skip to main content

DOE PAGESDOE PAGES

Title: An in-depth understanding of biomass recalcitrance using natural poplar variants as the feedstock

Here, in an effort to better understand the biomass recalcitrance, six natural poplar variants were selected as feedstocks based on previous sugar release analysis. Compositional analysis and physicochemical characterizations of these poplars were performed and the correlations between these physicochemical properties and enzymatic hydrolysis yield were investigated. Gel permeation chromatography (GPC) and 13C solid state NMR were used to determine the degree of polymerization (DP) and crystallinity index (CrI) of cellulose, and the results along with the sugar release study indicated that cellulose DP likely played a more important role in enzymatic hydrolysis. Simons’ stain revealed that the accessible surface area of substrate significantly varied among these variants from 17.3 to 33.2 mg g$$–1\atop{biomass}$$ as reflected by dye adsorption, and cellulose accessibility was shown as one of the major factors governing substrates digestibility. HSQC and 31P NMR analysis detailed the structural features of poplar lignin variants. Overall, cellulose relevant factors appeared to have a stronger correlation with glucose release, if any, than lignin structural features. Lignin structural features, such as a phenolic hydroxyl group and the ratio of syringyl and guaiacyl (S/G), were found to have a more convincing impact on xylose release. Low lignin content, low cellulose DP, and high cellulose accessibility generally favor enzymatic hydrolysis; however, recalcitrance cannot be simply judged on any single substrate factor.
Authors:
 [1] ;  [2] ;  [2] ;  [2] ;  [3] ;  [3] ;  [4] ;  [4] ;  [2] ;  [2] ;  [2] ; ORCiD logo [5]
  1. Univ. of Tennessee, Knoxville, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  3. Georgia Inst. of Technology, Atlanta, GA (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  5. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. Tennessee Institute of Agriculture, Knoxville, TN (United States)
Publication Date:
Report Number(s):
NREL/JA-5100-67897
Journal ID: ISSN 1864-5631
Grant/Contract Number:
AC05-00OR22725; AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
ChemSusChem
Additional Journal Information:
Journal Volume: 9; Journal Issue: 00; Journal ID: ISSN 1864-5631
Publisher:
ChemPubSoc Europe
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; biomass recalcitrance; cellulose; crystallinity; degree of polymerization; lignin
OSTI Identifier:
1343083
Alternate Identifier(s):
OSTI ID: 1337507

Meng, Xianzhi, Pu, Yunqiao, Yoo, Chang Geun, Li, Mi, Bali, Garima, Park, Doh -Yeon, Gjersing, Erica, Davis, Mark F., Muchero, Wellington, Tuskan, Gerald A., Tschaplinski, Timothy J., and Ragauskas, Arthur J.. An in-depth understanding of biomass recalcitrance using natural poplar variants as the feedstock. United States: N. p., Web. doi:10.1002/cssc.201601303.
Meng, Xianzhi, Pu, Yunqiao, Yoo, Chang Geun, Li, Mi, Bali, Garima, Park, Doh -Yeon, Gjersing, Erica, Davis, Mark F., Muchero, Wellington, Tuskan, Gerald A., Tschaplinski, Timothy J., & Ragauskas, Arthur J.. An in-depth understanding of biomass recalcitrance using natural poplar variants as the feedstock. United States. doi:10.1002/cssc.201601303.
Meng, Xianzhi, Pu, Yunqiao, Yoo, Chang Geun, Li, Mi, Bali, Garima, Park, Doh -Yeon, Gjersing, Erica, Davis, Mark F., Muchero, Wellington, Tuskan, Gerald A., Tschaplinski, Timothy J., and Ragauskas, Arthur J.. 2016. "An in-depth understanding of biomass recalcitrance using natural poplar variants as the feedstock". United States. doi:10.1002/cssc.201601303. https://www.osti.gov/servlets/purl/1343083.
@article{osti_1343083,
title = {An in-depth understanding of biomass recalcitrance using natural poplar variants as the feedstock},
author = {Meng, Xianzhi and Pu, Yunqiao and Yoo, Chang Geun and Li, Mi and Bali, Garima and Park, Doh -Yeon and Gjersing, Erica and Davis, Mark F. and Muchero, Wellington and Tuskan, Gerald A. and Tschaplinski, Timothy J. and Ragauskas, Arthur J.},
abstractNote = {Here, in an effort to better understand the biomass recalcitrance, six natural poplar variants were selected as feedstocks based on previous sugar release analysis. Compositional analysis and physicochemical characterizations of these poplars were performed and the correlations between these physicochemical properties and enzymatic hydrolysis yield were investigated. Gel permeation chromatography (GPC) and 13C solid state NMR were used to determine the degree of polymerization (DP) and crystallinity index (CrI) of cellulose, and the results along with the sugar release study indicated that cellulose DP likely played a more important role in enzymatic hydrolysis. Simons’ stain revealed that the accessible surface area of substrate significantly varied among these variants from 17.3 to 33.2 mg g$–1\atop{biomass}$ as reflected by dye adsorption, and cellulose accessibility was shown as one of the major factors governing substrates digestibility. HSQC and 31P NMR analysis detailed the structural features of poplar lignin variants. Overall, cellulose relevant factors appeared to have a stronger correlation with glucose release, if any, than lignin structural features. Lignin structural features, such as a phenolic hydroxyl group and the ratio of syringyl and guaiacyl (S/G), were found to have a more convincing impact on xylose release. Low lignin content, low cellulose DP, and high cellulose accessibility generally favor enzymatic hydrolysis; however, recalcitrance cannot be simply judged on any single substrate factor.},
doi = {10.1002/cssc.201601303},
journal = {ChemSusChem},
number = 00,
volume = 9,
place = {United States},
year = {2016},
month = {12}
}

Works referenced in this record:

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

Biomass Recalcitrance: Engineering Plants and Enzymes for Biofuels Production
journal, February 2007
  • Himmel, M. E.; Ding, S.-Y.; Johnson, D. K.
  • Science, Vol. 315, Issue 5813, p. 804-807
  • DOI: 10.1126/science.1137016

Inhibition of cellulase, xylanase and β-glucosidase activities by softwood lignin preparations
journal, September 2006

The Path Forward for Biofuels and Biomaterials
journal, January 2006
  • Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
  • Science, Vol. 311, Issue 5760, p. 484-489
  • DOI: 10.1126/science.1114736

The effect of initial pore volume and lignin content on the enzymatic hydrolysis of softwoods
journal, May 1998
  • Mooney, Caitriona A.; Mansfield, Shawn D.; Touhy, Maria G.
  • Bioresource Technology, Vol. 64, Issue 2, p. 113-119
  • DOI: 10.1016/S0960-8524(97)00181-8

Toward an aggregated understanding of enzymatic hydrolysis of cellulose: Noncomplexed cellulase systems
journal, November 2004
  • Zhang, Yi-Heng Percival; Lynd, Lee R.
  • Biotechnology and Bioengineering, Vol. 88, Issue 7, p. 797-824
  • DOI: 10.1002/bit.20282