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Title: The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development

Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.
Authors:
 [1] ;  [2] ;  [3] ;  [4] ;  [4] ;  [3] ;  [5] ;  [6] ;  [1]
  1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.; Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls
  2. Univ. of Melbourne (Australia). ARC Centre of Excellence in Plant Cell Walls; Univ. of Copenhagen (Denmark). Dept. of Plant and Environmental Sciences
  3. Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
  4. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.
  5. Andres Bello National Univ., Santiago (Chile). Biotechnology Center; Center for Genome Regulation, Santiago (Chile)
  6. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Joint BioEnergy Inst.; Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology
Publication Date:
Grant/Contract Number:
AC02-05CH11231
Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 7; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Research Org:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; plant development; plant transporters
OSTI Identifier:
1379504

Rautengarten, Carsten, Ebert, Berit, Liu, Lifeng, Stonebloom, Solomon, Smith-Moritz, Andreia M., Pauly, Markus, Orellana, Ariel, Scheller, Henrik Vibe, and Heazlewood, Joshua L.. The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development. United States: N. p., Web. doi:10.1038/ncomms12119.
Rautengarten, Carsten, Ebert, Berit, Liu, Lifeng, Stonebloom, Solomon, Smith-Moritz, Andreia M., Pauly, Markus, Orellana, Ariel, Scheller, Henrik Vibe, & Heazlewood, Joshua L.. The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development. United States. doi:10.1038/ncomms12119.
Rautengarten, Carsten, Ebert, Berit, Liu, Lifeng, Stonebloom, Solomon, Smith-Moritz, Andreia M., Pauly, Markus, Orellana, Ariel, Scheller, Henrik Vibe, and Heazlewood, Joshua L.. 2016. "The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development". United States. doi:10.1038/ncomms12119. https://www.osti.gov/servlets/purl/1379504.
@article{osti_1379504,
title = {The Arabidopsis Golgi-localized GDP-L-fucose transporter is required for plant development},
author = {Rautengarten, Carsten and Ebert, Berit and Liu, Lifeng and Stonebloom, Solomon and Smith-Moritz, Andreia M. and Pauly, Markus and Orellana, Ariel and Scheller, Henrik Vibe and Heazlewood, Joshua L.},
abstractNote = {Nucleotide sugar transport across Golgi membranes is essential for the luminal biosynthesis of glycan structures. Here we identify GDP-fucose transporter 1 (GFT1), an Arabidopsis nucleotide sugar transporter that translocates GDP-L-fucose into the Golgi lumen. Using proteo-liposome-based transport assays, we show that GFT preferentially transports GDP-L-fucose over other nucleotide sugars in vitro, while GFT1-silenced plants are almost devoid of L-fucose in cell wall-derived xyloglucan and rhamnogalacturonan II. Furthermore, these lines display reduced L-fucose content in N-glycan structures accompanied by severe developmental growth defects. We conclude that GFT1 is the major nucleotide sugar transporter for import of GDP-L-fucose into the Golgi and is required for proper plant growth and development.},
doi = {10.1038/ncomms12119},
journal = {Nature Communications},
number = ,
volume = 7,
place = {United States},
year = {2016},
month = {7}
}

Works referenced in this record:

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A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures
journal, July 1962