biochemical Journal, Vol. 396, Issue 3
  • DOI: 10.1042/BJ20060051

An Improved General Amino Acid Replacement Matrix
journal, April 2008


Multiple Horizontal Gene Transfers of Ammonium Transporters/Ammonia Permeases from Prokaryotes to Eukaryotes: Toward a New Functional and Evolutionary Classification
journal, June 2011

  • McDonald, T. R.; Dietrich, F. S.; Lutzoni, F.
  • Molecular Biology and Evolution, Vol. 29, Issue 1
  • DOI: 10.1093/molbev/msr123

Uncoupling of Ionic Currents from Substrate Transport in the Plant Ammonium Transporter At AMT1;2
journal, March 2014

  • Neuhäuser, Benjamin; Ludewig, Uwe
  • Journal of Biological Chemistry, Vol. 289, Issue 17
  • DOI: 10.1074/jbc.C114.552802

Arabidopsis Sucrose Transporter AtSUC9. High-Affinity Transport Activity, Intragenic Control of Expression, and Early Flowering Mutant Phenotype
journal, November 2006

  • Sivitz, Alicia B.; Reinders, Anke; Johnson, Meghan E.
  • Plant Physiology, Vol. 143, Issue 1
  • DOI: 10.1104/pp.106.089003

Amt/MEP/Rh proteins conduct ammonia
journal, November 2005


Structural and mechanistic aspects of Amt/Rh proteins
journal, June 2007

  • Javelle, Arnaud; Lupo, Domenico; Li, Xiao-Dan
  • Journal of Structural Biology, Vol. 158, Issue 3
  • DOI: 10.1016/j.jsb.2007.01.004

Dendroscope 3: An Interactive Tool for Rooted Phylogenetic Trees and Networks
journal, September 2012


Creating the CIPRES Science Gateway for inference of large phylogenetic trees
conference, November 2010

  • Miller, Mark A.; Pfeiffer, Wayne; Schwartz, Terri
  • 2010 Gateway Computing Environments Workshop (GCE)
  • DOI: 10.1109/GCE.2010.5676129

Ammonia acquisition in enteric bacteria: Physiological role of the ammonium/methylammonium transport B (AmtB) protein
journal, June 1998

  • Soupene, E.; He, L.; Yan, D.
  • Proceedings of the National Academy of Sciences, Vol. 95, Issue 12
  • DOI: 10.1073/pnas.95.12.7030

The rice OsAMT1;1 is a proton-independent feedback regulated ammonium transporter
journal, November 2014


The 1.3-A resolution structure of Nitrosomonas europaea Rh50 and mechanistic implications for NH3 transport by Rhesus family proteins
journal, November 2007

  • Lupo, D.; Li, X. -D.; Durand, A.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 49
  • DOI: 10.1073/pnas.0706563104

jModelTest: Phylogenetic Model Averaging
journal, April 2008


The mechanism of ammonia transport based on the crystal structure of AmtB of Escherichia coli
journal, November 2004

  • Zheng, L.; Kostrewa, D.; Berneche, S.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 49
  • DOI: 10.1073/pnas.0406475101

Pv AMT1;1, a Highly Selective Ammonium Transporter That Functions as H + /NH 4 + Symporter
journal, July 2011

  • Ortiz-Ramirez, Carlos; Mora, Silvia I.; Trejo, Jorge
  • Journal of Biological Chemistry, Vol. 286, Issue 36
  • DOI: 10.1074/jbc.M111.261693

Structure of the Nitrosomonas europaea Rh protein
journal, November 2007

  • Li, X.; Jayachandran, S.; Nguyen, H. -H. T.
  • Proceedings of the National Academy of Sciences, Vol. 104, Issue 49
  • DOI: 10.1073/pnas.0709710104

Uniport of NH by the Root Hair Plasma Membrane Ammonium Transporter LeAMT1;1
journal, January 2002

  • Ludewig, Uwe; von Wirén, Nico; Frommer, Wolf B.
  • Journal of Biological Chemistry, Vol. 277, Issue 16
  • DOI: 10.1074/jbc.M200739200

Characterization of Arabidopsis AtAMT2, a novel ammonium transporter in plants
journal, February 2000


In vivo functional characterization of the Escherichia coli ammonium channel AmtB: evidence for metabolic coupling of AmtB to glutamine synthetase
journal, August 2005

  • Javelle, Arnaud; Thomas, Gavin; Marini, Anne-Marie
  • Biochemical Journal, Vol. 390, Issue 1
  • DOI: 10.1042/BJ20042094

Antennal-Expressed Ammonium Transporters in the Malaria Vector Mosquito Anopheles gambiae
journal, October 2014


Function of human Rh based on structure of RhCG at 2.1 A
journal, May 2010

  • Gruswitz, F.; Chaudhary, S.; Ho, J. D.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 21
  • DOI: 10.1073/pnas.1003587107

ProtTest: selection of best-fit models of protein evolution
journal, January 2005


Mechanism of Ammonia Transport by Amt/MEP/Rh: Structure of AmtB at 1.35 A
journal, September 2004


Evolutionary conservation and diversification of Rh family genes and proteins
journal, October 2005

  • Huang, C. -H.; Peng, J.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 43
  • DOI: 10.1073/pnas.0507886102

Channel-like NH 3 flux by ammonium transporter AtAMT2
journal, July 2009


Switching substrate specificity of AMT/MEP/ Rh proteins
journal, October 2014


The Dionaea muscipula Ammonium Channel DmAMT1 Provides NH4+ Uptake Associated with Venus Flytrap’s Prey Digestion
journal, September 2013


Transport Activity of Rice Sucrose Transporters OsSUT1 and OsSUT5
journal, December 2009

  • Sun, Y.; Reinders, A.; LaFleur, K. R.
  • Plant and Cell Physiology, Vol. 51, Issue 1
  • DOI: 10.1093/pcp/pcp172

The Rh (Rhesus) blood group polypeptides are related to NH4+ transporters
journal, December 1997

  • Marini, Anne-Marie; Urrestarazu, Antonio; Beauwens, Renaud
  • Trends in Biochemical Sciences, Vol. 22, Issue 12
  • DOI: 10.1016/S0968-0004(97)01132-8

Cloning and expression of the MEP1 gene encoding an ammonium transporter in Saccharomyces cerevisiae.
journal, August 1994


Identification of a high affinity NH4+ transporter from plants.
journal, August 1994


    Works referencing / citing this record:

    Isolation and characterization of a high-affinity ammonium transporter ApAMT1;1 in alligatorweed
    journal, September 2019


    The lipid environment determines the activity of the Escherichia coli ammonium transporter AmtB
    journal, September 2018

    • Mirandela, Gaëtan Dias; Tamburrino, Giulia; Hoskisson, Paul A.
    • The FASEB Journal, Vol. 33, Issue 2
    • DOI: 10.1096/fj.201800782r

    Nitrogen isotope signature evidences ammonium deprotonation as a common transport mechanism for the AMT-Mep-Rh protein superfamily
    journal, September 2018

    • Ariz, Idoia; Boeckstaens, Mélanie; Gouveia, Catarina
    • Science Advances, Vol. 4, Issue 9
    • DOI: 10.1126/sciadv.aar3599

    Ammonia excretion in aquatic invertebrates: new insights and questions
    journal, January 2018

    • Weihrauch, Dirk; Joseph, Garett; Allen, Patrick
    • The Journal of Experimental Biology, Vol. 221, Issue 2
    • DOI: 10.1242/jeb.169219