Characterization of microsatellite loci for an Australian epiphytic orchid, Dendrobium calamiforme , using Illumina sequencing

Journal Article · Fri Jun 05 00:00:00 EDT 2015 · Applications in Plant Science

DOI: https://doi.org/10.3732/apps.1500016 · OSTI ID:1787080

Trapnell, Dorset W.; Beasley, Rochelle R.; Lance, Stacey L.; Field, Ashley R.; Jones, Kenneth L.

Premise of the study: Microsatellite loci were developed for the epiphytic pencil orchid Dendrobium calamiforme for population genetic and phylogeographic investigation of this Australian taxon. Methods and Results: Nineteen microsatellite loci were identified from an Illumina paired‐end shotgun library of D. calamiforme . Polymorphism and genetic diversity were assessed in 24 individuals from five populations separated by a maximum distance of ∼80 km. All loci were polymorphic with two to 14 alleles per locus, expected heterozygosity ranging from 0.486 to 0.902, and probability of identity values ranging from 0.018 to 0.380. Conclusions: These novel markers will serve as valuable tools for investigation of levels of genetic diversity as well as patterns of gene flow, genetic structure, and phylogeographic history.

• Sponsoring Organization: USDOE
• Grant/Contract Number: FC09-07SR22506
• OSTI ID: 1787080
• Journal Information: Applications in Plant Science, Journal Name: Applications in Plant Science Journal Issue: 6 Vol. 3; ISSN 2168-0450
• Publisher: Wiley Blackwell (John Wiley & Sons)Copyright Statement
• Country of Publication: United States
• Language: English