Vibrational spectroscopy for imaging single microbial cells in complex biological samples

Harrison, Jesse P.; Berry, David

doi:10.3389/fmicb.2017.00675

Title: Vibrational spectroscopy for imaging single microbial cells in complex biological samples

Journal Article · Thu Apr 13 04:00:00 UTC 2017 · Frontiers in Microbiology

DOI: https://doi.org/10.3389/fmicb.2017.00675 · OSTI ID:1367190

Harrison, Jesse P. ^[1]; Berry, David ^[2]

Univ. of Vienna, Vienna (Austria); University of Vienna, Austria
Univ. of Vienna, Vienna (Austria)

Here, vibrational spectroscopy is increasingly used for the rapid and non-destructive imaging of environmental and medical samples. Both Raman and Fourier-transform infrared (FT-IR) imaging have been applied to obtain detailed information on the chemical composition of biological materials, ranging from single microbial cells to tissues. Due to its compatibility with methods such as stable isotope labeling for the monitoring of cellular activities, vibrational spectroscopy also holds considerable power as a tool in microbial ecology. Chemical imaging of undisturbed biological systems (such as live cells in their native habitats) presents unique challenges due to the physical and chemical complexity of the samples, potential for spectral interference, and frequent need for real-time measurements. This Mini Review provides a critical synthesis of recent applications of Raman and FT-IR spectroscopy for characterizing complex biological samples, with a focus on developments in single-cell imaging. We also discuss how new spectroscopic methods could be used to overcome current limitations of singlecell analyses. Given the inherent complementarity of Raman and FT-IR spectroscopic methods, we discuss how combining these approaches could enable us to obtain new insights into biological activities either in situ or under conditions that simulate selected properties of the natural environment.

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Research Organization:: Univ. of North Carolina, Chapel Hill, NC (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0013887

OSTI ID:: 1367190

Journal Information:: Frontiers in Microbiology, Journal Name: Frontiers in Microbiology Vol. 8; ISSN 1664-302X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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Label-Free Imaging and Spectroscopic Analysis of Intracellular Bacterial Infections Große, Christina; Bergner, Norbert; Dellith, Jan Analytical Chemistry, Vol. 87, Issue 4 https://doi.org/10.1021/ac503316s	journal	January 2015
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Combining Raman and FT-IR Spectroscopy with Quantitative Isotopic Labeling for Differentiation of E. coli Cells at Community and Single Cell Levels Muhamadali, Howbeer; Chisanga, Malama; Subaihi, Abdu Analytical Chemistry, Vol. 87, Issue 8 https://doi.org/10.1021/acs.analchem.5b00892	journal	April 2015
Development of Visible-Wavelength MALDI Cell Mass Spectrometry for High-Efficiency Single-Cell Analysis Xiong, Caiqiao; Zhou, Xiaoyu; He, Qing Analytical Chemistry, Vol. 88, Issue 23 https://doi.org/10.1021/acs.analchem.6b03789	journal	November 2016
Nanoscale Chemical Imaging of Bacillus subtilis Spores by Combining Tip-Enhanced Raman Scattering and Advanced Statistical Tools Rusciano, Giulia; Zito, Gianluigi; Isticato, Rachele ACS Nano, Vol. 8, Issue 12 https://doi.org/10.1021/nn504595k	journal	November 2014
Micron-scale mapping of sulfur cycling across the oxycline of a cyanobacterial mat: a paired nanoSIMS and CARD-FISH approach Fike, David Andrew; Gammon, Crystal Lynn; Ziebis, Wiebke The ISME Journal, Vol. 2, Issue 7 https://doi.org/10.1038/ismej.2008.39	journal	June 2008
Rapid resonance Raman microspectroscopy to probe carbon dioxide fixation by single cells in microbial communities Li, Mengqiu; Canniffe, Daniel P.; Jackson, Philip J. The ISME Journal, Vol. 6, Issue 4 https://doi.org/10.1038/ismej.2011.150	journal	November 2011
Privatization of cooperative benefits stabilizes mutualistic cross-feeding interactions in spatially structured environments Pande, Samay; Kaftan, Filip; Lang, Stefan The ISME Journal, Vol. 10, Issue 6 https://doi.org/10.1038/ismej.2015.212	journal	December 2015
Hyperspectral infrared nanoimaging of organic samples based on Fourier transform infrared nanospectroscopy Amenabar, Iban; Poly, Simon; Goikoetxea, Monika Nature Communications, Vol. 8, Issue 1 https://doi.org/10.1038/ncomms14402	journal	February 2017
High-resolution Fourier-transform infrared chemical imaging with multiple synchrotron beams Nasse, Michael J.; Walsh, Michael J.; Mattson, Eric C. Nature Methods, Vol. 8, Issue 5 https://doi.org/10.1038/nmeth.1585	journal	March 2011
Self-organized patchiness facilitates survival in a cooperatively growing Bacillus subtilis population Ratzke, Christoph; Gore, Jeff Nature Microbiology, Vol. 1, Issue 5 https://doi.org/10.1038/nmicrobiol.2016.22	journal	March 2016
Chemically sensitive bioimaging with coherent Raman scattering Camp Jr, Charles H.; Cicerone, Marcus T. Nature Photonics, Vol. 9, Issue 5 https://doi.org/10.1038/nphoton.2015.60	journal	April 2015
Rapid discrimination of Enterococcus faecium strains using phenotypic analytical techniques AlMasoud, Najla; Xu, Yun; Ellis, David I. Analytical Methods, Vol. 8, Issue 42 https://doi.org/10.1039/C6AY02326F	journal	January 2016
Raman and CARS microspectroscopy of cells and tissues Krafft, Christoph; Dietzek, Benjamin; Popp, Jürgen The Analyst, Vol. 134, Issue 6 https://doi.org/10.1039/b822354h	journal	January 2009
Applications of modern micro-Raman spectroscopy for cell analyses Mariani, Melissa M.; Day, Philip J. R.; Deckert, Volker Integrative Biology, Vol. 2, Issue 2-3 https://doi.org/10.1039/b920572a	journal	January 2010
Evaluation of tip-enhanced Raman spectroscopy for characterizing different virus strains Hermann, Peter; Hermelink, Antje; Lausch, Veronika The Analyst, Vol. 136, Issue 6 https://doi.org/10.1039/c0an00531b	journal	January 2011
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