The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Rautengarten, Carsten; Ebert, Berit; Liu, Lifeng; Stonebloom, Solomon; Smith-Moritz, Andreia M.; Pauly, Markus; Orellana, Ariel; Scheller, Henrik Vibe; Heazlewood, Joshua L.

doi:10.1038/ncomms12119

Title: The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Journal Article · Wed Jul 06 04:00:00 UTC 2016 · Nature Communications

DOI: https://doi.org/10.1038/ncomms12119 · OSTI ID:1379504

Rautengarten, Carsten ^[1]; Ebert, Berit ^[2]; Liu, Lifeng ^[3]; Stonebloom, Solomon ^[4]; Smith-Moritz, Andreia M. ^[4]; Pauly, Markus ^[3]; Orellana, Ariel ^[5]; Scheller, Henrik Vibe ^[6]; Heazlewood, Joshua L. ^[1]

Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.; Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls
Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls; Univ. of Copenhagen (Denmark). Dept. of Plant and Environmental Sciences
Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
Andres Bello National Univ., Santiago (Chile). Biotechnology Center; Center for Genome Regulation, Santiago (Chile)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1379504

Journal Information:: Nature Communications, Journal Name: Nature Communications Vol. 7; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Characterization of AtNST-KT1, a novel UDP-galactose transporter from Arabidopsis thaliana Rollwitz, Inga; Santaella, Marcella; Hille, Diana FEBS Letters, Vol. 580, Issue 17 https://doi.org/10.1016/j.febslet.2006.06.082	journal	July 2006
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Suppression of Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan-II Sechet, Julien; Htwe, Soe; Urbanowicz, Breeanna The Plant Journal, Vol. 96, Issue 5 https://doi.org/10.1111/tpj.14088	journal	October 2018
Xylose donor transport is critical for fungal virulence Li, Lucy X.; Rautengarten, Carsten; Heazlewood, Joshua L. PLOS Pathogens, Vol. 14, Issue 1 https://doi.org/10.1371/journal.ppat.1006765	journal	January 2018
Elucidating Drought Stress Tolerance in European Oaks Through Cross-Species Transcriptomics Madritsch, Silvia; Wischnitzki, Elisabeth; Kotrade, Peter G3 Genes\|Genomes\|Genetics, Vol. 9, Issue 10 https://doi.org/10.1534/g3.119.400456	journal	October 2019
Characterization of a GDP-Fucose Transporter and a Fucosyltransferase Involved in the Fucosylation of Glycoproteins in the Diatom Phaeodactylum tricornutum Zhang, Peiqing; Burel, Carole; Plasson, Carole Frontiers in Plant Science, Vol. 10 https://doi.org/10.3389/fpls.2019.00610	journal	May 2019
Additional file 1 of OsUGE2 Regulates Plant Growth through Affecting ROS Homeostasis and Iron Level in Rice Yang, Shuaiqi; Chen, Nana; Qi, Jiaxuan figshare https://doi.org/10.6084/m9.figshare.24987750.v1	image	January 2024
A Golgi UDP-GlcNAc transporter delivers substrates for N-linked glycans and sphingolipids Ebert, Berit; Rautengarten, Carsten; McFarlane, Heather E. Nature Plants, Vol. 4, Issue 10 https://doi.org/10.1038/s41477-018-0235-5	journal	September 2018
Optimization of nucleotide sugar supply for polysaccharide formation via thermodynamic buffering Kleczkowski, Leszek A.; Igamberdiev, Abir U. Biochemical Journal, Vol. 477, Issue 2 https://doi.org/10.1042/bcj20190807	journal	January 2020
Conserved Glu-47 and Lys-50 residues are critical for UDP- N -acetylglucosamine/UMP antiport activity of the mouse Golgi-associated transporter Slc35a3 Toscanini, M. Agustina; Favarolo, M. Belén; Gonzalez Flecha, F. Luis Journal of Biological Chemistry, Vol. 294, Issue 26 https://doi.org/10.1074/jbc.ra119.008827	journal	May 2019
Xyloglucans fucosylation defects do not alter plant boundary domain definition Gonçalves, Beatriz; Sechet, Julien; Arnaud, Nicolas Plant Signaling & Behavior, Vol. 13, Issue 2 https://doi.org/10.1080/15592324.2018.1430545	journal	February 2018
A hypomorphic allele of SLC35D1 results in Schneckenbecken-like dysplasia Rautengarten, Carsten; Quarrell, Oliver W.; Stals, Karen Human Molecular Genetics, Vol. 28, Issue 21 https://doi.org/10.1093/hmg/ddz200	journal	August 2019
Regulation of carbon metabolism in two maize sister lines contrasted for chilling tolerance Garzon, Catalina Duran; Lequart, Michelle; Rautengarten, Carsten Journal of Experimental Botany, Vol. 71, Issue 1 https://doi.org/10.1093/jxb/erz421	journal	September 2019
The Three Members of the Arabidopsis Glycosyltransferase Family 92 are Functional β-1,4-Galactan Synthases Ebert, Berit; Birdseye, Devon; Liwanag, April J. M. Plant and Cell Physiology, Vol. 59, Issue 12 https://doi.org/10.1093/pcp/pcy180	journal	September 2018
Growth of the Fucus embryo: insights into wall-mediated cell expansion through mechanics and transcriptomics Linardić, Marina; Cokus, Shawn J.; Pellegrini, Matteo BioRxiv https://doi.org/10.1101/2020.01.29.925107	posted_content	January 2020
Golgi-localized cation/proton exchangers regulate ionic homeostasis and skotomorphogenesis in Arabidopsis: Golgi-Localized KEAs Regulate Ionic Homeostasis Wang, Yuan; Tang, Ren-Jie; Yang, Xiyan Plant, Cell & Environment, Vol. 42, Issue 2 https://doi.org/10.1111/pce.13452	journal	November 2018
Sucrose transport and carbon fluxes during wood formation Mahboubi, Amir; Niittylä, Totte Physiologia Plantarum, Vol. 164, Issue 1 https://doi.org/10.1111/ppl.12729	journal	July 2018
Suppression of Arabidopsis GGLT1 affects growth by reducing the L-galactose content and borate cross-linking of rhamnogalacturonan-II Sechet, Julien; Htwe, Soe; Urbanowicz, Breeanna The Plant Journal, Vol. 96, Issue 5 https://doi.org/10.1111/tpj.14088	journal	October 2018
UDP-Glucuronic Acid Transport Is Required for Virulence of Cryptococcus neoformans Li, Lucy X.; Rautengarten, Carsten; Heazlewood, Joshua L. mBio, Vol. 9, Issue 1 https://doi.org/10.1128/mbio.02319-17	journal	January 2018
Xylose donor transport is critical for fungal virulence Li, Lucy X.; Rautengarten, Carsten; Heazlewood, Joshua L. PLOS Pathogens, Vol. 14, Issue 1 https://doi.org/10.1371/journal.ppat.1006765	journal	January 2018

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