Quantitative Reflection Imaging for the Morphology and Dynamics of Live Aplysia californica Pedal Ganglion Neurons Cultured on Nanostructured Plasmonic Crystals
Abstract
We describe a reflection imaging system that consists of a plasmonic crystal, a common laboratory microscope, and band-pass filters for use in the quantitative imaging and in situ monitoring of live cells and their substrate interactions. Surface plasmon resonance (SPR) provides a highly sensitive method to monitor changes in physicochemical properties occurring at metal-dielectric interfaces. Polyelectrolyte thin films deposited using the layer-by-layer (LBL) self-assembly method provide a reference system for calibrating the reflection contrast changes that occur when the polyelectrolyte film thickness changes and provide insight into the optical responses that originate from the multiple plasmonic features supported by this imaging system. Finitedifference time-domain (FDTD) simulations of the optical responses measured experimentally from the polyelectrolyte reference system are used to provide a calibration of the optical system for subsequent use in quantitative studies investigating live cell dynamics in cultures supported on a plasmonic crystal substrate. Live Aplysia californica pedal ganglion neurons cultured in artificial seawater were used as a model system through which to explore the utility of this plasmonic imaging technique. Here, the morphology of cellular peripheral structures ≲80 nm in thickness were quantitatively analyzed, and the dynamics of their trypsin-induced surface detachment were visualized. These results illustrate themore »
- Authors:
-
[2];
[3];
- Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Materials Science and Engineering
- Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry
- Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Chemistry, and Beckman Inst. for Advanced Science and Technology
- Univ. of Illinois at Urbana-Champaign, IL (United States). Dept. of Materials Science and Engineering, and Dept. of Chemistry
- Publication Date:
- Research Org.:
- Energy Frontier Research Centers (EFRC) (United States). Light-Material Interactions in Energy Conversion (LMI)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1388238
- Grant/Contract Number:
- SC0001293
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Langmuir
- Additional Journal Information:
- Journal Volume: 33; Journal Issue: 35; Related Information: LMI partners with California Institute of Technology (lead); Harvard University; University of Illinois, Urbana-Champaign; Lawrence Berkeley National Laboratory; Journal ID: ISSN 0743-7463
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 47 OTHER INSTRUMENTATION; solar (photovoltaic); solid state lighting; phonons; thermal conductivity; electrodes - solar; materials and chemistry by design; optics; synthesis (novel materials); synthesis (self-assembly)
Citation Formats
Kang, Somi, Badea, Adina, Rubakhin, Stanislav S., Sweedler, Jonathan V., Rogers, John A., and Nuzzo, Ralph G. Quantitative Reflection Imaging for the Morphology and Dynamics of Live Aplysia californica Pedal Ganglion Neurons Cultured on Nanostructured Plasmonic Crystals. United States: N. p., 2017.
Web. doi:10.1021/acs.langmuir.6b04454.
Kang, Somi, Badea, Adina, Rubakhin, Stanislav S., Sweedler, Jonathan V., Rogers, John A., & Nuzzo, Ralph G. Quantitative Reflection Imaging for the Morphology and Dynamics of Live Aplysia californica Pedal Ganglion Neurons Cultured on Nanostructured Plasmonic Crystals. United States. https://doi.org/10.1021/acs.langmuir.6b04454
Kang, Somi, Badea, Adina, Rubakhin, Stanislav S., Sweedler, Jonathan V., Rogers, John A., and Nuzzo, Ralph G. Tue .
"Quantitative Reflection Imaging for the Morphology and Dynamics of Live Aplysia californica Pedal Ganglion Neurons Cultured on Nanostructured Plasmonic Crystals". United States. https://doi.org/10.1021/acs.langmuir.6b04454. https://www.osti.gov/servlets/purl/1388238.
@article{osti_1388238,
title = {Quantitative Reflection Imaging for the Morphology and Dynamics of Live Aplysia californica Pedal Ganglion Neurons Cultured on Nanostructured Plasmonic Crystals},
author = {Kang, Somi and Badea, Adina and Rubakhin, Stanislav S. and Sweedler, Jonathan V. and Rogers, John A. and Nuzzo, Ralph G.},
abstractNote = {We describe a reflection imaging system that consists of a plasmonic crystal, a common laboratory microscope, and band-pass filters for use in the quantitative imaging and in situ monitoring of live cells and their substrate interactions. Surface plasmon resonance (SPR) provides a highly sensitive method to monitor changes in physicochemical properties occurring at metal-dielectric interfaces. Polyelectrolyte thin films deposited using the layer-by-layer (LBL) self-assembly method provide a reference system for calibrating the reflection contrast changes that occur when the polyelectrolyte film thickness changes and provide insight into the optical responses that originate from the multiple plasmonic features supported by this imaging system. Finitedifference time-domain (FDTD) simulations of the optical responses measured experimentally from the polyelectrolyte reference system are used to provide a calibration of the optical system for subsequent use in quantitative studies investigating live cell dynamics in cultures supported on a plasmonic crystal substrate. Live Aplysia californica pedal ganglion neurons cultured in artificial seawater were used as a model system through which to explore the utility of this plasmonic imaging technique. Here, the morphology of cellular peripheral structures ≲80 nm in thickness were quantitatively analyzed, and the dynamics of their trypsin-induced surface detachment were visualized. These results illustrate the capacities of this system for use in investigations of the dynamics of ultrathin cellular structures within complex bioanalytical environments.},
doi = {10.1021/acs.langmuir.6b04454},
journal = {Langmuir},
number = 35,
volume = 33,
place = {United States},
year = {Tue Mar 07 00:00:00 EST 2017},
month = {Tue Mar 07 00:00:00 EST 2017}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 3 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Evolving Mechanisms of Morphogenesis: on the Interplay between Differential Adhesion and Cell Differentiation
journal, April 2000
- Hogeweg, P.
- Journal of Theoretical Biology, Vol. 203, Issue 4
Local force and geometry sensing regulate cell functions
journal, February 2006
- Vogel, Viola; Sheetz, Michael
- Nature Reviews Molecular Cell Biology, Vol. 7, Issue 4
Cell and molecular mechanics of biological materials
journal, November 2003
- Bao, G.; Suresh, S.
- Nature Materials, Vol. 2, Issue 11
Cell surface mechanics and the control of cell shape, tissue patterns and morphogenesis
journal, August 2007
- Lecuit, Thomas; Lenne, Pierre-François
- Nature Reviews Molecular Cell Biology, Vol. 8, Issue 8
Preface
book, January 2012
- Michael Conn, P.
- Imaging and Spectroscopic Analysis of Living Cells - Optical and Spectroscopic Techniques
Use of quantum dots for live cell imaging
journal, October 2004
- Jaiswal, Jyoti K.; Goldman, Ellen R.; Mattoussi, Hedi
- Nature Methods, Vol. 1, Issue 1
Light Microscopy Techniques for Live Cell Imaging
journal, April 2003
- Stephens, D. J.
- Science, Vol. 300, Issue 5616
Exploring Neural Cell Dynamics with Digital Holographic Microscopy
journal, July 2013
- Marquet, P.; Depeursinge, C.; Magistretti, P. J.
- Annual Review of Biomedical Engineering, Vol. 15, Issue 1
Cytoskeleton dynamics control the first asymmetric cell division in Arabidopsis zygote
journal, November 2016
- Kimata, Yusuke; Higaki, Takumi; Kawashima, Tomokazu
- Proceedings of the National Academy of Sciences, Vol. 113, Issue 49
Using Cell-substrate Impedance and Live Cell Imaging to Measure Real-time Changes in Cellular Adhesion and De-adhesion Induced by Matrix Modification
journal, January 2015
- Rees, Martin D.; Thomas, Shane R.
- Journal of Visualized Experiments, Issue 96
Digital holographic microscopy: a noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy
journal, January 2005
- Marquet, Pierre; Rappaz, Benjamin; Magistretti, Pierre J.
- Optics Letters, Vol. 30, Issue 5
Raman Microscopy and Associated Techniques for Label-Free Imaging of Cancer Tissue
journal, June 2015
- Downes, Andrew
- Applied Spectroscopy Reviews, Vol. 50, Issue 8
Live-cell microscopy – tips and tools
journal, March 2009
- Frigault, Melanie M.; Lacoste, Judith; Swift, Jody L.
- Journal of Cell Science, Vol. 122, Issue 6
Influence of extracellular pH on the cytotoxicity, cellular accumulation, and DNA interaction of novel pH-sensitive 2-aminoalcoholatoplatinum(II) complexes
journal, January 2013
- Valiahdi, Seied Mojtaba; Egger, Alexander E.; Miklos, Walter
- JBIC Journal of Biological Inorganic Chemistry, Vol. 18, Issue 2
Mechanisms and Consequences of pH-Mediated Cell Regulation
journal, January 1986
- Busa, W.
- Annual Review of Physiology, Vol. 48, Issue 1
Oxidative stress is involved in heat-induced cell death in Saccharomyces cerevisiae.
journal, May 1996
- Davidson, J. F.; Whyte, B.; Bissinger, P. H.
- Proceedings of the National Academy of Sciences, Vol. 93, Issue 10
Mechanisms of Cell Volume Regulation in Hypo-osmolality
journal, July 2006
- Pasantes-Morales, Herminia; Lezama, Ruth A.; Ramos-Mandujano, Gerardo
- The American Journal of Medicine, Vol. 119, Issue 7
Long-term two-photon fluorescence imaging of mammalian embryos without compromising viability
journal, August 1999
- Squirrell, Jayne M.; Wokosin, David L.; White, John G.
- Nature Biotechnology, Vol. 17, Issue 8
Fluorescence resonance energy transfer (FRET) microscopy imaging of live cell protein localizations
journal, March 2003
- Sekar, Rajesh Babu; Periasamy, Ammasi
- The Journal of Cell Biology, Vol. 160, Issue 5
Quantum Dots for Live Cells, in Vivo Imaging, and Diagnostics
journal, January 2005
- Michalet, X.
- Science, Vol. 307, Issue 5709
Development and Use of Fluorescent Protein Markers in Living Cells
journal, April 2003
- Lippincott-Schwartz, J.
- Science, Vol. 300, Issue 5616
Preparation of plants for developmental and cellular imaging by light-sheet microscopy
journal, July 2015
- Ovečka, Miroslav; Vaškebová, Lenka; Komis, George
- Nature Protocols, Vol. 10, Issue 8
Controlled light-exposure microscopy reduces photobleaching and phototoxicity in fluorescence live-cell imaging
journal, January 2007
- Hoebe, R. A.; Van Oven, C. H.; Gadella, T. W. J.
- Nature Biotechnology, Vol. 25, Issue 2
A guide to choosing fluorescent proteins
journal, November 2005
- Shaner, Nathan C.; Steinbach, Paul A.; Tsien, Roger Y.
- Nature Methods, Vol. 2, Issue 12
Quantitative optical phase microscopy
journal, January 1998
- Barty, A.; Nugent, K. A.; Paganin, D.
- Optics Letters, Vol. 23, Issue 11
In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro
journal, February 2007
- Liang, Chun-Chi; Park, Ann Y.; Guan, Jun-Lin
- Nature Protocols, Vol. 2, Issue 2
Video-enhanced contrast, differential interference contrast (AVEC-DIC) microscopy: A new method capable of analyzing microtubule-related motility in the reticulopodial network of allogromia laticollaris
journal, January 1981
- Allen, Robert Day; Allen, Nina Strömgren; Travis, Jeffrey L.
- Cell Motility, Vol. 1, Issue 3
Visualizing unstained neurons in living brain slices by infrared DIC-videomicroscopy
journal, December 1990
- Dodt, Hans-Ulrich; Zieglgänsberger, Walter
- Brain Research, Vol. 537, Issue 1-2
3D Microperiodic Hydrogel Scaffolds for Robust Neuronal Cultures
journal, November 2010
- Hanson Shepherd, Jennifer N.; Parker, Sara T.; Shepherd, Robert F.
- Advanced Functional Materials, Vol. 21, Issue 1
Spinning-disk Confocal Microscopy — A Cutting-Edge Tool for Imaging of Membrane Traffic
journal, January 2002
- Nakano, Akihiko
- Cell Structure and Function, Vol. 27, Issue 5
Long-term multiple color imaging of live cells using quantum dot bioconjugates
journal, December 2002
- Jaiswal, Jyoti K.; Mattoussi, Hedi; Mauro, J. Matthew
- Nature Biotechnology, Vol. 21, Issue 1
Fundamental Limits in Confocal Microscopy
book, January 1990
- Pawley, James
- Handbook of Biological Confocal Microscopy
Spatial light interference microscopy (SLIM)
journal, January 2011
- Wang, Zhuo; Millet, Larry; Mir, Mustafa
- Optics Express, Vol. 19, Issue 2
Drug-Induced Changes of Cytoskeletal Structure and Mechanics in Fibroblasts: An Atomic Force Microscopy Study
journal, January 2000
- Rotsch, Christian; Radmacher, Manfred
- Biophysical Journal, Vol. 78, Issue 1
Measuring the viscoelastic properties of human platelets with the atomic force microscope
journal, January 1996
- Radmacher, M.; Fritz, M.; Kacher, C. M.
- Biophysical Journal, Vol. 70, Issue 1
Micromechanical regulation in cardiac myocytes and fibroblasts: implications for tissue remodeling
journal, February 2011
- Curtis, Matthew W.; Russell, Brenda
- Pflügers Archiv - European Journal of Physiology, Vol. 462, Issue 1
From molecules to cells: imaging soft samples with the atomic force microscope
journal, September 1992
- Radmacher, M.; Tillamnn, R.; Fritz, M.
- Science, Vol. 257, Issue 5078
Atomic force microscopy - looking at mechanosensors on the cell surface
journal, September 2012
- Heinisch, J. J.; Lipke, P. N.; Beaussart, A.
- Journal of Cell Science, Vol. 125, Issue 18
Plasmon Nano-Optics: Designing Novel Nano-Tools for Biology and Medicine
book, January 2012
- Quidant, Romain
- Plasmonics
Biological Applications of Surface Plasmon Resonance Imaging
book, January 2012
- Leroy, L.; Maillart, E.; Livache, T.
- Optical Nano- and Microsystems for Bioanalytics
Optical Imaging of Individual Plasmonic Nanoparticles in Biological Samples
journal, June 2014
- Xiao, Lehui; Yeung, Edward S.
- Annual Review of Analytical Chemistry, Vol. 7, Issue 1
Surface Plasmon Resonance Imaging Sensors: A Review
journal, February 2014
- Wong, Chi Lok; Olivo, Malini
- Plasmonics, Vol. 9, Issue 4
Biomolecular plasmonics for quantitative biology and nanomedicine
journal, August 2010
- Lee, Somin Eunice; Lee, Luke P.
- Current Opinion in Biotechnology, Vol. 21, Issue 4
Quantitative Reflection Imaging of Fixed Aplysia californica Pedal Ganglion Neurons on Nanostructured Plasmonic Crystals
journal, May 2013
- Le, An-Phong; Kang, Somi; Thompson, Lucas B.
- The Journal of Physical Chemistry B, Vol. 117, Issue 42
Neuronal Transcriptome of Aplysia: Neuronal Compartments and Circuitry
journal, December 2006
- Moroz, Leonid L.; Edwards, John R.; Puthanveettil, Sathyanarayanan V.
- Cell, Vol. 127, Issue 7
Quantitative multispectral biosensing and 1D imaging using quasi-3D plasmonic crystals
journal, November 2006
- Stewart, M. E.; Mack, N. H.; Malyarchuk, V.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 46, p. 17143-17148
Seeing Molecules by Eye: Surface Plasmon Resonance Imaging at Visible Wavelengths with High Spatial Resolution and Submonolayer Sensitivity
journal, June 2008
- Yao, Jimin; Stewart, Matthew E.; Maria, Joana
- Angewandte Chemie International Edition, Vol. 47, Issue 27, p. 5013-5017
Nanostructured Plasmonic Sensors
journal, February 2008
- Stewart, Matthew E.; Anderton, Christopher R.; Thompson, Lucas B.
- Chemical Reviews, Vol. 108, Issue 2
Multispectral Thin Film Biosensing and Quantitative Imaging Using 3D Plasmonic Crystals
journal, August 2009
- Stewart, Matthew E.; Yao, Jimin; Maria, Joana
- Analytical Chemistry, Vol. 81, Issue 15
Functional Nanostructured Plasmonic Materials
journal, January 2010
- Yao, Jimin; Le, An-Phong; Gray, Stephen K.
- Advanced Materials, Vol. 22, Issue 10, p. 1102-1110
Production of Nitric Oxide within the Aplysia californica Nervous System
journal, December 2009
- Ye, Xiaoying; Xie, Fang; Romanova, Elena V.
- ACS Chemical Neuroscience, Vol. 1, Issue 3
A Novel Pyridoxal 5′-Phosphate-dependent Amino Acid Racemase in the Aplysia californica Central Nervous System
journal, April 2011
- Wang, Liping; Ota, Nobutoshi; Romanova, Elena V.
- Journal of Biological Chemistry, Vol. 286, Issue 15
1. Ultrathin Multilayer Polyelectrolyte Films on Gold: Construction and Thickness Determination
journal, June 1997
- Caruso, Frank; Niikura, Kenichi; Furlong, D. Neil
- Langmuir, Vol. 13, Issue 13
pH dependent growth of poly(L-lysine)/poly(L-glutamic) acid multilayer films and their cell adhesion properties
journal, October 2004
- Richert, Ludovic; Arntz, Youri; Schaaf, Pierre
- Surface Science, Vol. 570, Issue 1-2
Measurements of light scattering by particles in water
book, January 2007
- Jonasz, Miroslaw; Fournier, Georges R.
- Light Scattering by Particles in Water
The Density and Refractive Index of Adsorbing Protein Layers
journal, July 2004
- Vörös, Janos
- Biophysical Journal, Vol. 87, Issue 1
The Determination of Thickness and Surface Mass Density of Mesothick Immunoprecipitate Layers by Null Ellipsometry and Protein 125Iodine Labeling
journal, May 2002
- Benesch, Johan; Askendal, Agneta; Tengvall, Pentti
- Journal of Colloid and Interface Science, Vol. 249, Issue 1
Influences of trypsin and collagenase on acetylcholine responses of physically isolated single neurons ofAplysia californica
journal, June 1990
- Oyama, Y.; Hori, N.; Allen, C. N.
- Cellular and Molecular Neurobiology, Vol. 10, Issue 2
Cell detachment activates the Hippo pathway via cytoskeleton reorganization to induce anoikis
journal, January 2012
- Zhao, B.; Li, L.; Wang, L.
- Genes & Development, Vol. 26, Issue 1
Laser-Induced Cell Detachment and Patterning with Photodegradable Polymer Substrates
journal, March 2011
- Pasparakis, George; Manouras, Theodore; Selimis, Alexandros
- Angewandte Chemie International Edition, Vol. 50, Issue 18
Works referencing / citing this record:
A Nanopipette Platform for Delivering Nanoliter Volumes of Acetylcholine
journal, January 2018
- McAllister, A. B.; Menchaca, C. X.; Freeman, J.
- Journal of The Electrochemical Society, Vol. 165, Issue 12
A high spatiotemporal study of somatic exocytosis with scanning electrochemical microscopy and nanoITIES electrodes
journal, January 2018
- Welle, Theresa M.; Alanis, Kristen; Colombo, Michelle L.
- Chemical Science, Vol. 9, Issue 22