skip to main content
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Interactions regulating the head-to-tail directed assembly of biological Janus rods

Abstract

We can generalize the directed, head-to-tail self-assembly of microtubule filaments in the context of Janus colloidal rods. Specifically, their assembly at the tens of micron-length scale involves a careful balance between long-range electrostatic repulsion and short-range attractive forces. We show that the addition of counterion salts increases the rate of directed assembly by screening the electrostatic forces and enhancing the effectiveness of short-range interactions at the microtubule ends.

Authors:
ORCiD logo [1];  [2];  [3];  [4]; ORCiD logo [2]
  1. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). WMD Threats and Aerosol Science Dept.
  2. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies
  3. Sandia National Lab. (SNL-CA), Livermore, CA (United States). Center for Materials Science and Engineering
  4. Sandia National Lab. (SNL-NM), Albuquerque, NM (United States). Center for Integrated Nanotechnologies; Sandia National Lab. (SNL-CA), Livermore, CA (United States). Center for Materials Science and Engineering
Publication Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22); USDOE National Nuclear Security Administration (NNSA)
OSTI Identifier:
1356856
Report Number(s):
SAND2017-4384J
Journal ID: ISSN 1359-7345; CHCOFS; 652784
Grant/Contract Number:  
AC04-94AL85000
Resource Type:
Accepted Manuscript
Journal Name:
ChemComm
Additional Journal Information:
Journal Volume: 53; Journal Issue: 32; Journal ID: ISSN 1359-7345
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Greene, A. C., Bachand, M., Gomez, A., Stevens, M. J., and Bachand, G. D. Interactions regulating the head-to-tail directed assembly of biological Janus rods. United States: N. p., 2017. Web. doi:10.1039/c7cc01566f.
Greene, A. C., Bachand, M., Gomez, A., Stevens, M. J., & Bachand, G. D. Interactions regulating the head-to-tail directed assembly of biological Janus rods. United States. doi:10.1039/c7cc01566f.
Greene, A. C., Bachand, M., Gomez, A., Stevens, M. J., and Bachand, G. D. Fri . "Interactions regulating the head-to-tail directed assembly of biological Janus rods". United States. doi:10.1039/c7cc01566f. https://www.osti.gov/servlets/purl/1356856.
@article{osti_1356856,
title = {Interactions regulating the head-to-tail directed assembly of biological Janus rods},
author = {Greene, A. C. and Bachand, M. and Gomez, A. and Stevens, M. J. and Bachand, G. D.},
abstractNote = {We can generalize the directed, head-to-tail self-assembly of microtubule filaments in the context of Janus colloidal rods. Specifically, their assembly at the tens of micron-length scale involves a careful balance between long-range electrostatic repulsion and short-range attractive forces. We show that the addition of counterion salts increases the rate of directed assembly by screening the electrostatic forces and enhancing the effectiveness of short-range interactions at the microtubule ends.},
doi = {10.1039/c7cc01566f},
journal = {ChemComm},
number = 32,
volume = 53,
place = {United States},
year = {2017},
month = {3}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record

Save / Share:

Works referenced in this record:

Toward Design Rules of Directional Janus Colloidal Assembly
journal, April 2015


Electrostatics of nanosystems: Application to microtubules and the ribosome
journal, August 2001

  • Baker, N. A.; Sept, D.; Joseph, S.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 18, p. 10037-10041
  • DOI: 10.1073/pnas.181342398

Charge-Shielding and the “Paradoxical” Stimulation of Tubulin Polymerization by Guanidine Hydrochloride
journal, January 1996

  • Wolff, J.; Knipling, Leslie; Sackett, Dan L.
  • Biochemistry, Vol. 35, Issue 18
  • DOI: 10.1021/bi9527395

Effective charge saturation in colloidal suspensions
journal, November 2002

  • Bocquet, Lydéric; Trizac, Emmanuel; Aubouy, Miguel
  • The Journal of Chemical Physics, Vol. 117, Issue 17
  • DOI: 10.1063/1.1511507

Molecular Self-Assembly across Multiple Length Scales
journal, June 2007

  • Palermo, Vincenzo; Samorì, Paolo
  • Angewandte Chemie International Edition, Vol. 46, Issue 24
  • DOI: 10.1002/anie.200700416

Janus Particles: Synthesis, Self-Assembly, Physical Properties, and Applications
journal, March 2013

  • Walther, Andreas; Müller, Axel H. E.
  • Chemical Reviews, Vol. 113, Issue 7, p. 5194-5261
  • DOI: 10.1021/cr300089t

End-to-end annealing of microtubules in vitro
journal, February 1986


Direct observation of microtubule treadmilling by electron microscopy
journal, November 1985


Engineering the length distribution of microtubules polymerized in vitro
journal, January 2010

  • Jeune-Smith, Yolaine; Hess, Henry
  • Soft Matter, Vol. 6, Issue 8
  • DOI: 10.1039/b919488f

Supracolloidal Reaction Kinetics of Janus Spheres
journal, January 2011


Polymer physics of the cytoskeleton
journal, October 2011


Directed self-assembly of 1D microtubule nano-arrays
journal, January 2014

  • Bachand, M.; Bouxsein, N. F.; Cheng, S.
  • RSC Adv., Vol. 4, Issue 97
  • DOI: 10.1039/C4RA11765D

Directed Self-Assembly of Nanoparticles
journal, June 2010

  • Grzelczak, Marek; Vermant, Jan; Furst, Eric M.
  • ACS Nano, Vol. 4, Issue 7
  • DOI: 10.1021/nn100869j

The relative contributions of polymer annealing and subunit exchange to microtubule dynamics in vitro
journal, August 1987


Synchrotron X-ray Diffraction Study of Microtubules Buckling and Bundling under Osmotic Stress: A Probe of Interprotofilament Interactions
journal, November 2004


Directed self-assembly of block copolymers: a tutorial review of strategies for enabling nanotechnology with soft matter
journal, January 2014

  • Hu, Hanqiong; Gopinadhan, Manesh; Osuji, Chinedum O.
  • Soft Matter, Vol. 10, Issue 22
  • DOI: 10.1039/c3sm52607k

From The Cover: Higher-order assembly of microtubules by counterions: From hexagonal bundles to living necklaces
journal, November 2004

  • Needleman, D. J.; Ojeda-Lopez, M. A.; Raviv, U.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 46
  • DOI: 10.1073/pnas.0406076101

Cation selective promotion of tubulin polymerization by alkali metal chlorides
journal, October 1996


High-Resolution Model of the Microtubule
journal, January 1999


Self-assembly of anisotropic particles
journal, January 2011

  • Fejer, Szilard N.; Chakrabarti, Dwaipayan; Wales, David J.
  • Soft Matter, Vol. 7, Issue 7
  • DOI: 10.1039/c0sm01289k

Analysis of Tubulin Alpha-1A/1B C-Terminal Tail Post-translational Poly-glutamylation Reveals Novel Modification Sites
journal, January 2012

  • Sahab, Ziad J.; Kirilyuk, Alexander; Zhang, Lihua
  • Journal of Proteome Research, Vol. 11, Issue 3
  • DOI: 10.1021/pr2011044

Directed self-assembly of a colloidal kagome lattice
journal, January 2011

  • Chen, Qian; Bae, Sung Chul; Granick, Steve
  • Nature, Vol. 469, Issue 7330
  • DOI: 10.1038/nature09713

Anisotropy of building blocks and their assembly into complex structures
journal, August 2007

  • Glotzer, Sharon C.; Solomon, Michael J.
  • Nature Materials, Vol. 6, Issue 8, p. 557-562
  • DOI: 10.1038/nmat1949

Single Filament Behavior of Microtubules in the Presence of Added Divalent Counterions
journal, September 2014

  • Bouxsein, Nathan F.; Bachand, George D.
  • Biomacromolecules, Vol. 15, Issue 10
  • DOI: 10.1021/bm500988r

Self-Assembly at All Scales
journal, March 2002

  • Whitesides, George M.; Grzybowski, Bartosz
  • Science, Vol. 295, Issue 5564, p. 2418-2421
  • DOI: 10.1126/science.1070821

Dielectric Measurement of Individual Microtubules Using the Electroorientation Method
journal, May 2006


Self-Assembly of Nanoparticle Amphiphiles with Adaptive Surface Chemistry
journal, September 2014

  • Lee, Hee-Young; Shin, Sun Hae Ra; Drews, Aaron M.
  • ACS Nano, Vol. 8, Issue 10
  • DOI: 10.1021/nn504734v

Ion specific effects in bundling and depolymerization of taxol-stabilized microtubules
journal, January 2013

  • Needleman, Daniel J.; Ojeda-Lopez, Miguel A.; Raviv, Uri
  • Faraday Discussions, Vol. 166
  • DOI: 10.1039/c3fd00063j