Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge
Abstract
Ebola virus (EBOV), a member of the Filoviridae that can cause severe hemorrhagic fever in humans and nonhuman primates, poses a significant threat to the public health. Currently, there are no licensed vaccines or therapeutics to prevent and treat EBOV infection. Several vaccines based on the EBOV glycoprotein (GP) are under development, including vectored, virus-like particles, and protein-based subunit vaccines. We previously demonstrated that a subunit vaccine containing the extracellular domain of the Ebola ebolavirus (EBOV) GP fused to the Fc fragment of human IgG1 (EBOVgp-Fc) protected mice against EBOV lethal challenge. Here, we show that the EBOVgp-Fc vaccine formulated with QS-21, alum, or polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) adjuvants induced strong humoral immune responses in guinea pigs. The vaccinated animals developed anti-GP total antibody titers of approximately 105–106 and neutralizing antibody titers of approximately 103 as assessed by a BSL-2 neutralization assay based on vesicular stomatitis virus (VSV) pseudotypes. The poly-ICLC formulated EBOVgp-Fc vaccine protected all the guinea pigs against EBOV lethal challenge performed under BSL-4 conditions whereas the same vaccine formulated with QS-21 or alum only induced partial protection. Vaccination with a mucin-deletedEBOVgp-Fc construct formulated with QS-21 adjuvant did not have a significant effect in anti-GP antibody levels andmore »
- Authors:
-
- Food and Drug Administration, Silver Spring, MD (United States)
- United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD (United States)
- United States Army Medical Research Institute of Infectious Diseases, Fort Detrick, MD (United States); Univ. of New Mexico, Albuquerque, NM (United States)
- Rockefeller Univ., New York, NY (United States)
- Publication Date:
- Research Org.:
- Food and Drug Administration, Silver Spring, MD (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1377872
- Grant/Contract Number:
- Y1-AI-0664-01; Intramural; DTRA IAA 11005IA-3333-Basic
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 9; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; vaccines; guinea pigs; antibodies; immunologic adjuvants; enzyme-linked immunoassays; alumni; vesicular stomatitis virus; immune response
Citation Formats
Konduru, Krishnamurthy, Shurtleff, Amy C., Bradfute, Steven B., Nakamura, Siham, Bavari, Sina, Kaplan, Gerardo, and Tsuji, Moriya. Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge. United States: N. p., 2016.
Web. doi:10.1371/journal.pone.0162446.
Konduru, Krishnamurthy, Shurtleff, Amy C., Bradfute, Steven B., Nakamura, Siham, Bavari, Sina, Kaplan, Gerardo, & Tsuji, Moriya. Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge. United States. https://doi.org/10.1371/journal.pone.0162446
Konduru, Krishnamurthy, Shurtleff, Amy C., Bradfute, Steven B., Nakamura, Siham, Bavari, Sina, Kaplan, Gerardo, and Tsuji, Moriya. Tue .
"Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge". United States. https://doi.org/10.1371/journal.pone.0162446. https://www.osti.gov/servlets/purl/1377872.
@article{osti_1377872,
title = {Ebolavirus Glycoprotein Fc Fusion Protein Protects Guinea Pigs against Lethal Challenge},
author = {Konduru, Krishnamurthy and Shurtleff, Amy C. and Bradfute, Steven B. and Nakamura, Siham and Bavari, Sina and Kaplan, Gerardo and Tsuji, Moriya},
abstractNote = {Ebola virus (EBOV), a member of the Filoviridae that can cause severe hemorrhagic fever in humans and nonhuman primates, poses a significant threat to the public health. Currently, there are no licensed vaccines or therapeutics to prevent and treat EBOV infection. Several vaccines based on the EBOV glycoprotein (GP) are under development, including vectored, virus-like particles, and protein-based subunit vaccines. We previously demonstrated that a subunit vaccine containing the extracellular domain of the Ebola ebolavirus (EBOV) GP fused to the Fc fragment of human IgG1 (EBOVgp-Fc) protected mice against EBOV lethal challenge. Here, we show that the EBOVgp-Fc vaccine formulated with QS-21, alum, or polyinosinic-polycytidylic acid-poly-L-lysine carboxymethylcellulose (poly-ICLC) adjuvants induced strong humoral immune responses in guinea pigs. The vaccinated animals developed anti-GP total antibody titers of approximately 105–106 and neutralizing antibody titers of approximately 103 as assessed by a BSL-2 neutralization assay based on vesicular stomatitis virus (VSV) pseudotypes. The poly-ICLC formulated EBOVgp-Fc vaccine protected all the guinea pigs against EBOV lethal challenge performed under BSL-4 conditions whereas the same vaccine formulated with QS-21 or alum only induced partial protection. Vaccination with a mucin-deletedEBOVgp-Fc construct formulated with QS-21 adjuvant did not have a significant effect in anti-GP antibody levels and protection against EBOV lethal challenge compared to the full-lengthGP construct. The bulk of the humoral response induced by the EBOVgp-Fc vaccine was directed against epitopes outside the EBOV mucin region. Our findings indicate that different adjuvants can eliciting varying levels of protection against lethal EBOV challenge in guinea pigs vaccinated with EBOVgp-Fc,and suggest that levels of total anti-GP antibodies elicit by protein-based GP subunit vaccines do not correlate with protection. In conclusion, our data further support the development of Fc fusions of GP as a candidate vaccine for human use.},
doi = {10.1371/journal.pone.0162446},
journal = {PLoS ONE},
number = 9,
volume = 11,
place = {United States},
year = {Tue Sep 13 00:00:00 EDT 2016},
month = {Tue Sep 13 00:00:00 EDT 2016}
}
Web of Science
Works referenced in this record:
Pathogenesis of Experimental Ebola Virus Infection in Guinea Pigs
journal, February 1999
- Connolly, Brett M.; Steele, Keith E.; Davis, Kelly J.
- The Journal of Infectious Diseases, Vol. 179, Issue s1
Nonhuman primates as models for the discovery and development of ebolavirus therapeutics
journal, January 2011
- Shurtleff, Amy C.; Warren, Travis K.; Bavari, Sina
- Expert Opinion on Drug Discovery, Vol. 6, Issue 3
Fc‐fusion proteins: new developments and future perspectives
journal, July 2012
- Czajkowsky, Daniel M.; Hu, Jun; Shao, Zhifeng
- EMBO Molecular Medicine, Vol. 4, Issue 10
Safety and immunogenicity of a chimpanzee adenovirus-vectored Ebola vaccine in healthy adults: a randomised, double-blind, placebo-controlled, dose-finding, phase 1/2a study
journal, March 2016
- De Santis, Olga; Audran, Régine; Pothin, Emilie
- The Lancet Infectious Diseases, Vol. 16, Issue 3
Marburg virus-like particles protect guinea pigs from lethal Marburg virus infection
journal, September 2004
- Warfield, Kelly L.; Swenson, Dana L.; Negley, Diane L.
- Vaccine, Vol. 22, Issue 25-26
Recombinant vesicular stomatitis viruses from DNA.
journal, May 1995
- Lawson, N. D.; Stillman, E. A.; Whitt, M. A.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 10
A system for functional analysis of Ebola virus glycoprotein
journal, December 1997
- Takada, A.; Robison, C.; Goto, H.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 26
Ebola Virus‐Like Particle–Based Vaccine Protects Nonhuman Primates against Lethal Ebola Virus Challenge
journal, November 2007
- Warfield, Kelly L.; Swenson, Dana L.; Olinger, Gene G.
- The Journal of Infectious Diseases, Vol. 196, Issue s2
Antibodies are necessary for rVSV/ZEBOV-GP-mediated protection against lethal Ebola virus challenge in nonhuman primates
journal, January 2013
- Marzi, A.; Engelmann, F.; Feldmann, F.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 5
Fc-receptors and immunity to malaria: from models to vaccines
journal, September 2009
- Pleass, R. J.
- Parasite Immunology, Vol. 31, Issue 9
Immune Parameters Correlate with Protection Against Ebola Virus Infection in Rodents and Nonhuman Primates
journal, October 2012
- Wong, G.; Richardson, J. S.; Pillet, S.
- Science Translational Medicine, Vol. 4, Issue 158
Cross-reactive HIV-1-neutralizing activity of serum IgG from a rabbit immunized with gp41 fused to IgG1 Fc: Possible role of the prolonged half-life of the immunogen
journal, February 2009
- Zhang, Mei-Yun; Wang, Yanping; Mankowski, Marie K.
- Vaccine, Vol. 27, Issue 6
Filovirius vaccines
journal, June 2011
- Bradfute, Steven B.; Dye, Jr., John M.; Bavari, Sina
- Human Vaccines, Vol. 7, Issue 6
Correlates of protective immunity for Ebola vaccines: implications for regulatory approval by the animal rule
journal, May 2009
- Sullivan, Nancy J.; Martin, Julie E.; Graham, Barney S.
- Nature Reviews Microbiology, Vol. 7, Issue 5
Perspectives on West Africa Ebola Virus Disease Outbreak, 2013–2016
journal, June 2016
- Spengler, Jessica R.; Ervin, Elizabeth D.; Towner, Jonathan S.
- Emerging Infectious Diseases, Vol. 22, Issue 6
(How) do aluminium adjuvants work?
journal, January 2006
- Brewer, J.
- Immunology Letters, Vol. 102, Issue 1
Immunogenicity of the outer domain of a HIV-1 clade C gp120
journal, January 2007
- Chen, Hongying; Xu, Xiaodong; Jones, Ian M.
- Retrovirology, Vol. 4, Issue 1
Polymorphism of Filovirus Glycoproteins
book, January 2005
- Volchkov, Viktor E.; Volchkova, Valentina A.; Dolnik, Olga
- Advances in Virus Research
Ebola virus-like particles protect from lethal Ebola virus infection
journal, December 2003
- Warfield, K. L.; Bosio, C. M.; Welcher, B. C.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 26
Biosynthesis and role of filoviral glycoproteins
journal, December 2001
- Feldmann, Heinz; Volchkov, Viktor E.; Volchkova, Valentina A.
- Journal of General Virology, Vol. 82, Issue 12
Natural and synthetic saponin adjuvant QS-21 for vaccines against cancer
journal, April 2011
- Ragupathi, Govind; Gardner, Jeffrey R.; Livingston, Philip O.
- Expert Review of Vaccines, Vol. 10, Issue 4
Live attenuated recombinant vaccine protects nonhuman primates against Ebola and Marburg viruses
journal, June 2005
- Jones, Steven M.; Feldmann, Heinz; Ströher, Ute
- Nature Medicine, Vol. 11, Issue 7
Ebola Virus Disease 2013-2014 Outbreak in West Africa: An Analysis of the Epidemic Spread and Response
journal, January 2015
- Cenciarelli, Orlando; Pietropaoli, Stefano; Malizia, Andrea
- International Journal of Microbiology, Vol. 2015
Postexposure antibody prophylaxis protects nonhuman primates from filovirus disease
journal, March 2012
- Dye, J. M.; Herbert, A. S.; Kuehne, A. I.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 13
Emergence of Zaire Ebola Virus Disease in Guinea
journal, October 2014
- Baize, Sylvain; Pannetier, Delphine; Oestereich, Lisa
- New England Journal of Medicine, Vol. 371, Issue 15
Increased potency of Fc-receptor-targeted antigens
journal, November 1997
- Guyre, P. M.; Graziano, Robert F.; Goldstein, Joel
- Cancer Immunology, Immunotherapy, Vol. 45, Issue 3-4
Newly Discovered Ebola Virus Associated with Hemorrhagic Fever Outbreak in Uganda
journal, November 2008
- Towner, Jonathan S.; Sealy, Tara K.; Khristova, Marina L.
- PLoS Pathogens, Vol. 4, Issue 11
Current perspectives on the phylogeny of Filoviridae
journal, October 2011
- Barrette, Roger W.; Xu, Lizhe; Rowland, Jessica M.
- Infection, Genetics and Evolution, Vol. 11, Issue 7
Synthetic Double-Stranded RNAs Are Adjuvants for the Induction of T Helper 1 and Humoral Immune Responses to Human Papillomavirus in Rhesus Macaques
journal, April 2009
- Stahl-Hennig, Christiane; Eisenblätter, Martin; Jasny, Edith
- PLoS Pathogens, Vol. 5, Issue 4
Replication-Deficient Ebolavirus as a Vaccine Candidate
journal, February 2009
- Halfmann, Peter; Ebihara, Hideki; Marzi, Andrea
- Journal of Virology, Vol. 83, Issue 8
Correlates of Immunity to Filovirus Infection
journal, June 2011
- Bradfute, Steven; Bavari, Sina
- Viruses, Vol. 3, Issue 7
Development of a preventive vaccine for Ebola virus infection in primates
journal, November 2000
- Sullivan, Nancy J.; Sanchez, Anthony; Rollin, Pierre E.
- Nature, Vol. 408, Issue 6812
Respiratory tract immunization of non-human primates with a Newcastle disease virus-vectored vaccine candidate against Ebola virus elicits a neutralizing antibody response
journal, December 2010
- DiNapoli, Joshua M.; Yang, Lijuan; Samal, Siba K.
- Vaccine, Vol. 29, Issue 1
Properties of Replication-Competent Vesicular Stomatitis Virus Vectors Expressing Glycoproteins of Filoviruses and Arenaviruses
journal, May 2004
- Garbutt, Michael; Liebscher, Ryan; Wahl-Jensen, Victoria
- Journal of Virology, Vol. 78, Issue 10
Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates
journal, August 2003
- Sullivan, Nancy J.; Geisbert, Thomas W.; Geisbert, Joan B.
- Nature, Vol. 424, Issue 6949
Virus nomenclature below the species level: a standardized nomenclature for laboratory animal-adapted strains and variants of viruses assigned to the family Filoviridae
journal, January 2013
- Kuhn, Jens H.; Bao, Yiming; Bavari, Sina
- Archives of Virology, Vol. 158, Issue 6
Ebola virus glycoprotein Fc fusion protein confers protection against lethal challenge in vaccinated mice
journal, April 2011
- Konduru, Krishnamurthy; Bradfute, Steven B.; Jacques, Jerome
- Vaccine, Vol. 29, Issue 16
Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations
journal, October 2010
- Kuhn, Jens H.; Becker, Stephan; Ebihara, Hideki
- Archives of Virology, Vol. 155, Issue 12
Identification of the Ebola virus glycoprotein as the main viral determinant of vascular cell cytotoxicity and injury
journal, August 2000
- Nabel, Gary J.; Yang, Zhi-yong; Duckers, Henricus J.
- Nature Medicine, Vol. 6, Issue 8, p. 886-889
Marburg Virus Vaccines Based upon Alphavirus Replicons Protect Guinea Pigs and Nonhuman Primates
journal, November 1998
- Hevey, Michael; Negley, Diane; Pushko, Peter
- Virology, Vol. 251, Issue 1
Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients
journal, April 1999
- Baize, Sylvain; Leroy, Eric M.; Georges-Courbot, M. -C.
- Nature Medicine, Vol. 5, Issue 4
Immune Protection of Nonhuman Primates against Ebola Virus with Single Low-Dose Adenovirus Vectors Encoding Modified GPs
journal, May 2006
- Sullivan, Nancy J.; Geisbert, Thomas W.; Geisbert, Joan B.
- PLoS Medicine, Vol. 3, Issue 6
Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor
journal, July 2008
- Lee, Jeffrey E.; Fusco, Marnie L.; Hessell, Ann J.
- Nature, Vol. 454, Issue 7201
Synthetic double-stranded RNA induces innate immune responses similar to a live viral vaccine in humans
journal, November 2011
- Caskey, Marina; Lefebvre, François; Filali-Mouhim, Abdelali
- Journal of Experimental Medicine, Vol. 208, Issue 12
Mucosal Immunization of Cynomolgus Macaques with the VSVΔG/ZEBOVGP Vaccine Stimulates Strong Ebola GP-Specific Immune Responses
journal, May 2009
- Qiu, Xiangguo; Fernando, Lisa; Alimonti, Judie B.
- PLoS ONE, Vol. 4, Issue 5
Effective Post-Exposure Treatment of Ebola Infection
journal, January 2007
- Feldmann, Heinz; Jones, Steven M.; Daddario-DiCaprio, Kathleen M.
- PLoS Pathogens, Vol. 3, Issue 1
Ebola and Marburg Hemorrhagic Fever
journal, March 2010
- Hartman, Amy L.; Towner, Jonathan S.; Nichol, Stuart T.
- Clinics in Laboratory Medicine, Vol. 30, Issue 1
Ebola virus vaccines: an overview of current approaches
journal, February 2014
- Marzi, Andrea; Feldmann, Heinz
- Expert Review of Vaccines, Vol. 13, Issue 4
Ebola virus: from discovery to vaccine
journal, August 2003
- Feldmann, Heinz; Jones, Steven; Klenk, Hans-Dieter
- Nature Reviews Immunology, Vol. 3, Issue 8
Action of Saponin on Biological Cell Membranes
journal, December 1962
- Bangham, A. D.; Horne, R. W.
- Nature, Vol. 196, Issue 4858
Generation of Bovine Respiratory Syncytial Virus (BRSV) from cDNA: BRSV NS2 Is Not Essential for Virus Replication in Tissue Culture, and the Human RSV Leader Region Acts as a Functional BRSV Genome Promoter
journal, January 1999
- Buchholz, Ursula J.; Finke, Stefan; Conzelmann, Karl-Klaus
- Journal of Virology, Vol. 73, Issue 1
The minimal conserved transcription stop-start signal promotes stable expression of a foreign gene in vesicular stomatitis virus.
journal, January 1996
- Schnell, M. J.; Buonocore, L.; Whitt, M. A.
- Journal of virology, Vol. 70, Issue 4
Fc‐fusion proteins: new developments and future perspectives
journal, July 2012
- Czajkowsky, Daniel M.; Hu, Jun; Shao, Zhifeng
- EMBO Molecular Medicine, Vol. 4, Issue 10
Increased potency of Fc-receptor-targeted antigens
journal, November 1997
- Guyre, P. M.; Graziano, Robert F.; Goldstein, Joel
- Cancer Immunology, Immunotherapy, Vol. 45, Issue 3-4
Proposal for a revised taxonomy of the family Filoviridae: classification, names of taxa and viruses, and virus abbreviations
journal, October 2010
- Kuhn, Jens H.; Becker, Stephan; Ebihara, Hideki
- Archives of Virology, Vol. 155, Issue 12
Virus nomenclature below the species level: a standardized nomenclature for laboratory animal-adapted strains and variants of viruses assigned to the family Filoviridae
journal, January 2013
- Kuhn, Jens H.; Bao, Yiming; Bavari, Sina
- Archives of Virology, Vol. 158, Issue 6
(How) do aluminium adjuvants work?
journal, January 2006
- Brewer, J.
- Immunology Letters, Vol. 102, Issue 1
Current perspectives on the phylogeny of Filoviridae
journal, October 2011
- Barrette, Roger W.; Xu, Lizhe; Rowland, Jessica M.
- Infection, Genetics and Evolution, Vol. 11, Issue 7
Marburg virus-like particles protect guinea pigs from lethal Marburg virus infection
journal, September 2004
- Warfield, Kelly L.; Swenson, Dana L.; Negley, Diane L.
- Vaccine, Vol. 22, Issue 25-26
Cross-reactive HIV-1-neutralizing activity of serum IgG from a rabbit immunized with gp41 fused to IgG1 Fc: Possible role of the prolonged half-life of the immunogen
journal, February 2009
- Zhang, Mei-Yun; Wang, Yanping; Mankowski, Marie K.
- Vaccine, Vol. 27, Issue 6
Respiratory tract immunization of non-human primates with a Newcastle disease virus-vectored vaccine candidate against Ebola virus elicits a neutralizing antibody response
journal, December 2010
- DiNapoli, Joshua M.; Yang, Lijuan; Samal, Siba K.
- Vaccine, Vol. 29, Issue 1
Ebola virus glycoprotein Fc fusion protein confers protection against lethal challenge in vaccinated mice
journal, April 2011
- Konduru, Krishnamurthy; Bradfute, Steven B.; Jacques, Jerome
- Vaccine, Vol. 29, Issue 16
Aluminum compounds as vaccine adjuvants
journal, July 1998
- Gupta, R.
- Advanced Drug Delivery Reviews, Vol. 32, Issue 3
Ebola vaccines: we have options
journal, March 2016
- Falzarano, Darryl
- The Lancet Infectious Diseases, Vol. 16, Issue 3
Development of a preventive vaccine for Ebola virus infection in primates
journal, November 2000
- Sullivan, Nancy J.; Sanchez, Anthony; Rollin, Pierre E.
- Nature, Vol. 408, Issue 6812
Defective humoral responses and extensive intravascular apoptosis are associated with fatal outcome in Ebola virus-infected patients
journal, April 1999
- Baize, Sylvain; Leroy, Eric M.; Georges-Courbot, M. -C.
- Nature Medicine, Vol. 5, Issue 4
Accelerated vaccination for Ebola virus haemorrhagic fever in non-human primates
journal, August 2003
- Sullivan, Nancy J.; Geisbert, Thomas W.; Geisbert, Joan B.
- Nature, Vol. 424, Issue 6949
Structure of the Ebola virus glycoprotein bound to an antibody from a human survivor
journal, July 2008
- Lee, Jeffrey E.; Fusco, Marnie L.; Hessell, Ann J.
- Nature, Vol. 454, Issue 7201
Ebola virus: from discovery to vaccine
journal, August 2003
- Feldmann, Heinz; Jones, Steven; Klenk, Hans-Dieter
- Nature Reviews Immunology, Vol. 3, Issue 8
Correlates of protective immunity for Ebola vaccines: implications for regulatory approval by the animal rule
journal, May 2009
- Sullivan, Nancy J.; Martin, Julie E.; Graham, Barney S.
- Nature Reviews Microbiology, Vol. 7, Issue 5
Phase 1 Trials of rVSV Ebola Vaccine in Africa and Europe
journal, April 2016
- Agnandji, Selidji T.; Huttner, Angela; Zinser, Madeleine E.
- New England Journal of Medicine, Vol. 374, Issue 17
Postexposure antibody prophylaxis protects nonhuman primates from filovirus disease
journal, March 2012
- Dye, J. M.; Herbert, A. S.; Kuehne, A. I.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 13
Antibodies are necessary for rVSV/ZEBOV-GP-mediated protection against lethal Ebola virus challenge in nonhuman primates
journal, January 2013
- Marzi, A.; Engelmann, F.; Feldmann, F.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 5
Delayed treatment of Ebola virus infection with plant-derived monoclonal antibodies provides protection in rhesus macaques
journal, October 2012
- Olinger, G. G.; Pettitt, J.; Kim, D.
- Proceedings of the National Academy of Sciences, Vol. 109, Issue 44
Recombinant vesicular stomatitis viruses from DNA.
journal, May 1995
- Lawson, N. D.; Stillman, E. A.; Whitt, M. A.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 10
A system for functional analysis of Ebola virus glycoprotein
journal, December 1997
- Takada, A.; Robison, C.; Goto, H.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 26
Synthetic double-stranded RNA induces innate immune responses similar to a live viral vaccine in humans
journal, November 2011
- Caskey, Marina; Lefebvre, François; Filali-Mouhim, Abdelali
- Journal of Experimental Medicine, Vol. 208, Issue 12
Ebola Virus‐Like Particle–Based Vaccine Protects Nonhuman Primates against Lethal Ebola Virus Challenge
journal, November 2007
- Warfield, Kelly L.; Swenson, Dana L.; Olinger, Gene G.
- The Journal of Infectious Diseases, Vol. 196, Issue s2
Fc-receptors and immunity to malaria: from models to vaccines
journal, September 2009
- Pleass, R. J.
- Parasite Immunology, Vol. 31, Issue 9
Successful Treatment of Ebola Virus-Infected Cynomolgus Macaques with Monoclonal Antibodies
journal, June 2012
- Qiu, X.; Audet, J.; Wong, G.
- Science Translational Medicine, Vol. 4, Issue 138
Immune Parameters Correlate with Protection Against Ebola Virus Infection in Rodents and Nonhuman Primates
journal, October 2012
- Wong, G.; Richardson, J. S.; Pillet, S.
- Science Translational Medicine, Vol. 4, Issue 158
A New Ebola Virus Nonstructural Glycoprotein Expressed through RNA Editing
journal, March 2011
- Mehedi, M.; Falzarano, D.; Seebach, J.
- Journal of Virology, Vol. 85, Issue 11
Ebola Virus Can Be Effectively Neutralized by Antibody Produced in Natural Human Infection
journal, July 1999
- Maruyama, Toshiaki; Rodriguez, Luis L.; Jahrling, Peter B.
- Journal of Virology, Vol. 73, Issue 7
Analysis of Human Peripheral Blood Samples from Fatal and Nonfatal Cases of Ebola (Sudan) Hemorrhagic Fever: Cellular Responses, Virus Load, and Nitric Oxide Levels
journal, September 2004
- Sanchez, A.; Lukwiya, M.; Bausch, D.
- Journal of Virology, Vol. 78, Issue 19
Transmission of Ebola Viruses: What We Know and What We Do Not Know
journal, February 2015
- Osterholm, Michael T.; Moore, Kristine A.; Kelley, Nicholas S.
- mBio, Vol. 6, Issue 2
Immunogenicity of the outer domain of a HIV-1 clade C gp120
journal, January 2007
- Chen, Hongying; Xu, Xiaodong; Jones, Ian M.
- Retrovirology, Vol. 4, Issue 1
Immune Protection of Nonhuman Primates against Ebola Virus with Single Low-Dose Adenovirus Vectors Encoding Modified GPs
journal, May 2006
- Sullivan, Nancy J.; Geisbert, Thomas W.; Geisbert, Joan B.
- PLoS Medicine, Vol. 3, Issue 6
Mucosal Immunization of Cynomolgus Macaques with the VSVΔG/ZEBOVGP Vaccine Stimulates Strong Ebola GP-Specific Immune Responses
journal, May 2009
- Qiu, Xiangguo; Fernando, Lisa; Alimonti, Judie B.
- PLoS ONE, Vol. 4, Issue 5
Effective Post-Exposure Treatment of Ebola Infection
journal, January 2007
- Feldmann, Heinz; Jones, Steven M.; Daddario-DiCaprio, Kathleen M.
- PLoS Pathogens, Vol. 3, Issue 1
Synthetic Double-Stranded RNAs Are Adjuvants for the Induction of T Helper 1 and Humoral Immune Responses to Human Papillomavirus in Rhesus Macaques
journal, April 2009
- Stahl-Hennig, Christiane; Eisenblätter, Martin; Jasny, Edith
- PLoS Pathogens, Vol. 5, Issue 4
Nonhuman primates as models for the discovery and development of ebolavirus therapeutics
journal, January 2011
- Shurtleff, Amy C.; Warren, Travis K.; Bavari, Sina
- Expert Opinion on Drug Discovery, Vol. 6, Issue 3
Ebola virus vaccines: an overview of current approaches
journal, February 2014
- Marzi, Andrea; Feldmann, Heinz
- Expert Review of Vaccines, Vol. 13, Issue 4
Natural and synthetic saponin adjuvant QS-21 for vaccines against cancer
journal, April 2011
- Ragupathi, Govind; Gardner, Jeffrey R.; Livingston, Philip O.
- Expert Review of Vaccines, Vol. 10, Issue 4
Perspectives on West Africa Ebola Virus Disease Outbreak, 2013–2016
journal, June 2016
- Spengler, Jessica R.; Ervin, Elizabeth D.; Towner, Jonathan S.
- Emerging Infectious Diseases, Vol. 22, Issue 6
Correlates of Immunity to Filovirus Infection
journal, June 2011
- Bradfute, Steven; Bavari, Sina
- Viruses, Vol. 3, Issue 7
Dead or alive: animal sampling during Ebola hemorrhagic fever outbreaks in humans
journal, January 2012
- Olson, Sarah H.; Reed, Patricia; Cameron, Kenneth N.
- Emerging Health Threats Journal, Vol. 5, Issue 1
Newly discovered Ebola virus associated with hemorrhagic fever outbreak in Uganda
text, January 2008
- Towner, Jonathan S.; Sealy, Tara K.; Khristova, Marina L.
- Columbia University
Works referencing / citing this record:
Ebola and Marburg virus vaccines
journal, April 2017
- Reynolds, Pierce; Marzi, Andrea
- Virus Genes, Vol. 53, Issue 4
Vaccines against Ebola virus and Marburg virus: recent advances and promising candidates
journal, May 2019
- Suschak, John J.; Schmaljohn, Connie S.
- Human Vaccines & Immunotherapeutics, Vol. 15, Issue 10
Advances in Designing and Developing Vaccines, Drugs, and Therapies to Counter Ebola Virus
journal, August 2018
- Dhama, Kuldeep; Karthik, Kumaragurubaran; Khandia, Rekha
- Frontiers in Immunology, Vol. 9
Saponins from Quillaja saponaria and Quillaja brasiliensis: Particular Chemical Characteristics and Biological Activities
journal, January 2019
- Fleck, Juliane Deise; Betti, Andresa Heemann; Da Silva, Francini Pereira
- Molecules, Vol. 24, Issue 1
A Novel Bacterium-Like Particle-Based Vaccine Displaying the SUDV Glycoprotein Induces Potent Humoral and Cellular Immune Responses in Mice
journal, December 2019
- Xu, Shengnan; Jiao, Cuicui; Jin, Hongli
- Viruses, Vol. 11, Issue 12
Advances in Designing and Developing Vaccines, Drugs, and Therapies to Counter Ebola Virus
journal, August 2018
- Dhama, Kuldeep; Karthik, Kumaragurubaran; Khandia, Rekha
- Frontiers in Immunology, Vol. 9