Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids

Berry, Karin A. Zemski; Barkley, Robert M.; Berry, Joseph J.; Hankin, Joseph A.; Hoyes, Emmy; Brown, Jeffery M.; Murphy, Robert C.

doi:10.1021/acs.analchem.6b04047

Title: Tandem mass spectrometry in combination with product ion mobility for the identification of phospholipids

Journal Article · Mon Nov 28 23:00:00 EST 2016 · Analytical Chemistry

DOI: https://doi.org/10.1021/acs.analchem.6b04047 · OSTI ID:1339606

^[1];

^[1]; Berry, Joseph J. ^[2]; Hankin, Joseph A. ^[1]; Hoyes, Emmy ^[3]; Brown, Jeffery M. ^[3];

^[1]

Univ. of Colorado, Boulder, CO (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Waters Corp., Wilmslow (United Kingdom)

Concerted tandem and traveling wave ion mobility mass spectrometry (CTS analysis) is a unique method that results in a four-dimensional data set including nominal precursor ion mass, product ion mobility, accurate mass of product ion, and ion abundance. This nontargeted lipidomics CTS approach was applied in both positive- and negative-ion mode to phospholipids present in human serum, and the data set was used to evaluate the value of product ion mobility in identifying lipids in a complex mixture. As a result, it was determined that the combination of diagnostic product ions and unique collisional cross-section values of product ions is a powerful tool in the structural identification of lipids in a complex biological sample.

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Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1339606

Report Number(s):: NREL/JA--5K00-67567

Journal Information:: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 1 Vol. 89; ISSN 0003-2700

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

References (26)

Electrospray mass spectrometry of phospholipids Pulfer, Melissa; Murphy, Robert C. Mass Spectrometry Reviews, Vol. 22, Issue 5 https://doi.org/10.1002/mas.10061	journal	August 2003
Multi-dimensional mass spectrometry-based shotgun lipidomics and novel strategies for lipidomic analyses Han, Xianlin; Yang, Kui; Gross, Richard W. Mass Spectrometry Reviews, Vol. 31, Issue 1 https://doi.org/10.1002/mas.20342	journal	July 2011
Applications of ion-mobility mass spectrometry for lipid analysis Paglia, Giuseppe; Kliman, Michal; Claude, Emmanuelle Analytical and Bioanalytical Chemistry, Vol. 407, Issue 17 https://doi.org/10.1007/s00216-015-8664-8	journal	April 2015
Lipid glycation and protein glycation in diabetes and atherosclerosis Miyazawa, Teruo; Nakagawa, Kiyotaka; Shimasaki, Satoko Amino Acids, Vol. 42, Issue 4 https://doi.org/10.1007/s00726-010-0772-3	journal	October 2010
Ion mobility mass spectrometry enhances low-abundance species detection in untargeted lipidomics Basit, Abdul; Pontis, Silvia; Piomelli, Daniele Metabolomics, Vol. 12, Issue 3 https://doi.org/10.1007/s11306-016-0971-3	journal	February 2016
Separation and Classification of Lipids Using Differential Ion Mobility Spectrometry Shvartsburg, Alexandre A.; Isaac, Giorgis; Leveque, Nathalie Journal of The American Society for Mass Spectrometry, Vol. 22, Issue 7 https://doi.org/10.1007/s13361-011-0114-z	journal	April 2011
Localization of Fatty Acyl and Double Bond Positions in Phosphatidylcholines Using a Dual Stage CID Fragmentation Coupled with Ion Mobility Mass Spectrometry Castro-Perez, Jose; Roddy, Thomas P.; Nibbering, Nico M. M. Journal of The American Society for Mass Spectrometry, Vol. 22, Issue 9 https://doi.org/10.1007/s13361-011-0172-2	journal	June 2011
Charge-remote and charge-driven fragmentation processes in diacyl glycerophosphoethanolamine upon low-energy collisional activation: A mechanistic proposal Hsu, Fong-Fu; Turk, J. Journal of the American Society for Mass Spectrometry, Vol. 11, Issue 10 https://doi.org/10.1016/S1044-0305(00)00159-8	journal	October 2000
Characterization of phosphatidylinositol, phosphatidylinositol-4-phosphate, and phosphatidylinositol-4,5-bisphosphate by electrospray ionization tandem mass spectrometry: A mechanistic study Hsu, Fong-Fu; Turk, John Journal of the American Society for Mass Spectrometry, Vol. 11, Issue 11 https://doi.org/10.1016/S1044-0305(00)00172-0	journal	November 2000
Analysis of mammalian sphingolipids by liquid chromatography tandem mass spectrometry (LC-MS/MS) and tissue imaging mass spectrometry (TIMS) Sullards, M. Cameron; Liu, Ying; Chen, Yanfeng Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1811, Issue 11 https://doi.org/10.1016/j.bbalip.2011.06.027	journal	November 2011
Electrospray ionization tandem mass spectrometry of glycerophosphoethanolamine plasmalogen phospholipids Berry, Karin A. Zemski; Murphy, Robert C. Journal of the American Society for Mass Spectrometry, Vol. 15, Issue 10 https://doi.org/10.1016/j.jasms.2004.07.009	journal	October 2004
Differential Mobility Spectrometry-Driven Shotgun Lipidomics Lintonen, Tuulia P. I.; Baker, Paul R. S.; Suoniemi, Matti Analytical Chemistry, Vol. 86, Issue 19 https://doi.org/10.1021/ac5021744	journal	September 2014
Ion Mobility-Derived Collision Cross Section As an Additional Measure for Lipid Fingerprinting and Identification Paglia, Giuseppe; Angel, Peggi; Williams, Jonathan P. Analytical Chemistry, Vol. 87, Issue 2 https://doi.org/10.1021/ac503715v	journal	December 2014
Mass Spectrometric Collisional Activation and Product Ion Mobility of Human Serum Neutral Lipid Extracts Hankin, Joseph A.; Barkley, Robert M.; Zemski-Berry, Karin Analytical Chemistry, Vol. 88, Issue 12 https://doi.org/10.1021/acs.analchem.6b00292	journal	June 2016
Membrane lipids: where they are and how they behave van Meer, Gerrit; Voelker, Dennis R.; Feigenson, Gerald W. Nature Reviews Molecular Cell Biology, Vol. 9, Issue 2 https://doi.org/10.1038/nrm2330	journal	February 2008
Lipid signalling in disease Wymann, Matthias P.; Schneiter, Roger Nature Reviews Molecular Cell Biology, Vol. 9, Issue 2 https://doi.org/10.1038/nrm2335	journal	February 2008
Cellular lipidomics van Meer, Gerrit The EMBO Journal, Vol. 24, Issue 18 https://doi.org/10.1038/sj.emboj.7600798	journal	September 2005
Quantitative analysis of biological membrane lipids at the low picomole level by nano-electrospray ionization tandem mass spectrometry Brugger, B.; Erben, G.; Sandhoff, R. Proceedings of the National Academy of Sciences, Vol. 94, Issue 6 https://doi.org/10.1073/pnas.94.6.2339	journal	March 1997
A Rapid Method of Total Lipid Extraction and Purification Bligh, E. G.; Dyer, W. J. Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8 https://doi.org/10.1139/o59-099	journal	August 1959
Sphingolipids in High Fat Diet and Obesity-Related Diseases Choi, Songhwa; Snider, Ashley J. Mediators of Inflammation, Vol. 2015 https://doi.org/10.1155/2015/520618	journal	January 2015
From Lipids to Inflammation: New Approaches to Reducing Atherosclerotic Risk Shapiro, Michael D.; Fazio, Sergio Circulation Research, Vol. 118, Issue 4 https://doi.org/10.1161/CIRCRESAHA.115.306471	journal	February 2016
Three-dimensional enhanced lipidomics analysis combining UPLC, differential ion mobility spectrometry, and mass spectrometric separation strategies Baker, Paul R. S.; Armando, Aaron M.; Campbell, J. Larry Journal of Lipid Research, Vol. 55, Issue 11 https://doi.org/10.1194/jlr.D051581	journal	September 2014
A comprehensive classification system for lipids Fahy, Eoin; Subramaniam, Shankar; Brown, H. Alex Journal of Lipid Research, Vol. 46, Issue 5 https://doi.org/10.1194/jlr.E400004-JLR200	journal	February 2005
Lipidomics reveals a remarkable diversity of lipids in human plasma, Quehenberger, Oswald; Armando, Aaron M.; Brown, Alex H. Journal of Lipid Research, Vol. 51, Issue 11 https://doi.org/10.1194/jlr.M009449	journal	July 2010
Shotgun Lipidomics by Sequential Precursor Ion Fragmentation on a Hybrid Quadrupole Time-of-Flight Mass Spectrometer Simons, Brigitte; Kauhanen, Dimple; Sylvänne, Tuulia Metabolites, Vol. 2, Issue 1 https://doi.org/10.3390/metabo2010195	journal	February 2012
T-wave Ion Mobility-mass Spectrometry: Basic Experimental Procedures for Protein Complex Analysis Michaelevski, Izhak; Kirshenbaum, Noam; Sharon, Michal Journal of Visualized Experiments, Issue 41 https://doi.org/10.3791/1985	journal	January 2010

Cited By (2)

Adding a new separation dimension to MS and LC-MS: What is the utility of ion mobility spectrometry? D'Atri, Valentina; Causon, Tim; Hernandez-Alba, Oscar Journal of Separation Science, Vol. 41, Issue 1 https://doi.org/10.1002/jssc.201700919	journal	November 2017
Phosphoinositides in autophagy: current roles and future insights Palamiuc, Lavinia; Ravi, Archna; Emerling, Brooke M. The FEBS Journal, Vol. 287, Issue 2 https://doi.org/10.1111/febs.15127	journal	August 2019

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