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Title: Study of traits and recalcitrance reduction of field-grown COMT down-regulated switchgrass

The native recalcitrance of plants hinders the biomass conversion process using current biorefinery techniques. Down-regulation of the caffeic acid O-methyltransferase (COMT) gene in the lignin biosynthesis pathway of switchgrass reduced the thermochemical and biochemical conversion recalcitrance of biomass. Due to potential environmental influences on lignin biosynthesis and deposition, studying the consequences of physicochemical changes in field-grown plants without pretreatment is essential to evaluate the performance of lignin-altered plants. In this study, we determined the chemical composition, cellulose crystallinity and the degree of its polymerization, molecular weight of hemicellulose, and cellulose accessibility of cell walls in order to better understand the fundamental features of why biomass is recalcitrant to conversion without pretreatment. The most important is to investigate whether traits and features are stable in the dynamics of field environmental effects over multiple years.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [2] ;  [2] ;  [1] ;  [1] ;  [3] ;  [3] ;  [4] ;  [4] ;  [5] ;  [6] ;  [7] ;  [4] ;  [8]
  1. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC), BioSciences Division, and UT-ORNL Joint Institute for Biological Sciences
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States). Biosciences Center
  3. National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of Tennessee, Knoxville, TN (United States). Department of Plant Sciences
  5. The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Forage Improvement Division
  6. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Univ. of North Texas, Denton, TX (United States). BioDiscovery Institute and Department of Biological Sciences
  7. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); The Samuel Roberts Noble Foundation, Ardmore, OK (United States). Forage Improvement Division
  8. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC), BioSciences Division, and UT-ORNL Joint Institute for Biological Sciences; Univ. of Tennessee, Knoxville, TN (United States). Department of Chemical and Biomolecular Engineering & Department of Forestry, Wildlife and Fisheries
Publication Date:
Report Number(s):
NREL/JA-5100-67649
Journal ID: ISSN 1754-6834
Grant/Contract Number:
AC05-00OR22725; AC36-08GO28308
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 10; Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC); Joint Institute for Biological Sciences (JIBS); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES; Switchgrass; Caffeic acid O-methyltransferase; Biomass recalcitrance; Enzymatic hydrolysis; Lignin; Cellulose accessibility; switchgrass; caffeic acid; O-methyltransferase; biomass recalcitrance; enzymatic hydrolysis; lignin; cellulose accessibility
OSTI Identifier:
1339385
Alternate Identifier(s):
OSTI ID: 1340901