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Title: Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice

Journal Article · · Journal of Experimental Botany
DOI: https://doi.org/10.1093/jxb/erw375 · OSTI ID:1332178
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Cornell Univ., Ithaca, NY (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. International Center for Tropical Agriculture, Cali (Columbia)
  4. International Rice Research Institute, Laguna (Philippines)
  5. Bayesic Research, Ithaca, NY (United States)
+ Show Author Affiliations

Rice plants (Oryza sativa) accumulate excess photoassimilates in the form of non-structural carbohydrates (NSCs) in their stems prior to heading that can later be mobilized to supplement photosynthate production during grain-filling. Despite longstanding interest in stem NSC for rice improvement, the dynamics of NSC accumulation, remobilization, and re-accumulation that have genetic potential for optimization have not been systematically investigated. Here we conducted three pilot experiments to lay the groundwork for large-scale diversity studies on rice stem NSC. We assessed the relationship of stem NSC components with 21 agronomic traits in large-scale, tropical yield trials using 33 breeder-nominated lines, established an appropriate experimental design for future genetic studies using a Bayesian framework to sample sub-datasets from highly replicated greenhouse data using 36 genetically diverse genotypes, and used 434 phenotypically divergent rice stem samples to develop two partial least-squares (PLS) models using near-infrared (NIR) spectra for accurate, rapid prediction of rice stem starch, sucrose, and total non-structural carbohydrates. Lastly, we find evidence that stem reserves are most critical for short-duration varieties and suggest that pre-heading stem NSC is worthy of further experimentation for breeding early maturing rice.

Research Organization:
NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))
Sponsoring Organization:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
Grant/Contract Number:
AC36-08GO28308
OSTI ID:
1332178
Report Number(s):
NREL/JA--5100-67277
Journal Information:
Journal of Experimental Botany, Journal Name: Journal of Experimental Botany Journal Issue: 21 Vol. 67; ISSN 0022-0957
Publisher:
Oxford University PressCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (6)

High temperature stress during flowering and grain filling offsets beneficial impact of elevated CO2 on assimilate partitioning and sink-strength in rice journal August 2017
The buffering capacity of stems: genetic architecture of nonstructural carbohydrates in cultivated Asian rice, Oryza sativa journal May 2017
Limitation of Unloading in the Developing Grains Is a Possible Cause Responsible for Low Stem Non-structural Carbohydrate Translocation and Poor Grain Yield Formation in Rice through Verification of Recombinant Inbred Lines journal August 2017
Systems models, phenomics and genomics: three pillars for developing high-yielding photosynthetically efficient crops journal January 2019
Genotypic variation in source and sink traits affects the response of photosynthesis and growth to elevated atmospheric CO 2 journal December 2019
Limitation of Unloading in the Developing Grains Is a Possible Cause Responsible for Low Stem Non-structural Carbohydrate Translocation and Poor Grain Yield Formation in Rice through Verification of Recombinant Inbred Lines journal August 2017

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Oryza sativa
near-infrared spectroscopy
non-structural carbohydrates
partial least-squares
replication
yield potential