Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking
Abstract
A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.
- Publication Date:
- Research Org.:
- Univ. of Tennessee, Knoxville, TN (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); National Institutes of Health (NIH)
- OSTI Identifier:
- 1368434
- Alternate Identifier(s):
- OSTI ID: 1393145
- Grant/Contract Number:
- NA0001983; GM112496
- Resource Type:
- Published Article
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Name: PLoS ONE Journal Volume: 12 Journal Issue: 7; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science (PLoS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E., and Chen, ed., Chin-Tu. Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking. United States: N. p., 2017.
Web. doi:10.1371/journal.pone.0180503.
Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E., & Chen, ed., Chin-Tu. Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking. United States. https://doi.org/10.1371/journal.pone.0180503
Langford, Seth T., Wiggins, Cody S., Santos, Roque, Hauser, Melinda, Becker, Jeffrey M., Ruggles, Arthur E., and Chen, ed., Chin-Tu. Thu .
"Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking". United States. https://doi.org/10.1371/journal.pone.0180503.
@article{osti_1368434,
title = {Three-dimensional spatiotemporal tracking of fluorine-18 radiolabeled yeast cells via positron emission particle tracking},
author = {Langford, Seth T. and Wiggins, Cody S. and Santos, Roque and Hauser, Melinda and Becker, Jeffrey M. and Ruggles, Arthur E. and Chen, ed., Chin-Tu},
abstractNote = {A method for Positron Emission Particle Tracking (PEPT) based on optical feature point identification techniques is demonstrated for use in low activity tracking experiments. Furthermore, a population of yeast cells of approximately 125,000 members is activated to roughly 55 Bq/cell by 18F uptake. An in vitro particle tracking experiment is performed with nearly 20 of these cells after decay to 32 Bq/cell. These cells are successfully identified and tracked simultaneously in this experiment. Our work extends the applicability of PEPT as a cell tracking method by allowing a number of cells to be tracked together, and demonstrating tracking for very low activity tracers.},
doi = {10.1371/journal.pone.0180503},
journal = {PLoS ONE},
number = 7,
volume = 12,
place = {United States},
year = {Thu Jul 06 00:00:00 EDT 2017},
month = {Thu Jul 06 00:00:00 EDT 2017}
}
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https://doi.org/10.1371/journal.pone.0180503
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