Whole-Brain Mapping of Neuronal Activity in the Learned Helplessness Model of Depression
Abstract
Some individuals are resilient, whereas others succumb to despair in repeated stressful situations. The neurobiological mechanisms underlying such divergent behavioral responses remain unclear. Here, we employed an automated method for mapping neuronal activity in search of signatures of stress responses in the entire mouse brain. We used serial two-photon tomography to detect expression of c-FosGFP – a marker of neuronal activation – in c-fosGFP transgenic mice subjected to the learned helplessness (LH) procedure, a widely used model of stress-induced depression-like phenotype in laboratory animals. We found that mice showing “helpless” behavior had an overall brain-wide reduction in the level of neuronal activation compared with mice showing “resilient” behavior, with the exception of a few brain areas, including the locus coeruleus, that were more activated in the helpless mice. In addition, the helpless mice showed a strong trend of having higher similarity in whole-brain activity profile among individuals, suggesting that helplessness is represented by a more stereotypic brain-wide activation pattern. This latter effect was confirmed in rats subjected to the LH procedure, using 2-deoxy-2[18F]fluoro-D-glucose positron emission tomography to assess neural activity. In conclusion, our findings reveal distinct brain activity markings that correlate with adaptive and maladaptive behavioral responses tostress, and providemore »
- Authors:
-
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States); Penn State Univ., Hershey, PA (United States)
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States); MRC Lab. of Molecular Biology, Cambridge (United Kingdom)
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States); Brookhaven National Lab. (BNL), Upton, NY (United States); Quinnipiac Univ., Hamden, CT (United States)
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States)
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States); Brookhaven National Lab. (BNL), Upton, NY (United States); Mount Sinai School of Medicine, New York, NY (United States)
- Publication Date:
- Research Org.:
- Cold Spring Harbor Lab., Cold Spring Harbor, NY (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1378931
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Neural Circuits
- Additional Journal Information:
- Journal Volume: 10; Journal ID: ISSN 1662-5110
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; C-fos expression; serial two-photon tomography; Positron-emission tomography; learned helplessness; depression
Citation Formats
Kim, Yongsoo, Perova, Zinaida, Mirrione, Martine M., Pradhan, Kith, Henn, Fritz A., Shea, Stephen, Osten, Pavel, and Li, Bo. Whole-Brain Mapping of Neuronal Activity in the Learned Helplessness Model of Depression. United States: N. p., 2016.
Web. doi:10.3389/fncir.2016.00003.
Kim, Yongsoo, Perova, Zinaida, Mirrione, Martine M., Pradhan, Kith, Henn, Fritz A., Shea, Stephen, Osten, Pavel, & Li, Bo. Whole-Brain Mapping of Neuronal Activity in the Learned Helplessness Model of Depression. United States. https://doi.org/10.3389/fncir.2016.00003
Kim, Yongsoo, Perova, Zinaida, Mirrione, Martine M., Pradhan, Kith, Henn, Fritz A., Shea, Stephen, Osten, Pavel, and Li, Bo. Wed .
"Whole-Brain Mapping of Neuronal Activity in the Learned Helplessness Model of Depression". United States. https://doi.org/10.3389/fncir.2016.00003. https://www.osti.gov/servlets/purl/1378931.
@article{osti_1378931,
title = {Whole-Brain Mapping of Neuronal Activity in the Learned Helplessness Model of Depression},
author = {Kim, Yongsoo and Perova, Zinaida and Mirrione, Martine M. and Pradhan, Kith and Henn, Fritz A. and Shea, Stephen and Osten, Pavel and Li, Bo},
abstractNote = {Some individuals are resilient, whereas others succumb to despair in repeated stressful situations. The neurobiological mechanisms underlying such divergent behavioral responses remain unclear. Here, we employed an automated method for mapping neuronal activity in search of signatures of stress responses in the entire mouse brain. We used serial two-photon tomography to detect expression of c-FosGFP – a marker of neuronal activation – in c-fosGFP transgenic mice subjected to the learned helplessness (LH) procedure, a widely used model of stress-induced depression-like phenotype in laboratory animals. We found that mice showing “helpless” behavior had an overall brain-wide reduction in the level of neuronal activation compared with mice showing “resilient” behavior, with the exception of a few brain areas, including the locus coeruleus, that were more activated in the helpless mice. In addition, the helpless mice showed a strong trend of having higher similarity in whole-brain activity profile among individuals, suggesting that helplessness is represented by a more stereotypic brain-wide activation pattern. This latter effect was confirmed in rats subjected to the LH procedure, using 2-deoxy-2[18F]fluoro-D-glucose positron emission tomography to assess neural activity. In conclusion, our findings reveal distinct brain activity markings that correlate with adaptive and maladaptive behavioral responses tostress, and provide a framework for further studies investigating the contribution of specific brain regions to maladaptive stress responses.},
doi = {10.3389/fncir.2016.00003},
journal = {Frontiers in Neural Circuits},
number = ,
volume = 10,
place = {United States},
year = {Wed Feb 03 00:00:00 EST 2016},
month = {Wed Feb 03 00:00:00 EST 2016}
}
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Sniffing out the contributions of the olfactory tubercle to the sense of smell: Hedonics, sensory integration, and more?
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Expression of c-Fos-like immunoreactivity in the brain of mice with learned helplessness
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Are the Dorsal and Ventral Hippocampus Functionally Distinct Structures?
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