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Title: A Genetic Screen for Mutations Affecting Cell Division in the Arabidopsis thaliana Embryo Identifies Seven Loci Required for Cytokinesis

Abstract

Cytokinesis in plants involves the formation of unique cellular structures such as the phragmoplast and the cell plate, both of which are required to divide the cell after nuclear division. In order to isolate genes that are involved in de novo cell wall formation, we performed a large-scale, microscope-based screen for Arabidopsis mutants that severely impair cytokinesis in the embryo. We recovered 35 mutations that form abnormally enlarged cells with multiple, often polyploid nuclei and incomplete cell walls. These mutants represent seven genes, four of which have previously been implicated in phragmoplast or cell plate function. Mutations in two loci show strongly reduced transmission through the haploid gametophytic generation. Molecular cloning of both corresponding genes reveals that one is represented by hypomorphic alleles of the kinesin-5 gene RADIALLY SWOLLEN 7 (homologous to tobacco kinesin-related protein TKRP125), and that the other gene corresponds to the Arabidopsis FUSED ortholog TWO-IN-ONE (originally identified based on its function in pollen development). No mutations that completely abolish the formation of cross walls in diploid cells were found. Lastly, our results support the idea that cytokinesis in the diploid and haploid generations involve similar mechanisms.

Authors:
 [1];  [1];  [2];  [1];  [2]
  1. Carnegie Inst., Stanford, CA (United States). Dept. of Plant Biology; Stanford Univ., CA (United States). Dept. of Biological Sciences
  2. Carnegie Inst., Stanford, CA (United States). Dept. of Plant Biology
Publication Date:
Research Org.:
Carnegie Inst. of Washington, Stanford, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1393410
Grant/Contract Number:  
FG02-03ER20133; FG02-94ER20133
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 11; Journal Issue: 1; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; Embryos; Seedlings; Cytokinesis; Arabidopsis thailana; Alleles; cell cycle and cell division; Pollen; Mutation

Citation Formats

Gillmor, C. Stewart, Roeder, Adrienne H. K., Sieber, Patrick, Somerville, Chris, and Lukowitz, Wolfgang. A Genetic Screen for Mutations Affecting Cell Division in the Arabidopsis thaliana Embryo Identifies Seven Loci Required for Cytokinesis. United States: N. p., 2016. Web. doi:10.1371/journal.pone.0146492.
Gillmor, C. Stewart, Roeder, Adrienne H. K., Sieber, Patrick, Somerville, Chris, & Lukowitz, Wolfgang. A Genetic Screen for Mutations Affecting Cell Division in the Arabidopsis thaliana Embryo Identifies Seven Loci Required for Cytokinesis. United States. doi:10.1371/journal.pone.0146492.
Gillmor, C. Stewart, Roeder, Adrienne H. K., Sieber, Patrick, Somerville, Chris, and Lukowitz, Wolfgang. Fri . "A Genetic Screen for Mutations Affecting Cell Division in the Arabidopsis thaliana Embryo Identifies Seven Loci Required for Cytokinesis". United States. doi:10.1371/journal.pone.0146492. https://www.osti.gov/servlets/purl/1393410.
@article{osti_1393410,
title = {A Genetic Screen for Mutations Affecting Cell Division in the Arabidopsis thaliana Embryo Identifies Seven Loci Required for Cytokinesis},
author = {Gillmor, C. Stewart and Roeder, Adrienne H. K. and Sieber, Patrick and Somerville, Chris and Lukowitz, Wolfgang},
abstractNote = {Cytokinesis in plants involves the formation of unique cellular structures such as the phragmoplast and the cell plate, both of which are required to divide the cell after nuclear division. In order to isolate genes that are involved in de novo cell wall formation, we performed a large-scale, microscope-based screen for Arabidopsis mutants that severely impair cytokinesis in the embryo. We recovered 35 mutations that form abnormally enlarged cells with multiple, often polyploid nuclei and incomplete cell walls. These mutants represent seven genes, four of which have previously been implicated in phragmoplast or cell plate function. Mutations in two loci show strongly reduced transmission through the haploid gametophytic generation. Molecular cloning of both corresponding genes reveals that one is represented by hypomorphic alleles of the kinesin-5 gene RADIALLY SWOLLEN 7 (homologous to tobacco kinesin-related protein TKRP125), and that the other gene corresponds to the Arabidopsis FUSED ortholog TWO-IN-ONE (originally identified based on its function in pollen development). No mutations that completely abolish the formation of cross walls in diploid cells were found. Lastly, our results support the idea that cytokinesis in the diploid and haploid generations involve similar mechanisms.},
doi = {10.1371/journal.pone.0146492},
journal = {PLoS ONE},
number = 1,
volume = 11,
place = {United States},
year = {2016},
month = {1}
}

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Cited by: 6 works
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