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Title: 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation

Abstract

Due to the potential risk of accidental exposure to gamma radiation, it’s critical to identify the biomarkers of radiation exposed creatures. In the present study, NMR based metabolomics combined with multivariate data analysis to evaluate the metabolites changed in the C57BL/6 mouse spleen after 4 days whole body exposure to 3.0 Gy and 7.8 Gy gamma radiations. Principal component analysis (PCA) and orthogonal projection to latent structures analysis (OPLS) are employed for classification and identification potential biomarkers associated with gamma irradiation. Two different strategies for NMR spectral data reduction (i.e., spectral binning and spectral deconvolution) are combined with normalize to constant sum and unit weight before multivariate data analysis, respectively. The combination of spectral deconvolution and normalization to unit weight is the best way for identifying discriminatory metabolites between the irradiation and control groups. Normalized to the constant sum may achieve some pseudo biomarkers. PCA and OPLS results shown that the exposed groups can be well separated from the control group. Leucine, 2-aminobutyrate, valine, lactate, arginine, glutathione, 2-oxoglutarate, creatine, tyrosine, phenylalanine, π-methylhistidine, taurine, myoinositol, glycerol and uracil are significantly elevated while ADP is decreased significantly. As a result, these significantly changed metabolites are associated with multiple metabolic pathways and maymore » be potential biomarkers in the spleen exposed to gamma irradiation.« less

Authors:
 [1];  [2];  [3];  [2]
  1. Pacific Northwest National Lab. (PNNL), Richland, WA (United States); The Chinese Academy of Sciences, Wuhan (People's Republic of China)
  2. Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
  3. The Chinese Academy of Sciences, Wuhan (People's Republic of China)
Publication Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States). Environmental Molecular Sciences Lab. (EMSL)
Sponsoring Org.:
USDOE
OSTI Identifier:
1337278
Report Number(s):
PNNL-SA-119978
Journal ID: ISSN 2153-0769; 49158; 400412000
Grant/Contract Number:  
AC05-76RL01830
Resource Type:
Accepted Manuscript
Journal Name:
Metabolomics
Additional Journal Information:
Journal Volume: 06; Journal Issue: 01; Journal ID: ISSN 2153-0769
Publisher:
OMICS International
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; Environmental Molecular Sciences Laboratory; 1H NMR metabolomics; gamma radiation; spleen; PCA; OPLS; spectral deconvolution

Citation Formats

Xiao, Xiongjie, Hu, M., Liu, M., and Hu, Jianzhi Z. 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation. United States: N. p., 2016. Web. doi:10.4172/2153-0769.1000165.
Xiao, Xiongjie, Hu, M., Liu, M., & Hu, Jianzhi Z. 1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation. United States. doi:10.4172/2153-0769.1000165.
Xiao, Xiongjie, Hu, M., Liu, M., and Hu, Jianzhi Z. Wed . "1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation". United States. doi:10.4172/2153-0769.1000165. https://www.osti.gov/servlets/purl/1337278.
@article{osti_1337278,
title = {1H NMR metabolomics study of spleen from C57BL/6 mice exposed to gamma radiation},
author = {Xiao, Xiongjie and Hu, M. and Liu, M. and Hu, Jianzhi Z.},
abstractNote = {Due to the potential risk of accidental exposure to gamma radiation, it’s critical to identify the biomarkers of radiation exposed creatures. In the present study, NMR based metabolomics combined with multivariate data analysis to evaluate the metabolites changed in the C57BL/6 mouse spleen after 4 days whole body exposure to 3.0 Gy and 7.8 Gy gamma radiations. Principal component analysis (PCA) and orthogonal projection to latent structures analysis (OPLS) are employed for classification and identification potential biomarkers associated with gamma irradiation. Two different strategies for NMR spectral data reduction (i.e., spectral binning and spectral deconvolution) are combined with normalize to constant sum and unit weight before multivariate data analysis, respectively. The combination of spectral deconvolution and normalization to unit weight is the best way for identifying discriminatory metabolites between the irradiation and control groups. Normalized to the constant sum may achieve some pseudo biomarkers. PCA and OPLS results shown that the exposed groups can be well separated from the control group. Leucine, 2-aminobutyrate, valine, lactate, arginine, glutathione, 2-oxoglutarate, creatine, tyrosine, phenylalanine, π-methylhistidine, taurine, myoinositol, glycerol and uracil are significantly elevated while ADP is decreased significantly. As a result, these significantly changed metabolites are associated with multiple metabolic pathways and may be potential biomarkers in the spleen exposed to gamma irradiation.},
doi = {10.4172/2153-0769.1000165},
journal = {Metabolomics},
number = 01,
volume = 06,
place = {United States},
year = {2016},
month = {1}
}

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