Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges
Abstract
In this paper, a facile method was developed to purify 2-[18F]fluoroethyl azide ([18F]FEA) using a C18 cartridge and an Oasis® HLB cartridge in series, in which [18F]FEA was exclusively trapped on the HLB cartridge. [18F]FEA can be eluted for reactions in solution; alternatively click labeling can be carried out on the HLB cartridge itself by loading an alkyne substrate and copper (I) catalyst dissolved in DMF onto the cartridge. Finally, this solid phase extraction methodology for purification and click labeling with [18F]FEA, either in solution or on the cartridge, is safe, simple, reproducible in high yield, and compatible with automated synthesis of 18F-labeled PET tracers.
- Authors:
-
- Washington Univ. School of Medicine, St. Louis, MO (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States); Univ. of Pennsylvania, Philadelphia, PA (United States)
- Univ. of Illinois, Urbana, IL (United States)
- Publication Date:
- Research Org.:
- Washington Univ., St. Louis, MO (United States). School of Medicine; Univ. of Illinois at Urbana-Champaign, IL (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1344513
- Alternate Identifier(s):
- OSTI ID: 1366319
- Grant/Contract Number:
- SC0005434; SC0002032
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Tetrahedron Letters
- Additional Journal Information:
- Journal Volume: 56; Journal Issue: 7; Journal ID: ISSN 0040-4039
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; Fluorine-18; radiolabeling; click chemistry; 2-Fluoroethyl azide; positron emission tomography; solid phase extraction
Citation Formats
Zhou, Dong, Chu, Wenhua, Peng, Xin, McConathy, Jonathan, Mach, Robert H., and Katzenellenbogen, John A. Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges. United States: N. p., 2014.
Web. doi:10.1016/j.tetlet.2014.10.126.
Zhou, Dong, Chu, Wenhua, Peng, Xin, McConathy, Jonathan, Mach, Robert H., & Katzenellenbogen, John A. Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges. United States. https://doi.org/10.1016/j.tetlet.2014.10.126
Zhou, Dong, Chu, Wenhua, Peng, Xin, McConathy, Jonathan, Mach, Robert H., and Katzenellenbogen, John A. Tue .
"Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges". United States. https://doi.org/10.1016/j.tetlet.2014.10.126. https://www.osti.gov/servlets/purl/1344513.
@article{osti_1344513,
title = {Facile purification and click labeling with 2-[18F]fluoroethyl azide using solid phase extraction cartridges},
author = {Zhou, Dong and Chu, Wenhua and Peng, Xin and McConathy, Jonathan and Mach, Robert H. and Katzenellenbogen, John A.},
abstractNote = {In this paper, a facile method was developed to purify 2-[18F]fluoroethyl azide ([18F]FEA) using a C18 cartridge and an Oasis® HLB cartridge in series, in which [18F]FEA was exclusively trapped on the HLB cartridge. [18F]FEA can be eluted for reactions in solution; alternatively click labeling can be carried out on the HLB cartridge itself by loading an alkyne substrate and copper (I) catalyst dissolved in DMF onto the cartridge. Finally, this solid phase extraction methodology for purification and click labeling with [18F]FEA, either in solution or on the cartridge, is safe, simple, reproducible in high yield, and compatible with automated synthesis of 18F-labeled PET tracers.},
doi = {10.1016/j.tetlet.2014.10.126},
journal = {Tetrahedron Letters},
number = 7,
volume = 56,
place = {United States},
year = {Tue Nov 04 00:00:00 EST 2014},
month = {Tue Nov 04 00:00:00 EST 2014}
}
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- Reaction Chemistry & Engineering, Vol. 4, Issue 3
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journal, May 2017
- Choi, Hans; Shirley, Harry J.; Hume, Paul A.
- Angewandte Chemie International Edition, Vol. 56, Issue 26
Fluorine-18 labelled building blocks for PET tracer synthesis
journal, January 2017
- van der Born, Dion; Pees, Anna; Poot, Alex J.
- Chem. Soc. Rev., Vol. 46, Issue 15
Unexpected Direct Synthesis of N -Vinyl Amides through Vinyl Azide-Enolate [3+2] Cycloaddition
journal, May 2017
- Choi, Hans; Shirley, Harry J.; Hume, Paul A.
- Angewandte Chemie, Vol. 129, Issue 26