¹H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation

Journal Article · Wed Jan 27 04:00:00 UTC 2016 · Metabolomics

DOI: https://doi.org/10.4172/2153-0769.1000165 · OSTI ID:1337278

Xiao, Xiongjie ^[1]; Hu, M. ^[2]; Liu, M. ^[3]; Hu, Jianzhi Z. ^[2]

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1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States); The Chinese Academy of Sciences, Wuhan (People's Republic of China)
2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
3. The Chinese Academy of Sciences, Wuhan (People's Republic of China)

Due to the potential risk of accidental exposure to gamma radiation, it’s critical to identify the biomarkers of radiation exposed creatures. In the present study, NMR based metabolomics combined with multivariate data analysis to evaluate the metabolites changed in the C57BL/6 mouse spleen after 4 days whole body exposure to 3.0 Gy and 7.8 Gy gamma radiations. Principal component analysis (PCA) and orthogonal projection to latent structures analysis (OPLS) are employed for classification and identification potential biomarkers associated with gamma irradiation. Two different strategies for NMR spectral data reduction (i.e., spectral binning and spectral deconvolution) are combined with normalize to constant sum and unit weight before multivariate data analysis, respectively. The combination of spectral deconvolution and normalization to unit weight is the best way for identifying discriminatory metabolites between the irradiation and control groups. Normalized to the constant sum may achieve some pseudo biomarkers. PCA and OPLS results shown that the exposed groups can be well separated from the control group. Leucine, 2-aminobutyrate, valine, lactate, arginine, glutathione, 2-oxoglutarate, creatine, tyrosine, phenylalanine, π-methylhistidine, taurine, myoinositol, glycerol and uracil are significantly elevated while ADP is decreased significantly. As a result, these significantly changed metabolites are associated with multiple metabolic pathways and may be potential biomarkers in the spleen exposed to gamma irradiation.

• Research Organization: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States). Environmental Molecular Sciences Laboratory (EMSL)
• Sponsoring Organization: USDOE
• Grant/Contract Number: AC05-76RL01830
• OSTI ID: 1337278
• Report Number(s): PNNL-SA--119978; 49158; 400412000
• Journal Information: Metabolomics, Journal Name: Metabolomics Journal Issue: 01 Vol. 06; ISSN 2153-0769
• Publisher: OMICS InternationalCopyright Statement
• Country of Publication: United States
• Language: English