Double-flow focused liquid injector for efficient serial femtosecond crystallography

Oberthuer, Dominik; Knoška, Juraj; Wiedorn, Max O.; Beyerlein, Kenneth R.; Bushnell, David A.; Kovaleva, Elena G.; Heymann, Michael; Gumprecht, Lars; Kirian, Richard A.; Barty, Anton; Mariani, Valerio; Tolstikova, Aleksandra; Adriano, Luigi; Awel, Salah; Barthelmess, Miriam; Dörner, Katerina; Xavier, P. Lourdu; Yefanov, Oleksandr; James, Daniel R.; Nelson, Garrett; Wang, Dingjie; Calvey, George; Chen, Yujie; Schmidt, Andrea; Szczepek, Michael; Frielingsdorf, Stefan; Lenz, Oliver; Snell, Edward; Robinson, Philip J.; Šarler, Božidar; Belšak, Grega; Maček, Marjan; Wilde, Fabian; Aquila, Andrew; Boutet, Sébastien; Liang, Mengning; Hunter, Mark S.; Scheerer, Patrick; Lipscomb, John D.; Weierstall, Uwe; Kornberg, Roger D.; Spence, John C. H.; Pollack, Lois; Chapman, Henry N.; Bajt, Saša

doi:10.1038/srep44628

Title: Double-flow focused liquid injector for efficient serial femtosecond crystallography

Journal Article · Thu Mar 16 00:00:00 UTC 2017 · Scientific Reports

DOI: https://doi.org/10.1038/srep44628 · OSTI ID:1352549

Oberthuer, Dominik ^[1]; Knoška, Juraj ^[2]; Wiedorn, Max O. ^[2]; Beyerlein, Kenneth R. ^[1]; Bushnell, David A. ^[3]; Kovaleva, Elena G. ^[4]; Heymann, Michael ^[5]; Gumprecht, Lars ^[1]; Kirian, Richard A. ^[6]; Barty, Anton ^[1]; Mariani, Valerio ^[1]; Tolstikova, Aleksandra ^[2]; Adriano, Luigi ^[1]; Awel, Salah ^[7]; Barthelmess, Miriam ^[1]; Dörner, Katerina ^[8]; Xavier, P. Lourdu ^[9]; Yefanov, Oleksandr ^[1]; James, Daniel R. ^[10]; Nelson, Garrett ^[6] more »

Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
Stanford Univ., Stanford, CA (United States)
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Max-Planck-Institute for Biochemistry, Munich (Germany)
Arizona State Univ., Tempe, AZ (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Centre for Ultrafast Imaging, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); European XFEL GmbH, Hamburg (Germany)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
Arizona State Univ., Tempe, AZ (United States); Paul Scherrer Inst. (PSI), Villigen (Switzerland)
Cornell Univ., Ithaca, NY (United States)
Charite - Univ. Berlin, Berlin (Germany)
Technische Univ. Berlin, Berlin (Germany)
Hauptman-Woodward Medical Research Institute, Buffalo, NY (United States)
Univ. of Nova Gorica, Nova Gorica (Slovenia); Institute of Metals and Technology, Ljubljana (Slovenia)
Institute of Metals and Technology, Ljubljana (Slovenia)
Helmholtz-Zentrum Geesthacht, Geesthacht (Germany)
Univ. of Minnesota, Minneapolis, MN (United States)
Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Univ. of Minnesota, Minneapolis, MN (United States)

Serial femtosecond crystallography requires reliable and efficient delivery of fresh crystals across the beam of an X-ray free-electron laser over the course of an experiment. We introduce a double-flow focusing nozzle to meet this challenge, with significantly reduced sample consumption, while improving jet stability over previous generations of nozzles. We demonstrate its use to determine the first room-temperature structure of RNA polymerase II at high resolution, revealing new structural details. Furthermore, the double flow-focusing nozzles were successfully tested with three other protein samples and the first room temperature structure of an extradiol ring-cleaving dioxygenase was solved by utilizing the improved operation and characteristics of these devices.

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Research Organization:: SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-76SF00515

OSTI ID:: 1352549

Journal Information:: Scientific Reports, Journal Name: Scientific Reports Vol. 7; ISSN 2045-2322

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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