Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass
Abstract
The native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants. We determined the chemical composition, cellulose crystallinity and the degree of its polymerization, molecular weight of hemicellulose, and cellulose accessibility of cell walls in order to better understand the fundamental features of why biomass is recalcitrant to conversion without pretreatment. Lastly, the most important is to investigate whether traits and features are stable in the dynamics of field environmental effects over multiple years.
- Authors:
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Joint Institute for Biological Sciences (JIBS); National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1618662
- Alternate Identifier(s):
- OSTI ID: 1339385; OSTI ID: 1340901
- Report Number(s):
- NREL/JA-5100-67649
Journal ID: ISSN 1754-6834; 12; PII: 695
- Grant/Contract Number:
- AC05-00OR22725; AC36-08GO28308
- Resource Type:
- Published Article
- Journal Name:
- Biotechnology for Biofuels
- Additional Journal Information:
- Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- Netherlands
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; Switchgrass; Caffeic acid O-methyltransferase; Biomass recalcitrance; Enzymatic hydrolysis; Lignin; Cellulose accessibility; switchgrass; caffeic acid; O-methyltransferase; biomass recalcitrance; enzymatic hydrolysis; lignin; cellulose accessibility
Citation Formats
Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Gjersing, Erica, Decker, Stephen R., Doeppke, Crissa, Shollenberger, Todd, Tschaplinski, Timothy J., Engle, Nancy L., Sykes, Robert W., Davis, Mark F., Baxter, Holly L., Mazarei, Mitra, Fu, Chunxiang, Dixon, Richard A., Wang, Zeng-Yu, Neal Stewart, Jr., C., and Ragauskas, Arthur J. Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass. Netherlands: N. p., 2017.
Web. doi:10.1186/s13068-016-0695-7.
Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Gjersing, Erica, Decker, Stephen R., Doeppke, Crissa, Shollenberger, Todd, Tschaplinski, Timothy J., Engle, Nancy L., Sykes, Robert W., Davis, Mark F., Baxter, Holly L., Mazarei, Mitra, Fu, Chunxiang, Dixon, Richard A., Wang, Zeng-Yu, Neal Stewart, Jr., C., & Ragauskas, Arthur J. Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass. Netherlands. https://doi.org/10.1186/s13068-016-0695-7
Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Gjersing, Erica, Decker, Stephen R., Doeppke, Crissa, Shollenberger, Todd, Tschaplinski, Timothy J., Engle, Nancy L., Sykes, Robert W., Davis, Mark F., Baxter, Holly L., Mazarei, Mitra, Fu, Chunxiang, Dixon, Richard A., Wang, Zeng-Yu, Neal Stewart, Jr., C., and Ragauskas, Arthur J. Tue .
"Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass". Netherlands. https://doi.org/10.1186/s13068-016-0695-7.
@article{osti_1618662,
title = {Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass},
author = {Li, Mi and Pu, Yunqiao and Yoo, Chang Geun and Gjersing, Erica and Decker, Stephen R. and Doeppke, Crissa and Shollenberger, Todd and Tschaplinski, Timothy J. and Engle, Nancy L. and Sykes, Robert W. and Davis, Mark F. and Baxter, Holly L. and Mazarei, Mitra and Fu, Chunxiang and Dixon, Richard A. and Wang, Zeng-Yu and Neal Stewart, Jr., C. and Ragauskas, Arthur J.},
abstractNote = {The native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants. We determined the chemical composition, cellulose crystallinity and the degree of its polymerization, molecular weight of hemicellulose, and cellulose accessibility of cell walls in order to better understand the fundamental features of why biomass is recalcitrant to conversion without pretreatment. Lastly, the most important is to investigate whether traits and features are stable in the dynamics of field environmental effects over multiple years.},
doi = {10.1186/s13068-016-0695-7},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {Tue Jan 03 00:00:00 EST 2017},
month = {Tue Jan 03 00:00:00 EST 2017}
}
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https://doi.org/10.1186/s13068-016-0695-7
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Cited by: 22 works
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Figures / Tables:
Fig. 1: Chemical composition of field‑grown switchgrass in years 2 and 3. The values (wt% of cell wall residue) reported are the average of 5 biological replicates from each control group and 10 biological replicates from each transgenic group (a). Error bars represent standard errors. Mannan was not detectable. Anmore »
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