Mechanistic insights into energy conservation by flavin-based electron bifurcation

Lubner, Carolyn E.; Jennings, David P.; Mulder, David W.; Schut, Gerrit J.; Zadvornyy, Oleg A.; Hoben, John P.; Tokmina-Lukaszewska, Monika; Berry, Luke; Nguyen, Diep M.; Lipscomb, Gina L.; Bothner, Brian; Jones, Anne K.; Miller, Anne-Frances; King, Paul W.; Adams, Michael W. W.; Peters, John W.

doi:10.1038/nchembio.2348

Title: Mechanistic insights into energy conservation by flavin-based electron bifurcation

Journal Article · Mon Apr 10 00:00:00 EDT 2017 · Nature Chemical Biology

DOI: https://doi.org/10.1038/nchembio.2348 · OSTI ID:1358334

Lubner, Carolyn E. ^[1]; Jennings, David P. ^[2]; Mulder, David W. ^[1]; Schut, Gerrit J. ^[3]; Zadvornyy, Oleg A. ^[4]; Hoben, John P. ^[5]; Tokmina-Lukaszewska, Monika ^[4]; Berry, Luke ^[4]; Nguyen, Diep M. ^[3]; Lipscomb, Gina L. ^[3]; Bothner, Brian ^[4]; Jones, Anne K. ^[2];

^[5];

^[1]; Adams, Michael W. W. ^[3]; Peters, John W. ^[6]

National Renewable Energy Lab. (NREL), Golden, CO (United States)
Arizona State Univ., Tempe, AZ (United States)
Univ. of Georgia, Athens, GA (United States)
Montana State Univ., Bozeman, MT (United States)
Univ. of Kentucky, Lexington, KY (United States)
Montana State Univ., Bozeman, MT (United States); Washington State Univ., Pullman, WA (United States)

The recently realized biochemical phenomenon of energy conservation through electron bifurcation provides biology with an elegant means to maximize utilization of metabolic energy. The mechanism of coordinated coupling of exergonic and endergonic oxidation-reduction reactions by a single enzyme complex has been elucidated through optical and paramagnetic spectroscopic studies revealing unprecedented features. Pairs of electrons are bifurcated over more than 1 volt of electrochemical potential by generating a low-potential, highly energetic, unstable flavin semiquinone and directing electron flow to an iron-sulfur cluster with a highly negative potential to overcome the barrier of the endergonic half reaction. As a result, the unprecedented range of thermodynamic driving force that is generated by flavin-based electron bifurcation accounts for unique chemical reactions that are catalyzed by these enzymes.

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Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1358334

Report Number(s):: NREL/JA--2700-67530

Journal Information:: Nature Chemical Biology, Journal Name: Nature Chemical Biology Journal Issue: 6 Vol. 13; ISSN 1552-4450

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Mechanistic insights into energy conservation by flavin-based electron bifurcation

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