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Title: Highly stable multi-anchored magnetic nanoparticles for optical imaging within biofilms

Magnetic nanoparticles are the next tool in medical diagnoses and treatment in many different biomedical applications, including magnetic hyperthermia as alternative treatment for cancer and bacterial infections, as well as the disruption of biofilms. The colloidal stability of the magnetic nanoparticles in a biological environment is crucial for efficient delivery. A surface that can be easily modifiable can also improve the delivery and imaging properties of the magnetic nanoparticle by adding targeting and imaging moieties, providing a platform for additional modification. The strategy presented in this paper includes multiple nitroDOPA anchors for robust binding to the surface tied to the same polymer backbone as multiple poly(ethylene oxide) chains for steric stability. This approach provides biocompatibility and enhanced stability in fetal bovine serum (FBS) and phosphate buffer saline (PBS). As a proof of concept, these polymer-particles complexes were then modified with a near infrared dye and utilized in characterizing the integration of magnetic nanoparticles in biofilms. Finally, the work presented in this manuscript describes the synthesis and characterization of a nontoxic platform for the labeling of near IR-dyes for bioimaging.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [3] ;  [3] ;  [3] ;  [4] ;  [5] ;  [6] ;  [6] ;  [6] ;  [7]
  1. Clemson Univ., SC (United States). Dept. of Materials Science and Engineering. Center for Optical Materials Science and Engineering Technologies (COMSET)
  2. Univ. of Wisconsin, Madison, WI (United States). Dept. of Materials Science and Engineering
  3. Clemson Univ., SC (United States). Dept. of Biological Sciences
  4. Clemson Univ., SC (United States). Dept. of Biological Sciences. Light Imaging Facility
  5. Clemson Univ., SC (United States). Dept. of Biological Sciences. Inst. of Environmental Toxicology
  6. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
  7. Clemson Univ., SC (United States). Dept. of Materials Science and Engineering. Center for Optical Materials Science and Engineering Technologies (COMSET). Inst. of Environmental Toxicology
Publication Date:
Report Number(s):
SAND2016-12686J
Journal ID: ISSN 0021-9797; PII: S0021979715301077
Grant/Contract Number:
AC04-94AL85000; ECC1062873; CMMI-1057633; CMMI-1130819
Type:
Accepted Manuscript
Journal Name:
Journal of Colloid and Interface Science
Additional Journal Information:
Journal Volume: 459; Journal ID: ISSN 0021-9797
Publisher:
Elsevier
Research Org:
Clemson Univ., SC (United States); Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE Office of Science (SC); National Science Foundation (NSF)
Contributing Orgs:
Univ. of Wisconsin, Madison, WI (United States)
Country of Publication:
United States
Language:
English
Subject:
36 MATERIALS SCIENCE; Magnetic nanoparticles; Heterobifunctional; Multi-anchored; Polyethylene oxide; Click chemistry; Biofilms; Imaging; NitroDOPA; Fluorescence
OSTI Identifier:
1356988