Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment
Abstract
Quantifying the contribution of marine microorganisms to carbon and nitrogen cycles and their response to predicted ocean warming is one of the main challenges of microbial oceanography. Here we present a single-cell NanoSIMS isotope analysis to quantify C and N uptake by free-living and attached phytoplankton and heterotrophic bacteria, and their response to short-term experimental warming of 4 °C. Elevated temperature increased total C fixation by over 50%, a small but significant fraction of which was transferred to heterotrophs within 12 h. Cell-to-cell attachment doubled the secondary C uptake by heterotrophic bacteria and increased secondary N incorporation by autotrophs by 68%. Warming also increased the abundance of phytoplankton with attached heterotrophs by 80%, and promoted C transfer from phytoplankton to bacteria by 17% and N transfer from bacteria to phytoplankton by 50%. Lastly, our results indicate that phytoplankton-bacteria attachment provides an ecological advantage for nutrient incorporation, suggesting a mutualistic relationship that appears to be enhanced by temperature increases.
- Authors:
-
- Centro Oceanografico de Gijon/Xixon, Asturias (Spain)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
- Centro Oceanografico de Gijon/Xixon, Asturias (Spain); AZTI, Bizkaia (Spain)
- Centro Oceanografico de Gijon/Xixon, Asturias (Spain); King Abdullah Univ. of Science and Technology, Thuwal (Saudi Arabia)
- Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Oregon State Univ., Corvallis, OR (United States)
- Publication Date:
- Research Org.:
- Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1351131
- Report Number(s):
- LLNL-JRNL-677811
Journal ID: ISSN 1751-7362
- Grant/Contract Number:
- AC52-07NA27344
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 3; Journal ID: ISSN 1751-7362
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 54 ENVIRONMENTAL SCIENCES
Citation Formats
Arandia-Gorostidi, Nestor, Weber, Peter K., Alonso-Sáez, Laura, Morán, Xosé Anxelu G., and Mayali, Xavier. Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment. United States: N. p., 2016.
Web. doi:10.1038/ismej.2016.156.
Arandia-Gorostidi, Nestor, Weber, Peter K., Alonso-Sáez, Laura, Morán, Xosé Anxelu G., & Mayali, Xavier. Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment. United States. https://doi.org/10.1038/ismej.2016.156
Arandia-Gorostidi, Nestor, Weber, Peter K., Alonso-Sáez, Laura, Morán, Xosé Anxelu G., and Mayali, Xavier. Tue .
"Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment". United States. https://doi.org/10.1038/ismej.2016.156. https://www.osti.gov/servlets/purl/1351131.
@article{osti_1351131,
title = {Elevated temperature increases carbon and nitrogen fluxes between phytoplankton and heterotrophic bacteria through physical attachment},
author = {Arandia-Gorostidi, Nestor and Weber, Peter K. and Alonso-Sáez, Laura and Morán, Xosé Anxelu G. and Mayali, Xavier},
abstractNote = {Quantifying the contribution of marine microorganisms to carbon and nitrogen cycles and their response to predicted ocean warming is one of the main challenges of microbial oceanography. Here we present a single-cell NanoSIMS isotope analysis to quantify C and N uptake by free-living and attached phytoplankton and heterotrophic bacteria, and their response to short-term experimental warming of 4 °C. Elevated temperature increased total C fixation by over 50%, a small but significant fraction of which was transferred to heterotrophs within 12 h. Cell-to-cell attachment doubled the secondary C uptake by heterotrophic bacteria and increased secondary N incorporation by autotrophs by 68%. Warming also increased the abundance of phytoplankton with attached heterotrophs by 80%, and promoted C transfer from phytoplankton to bacteria by 17% and N transfer from bacteria to phytoplankton by 50%. Lastly, our results indicate that phytoplankton-bacteria attachment provides an ecological advantage for nutrient incorporation, suggesting a mutualistic relationship that appears to be enhanced by temperature increases.},
doi = {10.1038/ismej.2016.156},
journal = {The ISME Journal},
number = 3,
volume = 11,
place = {United States},
year = {Tue Dec 06 00:00:00 EST 2016},
month = {Tue Dec 06 00:00:00 EST 2016}
}
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