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Title: Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre

Abstract

Photosynthesis uses a limited range of the solar spectrum, so enhancing spectral coverage could improve the efficiency of light capture. Here, we show that a hybrid reaction centre (RC)/yellow fluorescent protein (YFP) complex accelerates photosynthetic growth in the bacterium Rhodobacter sphaeroides. The structure of the RC/YFP-light-harvesting 1 (LH1) complex shows the position of YFP attachment to the RC-H subunit, on the cytoplasmic side of the RC complex. Fluorescence lifetime microscopy of whole cells and ultrafast transient absorption spectroscopy of purified RC/YFP complexes show that the YFP–RC intermolecular distance and spectral overlap between the emission of YFP and the visible-region (Q X) absorption bands of the RC allow energy transfer via a Fo¨rster mechanism, with an efficiency of 40±10%. Finally, this proof-of-principle study demonstrates the feasibility of increasing spectral coverage for harvesting light using non-native genetically-encoded light-absorbers, thereby augmenting energy transfer and trapping in photosynthesis.

Authors:
ORCiD logo [1];  [2];  [1];  [1];  [2];  [1];  [1];  [1];  [2]; ORCiD logo [2];  [1];  [2];  [2]; ORCiD logo [1]
  1. Univ. of Sheffield, Sheffield (United Kingdom)
  2. Washington Univ., St. Louis, MO (United States)
Publication Date:
Research Org.:
Washington Univ., St. Louis, MO (United States); Energy Frontier Research Centers (EFRC) (United States). Photosynthetic Antenna Research Center (PARC)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1345997
Grant/Contract Number:  
SC0001035
Resource Type:
Accepted Manuscript
Journal Name:
Nature Communications
Additional Journal Information:
Journal Volume: 8; Journal ID: ISSN 2041-1723
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; bioenergetics; devices for energy harvesting; photosynthesis; synthetic biology

Citation Formats

Grayson, Katie J., Faries, Kaitlyn M., Huang, Xia, Qian, Pu, Dilbeck, Preston, Martin, Elizabeth C., Hitchcock, Andrew, Vasilev, Cvetelin, Yuen, Jonathan M., Niedzwiedzki, Dariusz M., Leggett, Graham J., Holten, Dewey, Kirmaier, Christine, and Hunter, C. Neil. Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre. United States: N. p., 2017. Web. doi:10.1038/ncomms13972.
Grayson, Katie J., Faries, Kaitlyn M., Huang, Xia, Qian, Pu, Dilbeck, Preston, Martin, Elizabeth C., Hitchcock, Andrew, Vasilev, Cvetelin, Yuen, Jonathan M., Niedzwiedzki, Dariusz M., Leggett, Graham J., Holten, Dewey, Kirmaier, Christine, & Hunter, C. Neil. Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre. United States. doi:10.1038/ncomms13972.
Grayson, Katie J., Faries, Kaitlyn M., Huang, Xia, Qian, Pu, Dilbeck, Preston, Martin, Elizabeth C., Hitchcock, Andrew, Vasilev, Cvetelin, Yuen, Jonathan M., Niedzwiedzki, Dariusz M., Leggett, Graham J., Holten, Dewey, Kirmaier, Christine, and Hunter, C. Neil. Thu . "Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre". United States. doi:10.1038/ncomms13972. https://www.osti.gov/servlets/purl/1345997.
@article{osti_1345997,
title = {Augmenting light coverage for photosynthesis through YFP-enhanced charge separation at the Rhodobacter sphaeroides reaction centre},
author = {Grayson, Katie J. and Faries, Kaitlyn M. and Huang, Xia and Qian, Pu and Dilbeck, Preston and Martin, Elizabeth C. and Hitchcock, Andrew and Vasilev, Cvetelin and Yuen, Jonathan M. and Niedzwiedzki, Dariusz M. and Leggett, Graham J. and Holten, Dewey and Kirmaier, Christine and Hunter, C. Neil},
abstractNote = {Photosynthesis uses a limited range of the solar spectrum, so enhancing spectral coverage could improve the efficiency of light capture. Here, we show that a hybrid reaction centre (RC)/yellow fluorescent protein (YFP) complex accelerates photosynthetic growth in the bacterium Rhodobacter sphaeroides. The structure of the RC/YFP-light-harvesting 1 (LH1) complex shows the position of YFP attachment to the RC-H subunit, on the cytoplasmic side of the RC complex. Fluorescence lifetime microscopy of whole cells and ultrafast transient absorption spectroscopy of purified RC/YFP complexes show that the YFP–RC intermolecular distance and spectral overlap between the emission of YFP and the visible-region (QX) absorption bands of the RC allow energy transfer via a Fo¨rster mechanism, with an efficiency of 40±10%. Finally, this proof-of-principle study demonstrates the feasibility of increasing spectral coverage for harvesting light using non-native genetically-encoded light-absorbers, thereby augmenting energy transfer and trapping in photosynthesis.},
doi = {10.1038/ncomms13972},
journal = {Nature Communications},
number = ,
volume = 8,
place = {United States},
year = {2017},
month = {1}
}

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