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Title: Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids

Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. As a result, it was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.
Authors:
ORCiD logo [1] ; ORCiD logo [1] ;  [2] ;  [1] ;  [3] ;  [3] ; ORCiD logo [1]
  1. Univ. of Colorado, Boulder, CO (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. Waters Corp., Wilmslow (United Kingdom)
Publication Date:
Report Number(s):
NREL/JA-5K00-67567
Journal ID: ISSN 0003-2700
Grant/Contract Number:
AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
Analytical Chemistry
Additional Journal Information:
Journal Volume: 89; Journal Issue: 1; Journal ID: ISSN 0003-2700
Publisher:
American Chemical Society (ACS)
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; mass spectrometry; phospholipids; biology
OSTI Identifier:
1339606

Berry, Karin A. Zemski, Barkley, Robert M., Berry, Joseph J., Hankin, Joseph A., Hoyes, Emmy, Brown, Jeffery M., and Murphy, Robert C.. Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids. United States: N. p., Web. doi:10.1021/acs.analchem.6b04047.
Berry, Karin A. Zemski, Barkley, Robert M., Berry, Joseph J., Hankin, Joseph A., Hoyes, Emmy, Brown, Jeffery M., & Murphy, Robert C.. Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids. United States. doi:10.1021/acs.analchem.6b04047.
Berry, Karin A. Zemski, Barkley, Robert M., Berry, Joseph J., Hankin, Joseph A., Hoyes, Emmy, Brown, Jeffery M., and Murphy, Robert C.. 2016. "Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids". United States. doi:10.1021/acs.analchem.6b04047. https://www.osti.gov/servlets/purl/1339606.
@article{osti_1339606,
title = {Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids},
author = {Berry, Karin A. Zemski and Barkley, Robert M. and Berry, Joseph J. and Hankin, Joseph A. and Hoyes, Emmy and Brown, Jeffery M. and Murphy, Robert C.},
abstractNote = {Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. As a result, it was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.},
doi = {10.1021/acs.analchem.6b04047},
journal = {Analytical Chemistry},
number = 1,
volume = 89,
place = {United States},
year = {2016},
month = {11}
}