Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius

Lee, Brady D.; Apel, William A.; DeVeaux, Linda C.; Sheridan, Peter P.

doi:10.1007/s10295-017-1968-2

Title: Concurrent metabolism of pentose and hexose sugars by the polyextremophile Alicyclobacillus acidocaldarius

Journal Article · Sun Oct 01 04:00:00 UTC 2017 · Journal of Industrial Microbiology and Biotechnology

DOI: https://doi.org/10.1007/s10295-017-1968-2 · OSTI ID:1773516

Lee, Brady D. ^[1]; Apel, William A. ^[2]; DeVeaux, Linda C. ^[3]; Sheridan, Peter P. ^[4]

grid.417824.c 0000 0001 0020 7392 Idaho National Laboratory Biological Systems Department Idaho Falls ID USA, 0000 0001 2169 6535 grid.257296.d Department of Biological Sciences Idaho State University Pocatello ID USA, grid.451303.0 0000 0001 2218 3491 Pacific Northwest National Laboratory Energy and Environment Directorate Richland WA USA
grid.417824.c 0000 0001 0020 7392 Idaho National Laboratory Biological Systems Department Idaho Falls ID USA, Aspenglow Associates, LLC P. O. Box 12692 83002 Jackson WY USA
0000 0001 0704 1727 grid.263790.9 Department of Chemistry and Applied Biological Sciences South Dakota School of Mines and Technology Rapid City SD USA
0000 0001 2169 6535 grid.257296.d Department of Biological Sciences Idaho State University Pocatello ID USA

Abstract Alicyclobacillus acidocaldarius is a thermoacidophilic bacterium capable of growth on sugars from plant biomass. Carbon catabolite repression (CCR) allows bacteria to focus cellular resources on a sugar that provides efficient growth, but also allows sequential, rather than simultaneous use when more than one sugar is present. The A. acidocaldarius genome encodes all components of CCR, but transporters encoded are multifacilitator superfamily and ATP-binding cassette-type transporters, uncommon for CCR. Therefore, global transcriptome analysis of A. acidocaldarius grown on xylose or fructose was performed in chemostats, followed by attempted induction of CCR with glucose or arabinose. Alicyclobacillus acidocaldarius grew while simultaneously metabolizing xylose and glucose, xylose and arabinose, and fructose and glucose, indicating that CCR did not control carbon metabolism. Microarrays showed down-regulation of genes during growth on one sugar compared to two, and occurred primarily in genes encoding: (1) regulators; (2) enzymes for cell wall synthesis; and (3) sugar transporters.

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Sponsoring Organization:: USDOE

OSTI ID:: 1773516

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 10 Vol. 44; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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