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Title: Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles

Abstract

Low- and high-resolution models describing the internal transbilayer structure of asymmetric lipid vesicles have been developed. These models can be used for the joint analysis of small-angle neutron and X-ray scattering data. The models describe the underlying scattering length density/electron density profiles either in terms of slabs or through the so-called scattering density profile, previously applied to symmetric lipid vesicles. Both models yield structural details of asymmetric membranes, such as the individual area per lipid, and the hydrocarbon thickness of the inner and outer bilayer leaflets. The scattering density profile model, however, comes at a cost of increased computational effort but results in greater structural resolution, showing a slightly lower packing of lipids in the outer bilayer leaflet of ~120 nm diameter palmitoyloleoyl phosphatidylcholine (POPC) vesicles, compared to the inner leaflet. Here, analysis of asymmetric dipalmitoyl phosphatidylcholine/POPC vesicles did not reveal evidence of transbilayer coupling between the inner and outer leaflets at 323 K,i.e.above the melting transition temperature of the two lipids.

Authors:
 [1];  [2];  [1];  [3];  [4]; ORCiD logo [1]
+ Show Author Affiliations
  1. Univ. of Graz (Austria); BioTechMed-Graz, Graz (Austria)
  2. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Univ. of Graz (Austria); BioTechMed-Graz (Austria)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1351774
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Applied Crystallography (Online)
Additional Journal Information:
Journal Name: Journal of Applied Crystallography (Online); Journal Volume: 50; Journal Issue: 2; Journal ID: ISSN 1600-5767
Publisher:
International Union of Crystallography
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Eicher, Barbara, Heberle, Frederick A., Marquardt, Drew T., Rechberger, Gerald N., Katsaras, John, and Pabst, Georg. Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles. United States: N. p., 2017. Web. doi:10.1107/S1600576717000656.
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Eicher, Barbara, Heberle, Frederick A., Marquardt, Drew T., Rechberger, Gerald N., Katsaras, John, & Pabst, Georg. Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles. United States. https://doi.org/10.1107/S1600576717000656
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Eicher, Barbara, Heberle, Frederick A., Marquardt, Drew T., Rechberger, Gerald N., Katsaras, John, and Pabst, Georg. Tue . "Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles". United States. https://doi.org/10.1107/S1600576717000656. https://www.osti.gov/servlets/purl/1351774.
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@article{osti_1351774,
title = {Joint small-angle X-ray and neutron scattering data analysis of asymmetric lipid vesicles},
author = {Eicher, Barbara and Heberle, Frederick A. and Marquardt, Drew T. and Rechberger, Gerald N. and Katsaras, John and Pabst, Georg},
abstractNote = {Low- and high-resolution models describing the internal transbilayer structure of asymmetric lipid vesicles have been developed. These models can be used for the joint analysis of small-angle neutron and X-ray scattering data. The models describe the underlying scattering length density/electron density profiles either in terms of slabs or through the so-called scattering density profile, previously applied to symmetric lipid vesicles. Both models yield structural details of asymmetric membranes, such as the individual area per lipid, and the hydrocarbon thickness of the inner and outer bilayer leaflets. The scattering density profile model, however, comes at a cost of increased computational effort but results in greater structural resolution, showing a slightly lower packing of lipids in the outer bilayer leaflet of ~120 nm diameter palmitoyloleoyl phosphatidylcholine (POPC) vesicles, compared to the inner leaflet. Here, analysis of asymmetric dipalmitoyl phosphatidylcholine/POPC vesicles did not reveal evidence of transbilayer coupling between the inner and outer leaflets at 323 K,i.e.above the melting transition temperature of the two lipids.},
doi = {10.1107/S1600576717000656},
journal = {Journal of Applied Crystallography (Online)},
number = 2,
volume = 50,
place = {United States},
year = {Tue Feb 28 00:00:00 EST 2017},
month = {Tue Feb 28 00:00:00 EST 2017}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1107/S1600576717000656
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Cited by: 37 works
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Figures / Tables:

Figure 1 Figure 1: Schematic illustration of the four-slab model. Upper, space-filling representation of an asymmetric bilayer. Lower, the neutron scattering length density (NSLD) profile across the bilayer is obtained by averaging the composition of each slab.
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    Works referencing / citing this record:

    Complex biomembrane mimetics on the sub-nanometer scale
    journal, July 2017

    Preparation of asymmetric phospholipid vesicles for use as cell membrane models
    journal, September 2018

    Transverse lipid organization dictates bending fluctuations in model plasma membranes
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    • Journal of Applied Crystallography, Vol. 53, Issue 1
    • DOI: 10.1107/s1600576719015656
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