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Title: Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice

Abstract

Rice plants (Oryza sativa) accumulate excess photoassimilates in the form of non-structural carbohydrates (NSCs) in their stems prior to heading that can later be mobilized to supplement photosynthate production during grain-filling. Despite longstanding interest in stem NSC for rice improvement, the dynamics of NSC accumulation, remobilization, and re-accumulation that have genetic potential for optimization have not been systematically investigated. Here we conducted three pilot experiments to lay the groundwork for large-scale diversity studies on rice stem NSC. We assessed the relationship of stem NSC components with 21 agronomic traits in large-scale, tropical yield trials using 33 breeder-nominated lines, established an appropriate experimental design for future genetic studies using a Bayesian framework to sample sub-datasets from highly replicated greenhouse data using 36 genetically diverse genotypes, and used 434 phenotypically divergent rice stem samples to develop two partial least-squares (PLS) models using near-infrared (NIR) spectra for accurate, rapid prediction of rice stem starch, sucrose, and total non-structural carbohydrates. Lastly, we find evidence that stem reserves are most critical for short-duration varieties and suggest that pre-heading stem NSC is worthy of further experimentation for breeding early maturing rice.

Authors:
 [1];  [2];  [3];  [4];  [5];  [1]
  1. Cornell Univ., Ithaca, NY (United States)
  2. National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. International Center for Tropical Agriculture, Cali (Columbia)
  4. International Rice Research Institute, Laguna (Philippines)
  5. Bayesic Research, Ithaca, NY (United States)
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1332178
Report Number(s):
NREL/JA-5100-67277
Journal ID: ISSN 0022-0957
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 67; Journal Issue: 21; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; near-infrared spectroscopy; non-structural carbohydrates; Oryza sativa; partial least-squares; replication; yield potential

Citation Formats

Wang, Diane R., Wolfrum, Edward J., Virk, Parminder, Ismail, Abdelbagi, Greenberg, Anthony J., and McCouch, Susan R. Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice. United States: N. p., 2016. Web. doi:10.1093/jxb/erw375.
Wang, Diane R., Wolfrum, Edward J., Virk, Parminder, Ismail, Abdelbagi, Greenberg, Anthony J., & McCouch, Susan R. Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice. United States. doi:https://doi.org/10.1093/jxb/erw375
Wang, Diane R., Wolfrum, Edward J., Virk, Parminder, Ismail, Abdelbagi, Greenberg, Anthony J., and McCouch, Susan R. Wed . "Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice". United States. doi:https://doi.org/10.1093/jxb/erw375. https://www.osti.gov/servlets/purl/1332178.
@article{osti_1332178,
title = {Robust phenotyping strategies for evaluation of stem non-structural carbohydrates (NSC) in rice},
author = {Wang, Diane R. and Wolfrum, Edward J. and Virk, Parminder and Ismail, Abdelbagi and Greenberg, Anthony J. and McCouch, Susan R.},
abstractNote = {Rice plants (Oryza sativa) accumulate excess photoassimilates in the form of non-structural carbohydrates (NSCs) in their stems prior to heading that can later be mobilized to supplement photosynthate production during grain-filling. Despite longstanding interest in stem NSC for rice improvement, the dynamics of NSC accumulation, remobilization, and re-accumulation that have genetic potential for optimization have not been systematically investigated. Here we conducted three pilot experiments to lay the groundwork for large-scale diversity studies on rice stem NSC. We assessed the relationship of stem NSC components with 21 agronomic traits in large-scale, tropical yield trials using 33 breeder-nominated lines, established an appropriate experimental design for future genetic studies using a Bayesian framework to sample sub-datasets from highly replicated greenhouse data using 36 genetically diverse genotypes, and used 434 phenotypically divergent rice stem samples to develop two partial least-squares (PLS) models using near-infrared (NIR) spectra for accurate, rapid prediction of rice stem starch, sucrose, and total non-structural carbohydrates. Lastly, we find evidence that stem reserves are most critical for short-duration varieties and suggest that pre-heading stem NSC is worthy of further experimentation for breeding early maturing rice.},
doi = {10.1093/jxb/erw375},
journal = {Journal of Experimental Botany},
number = 21,
volume = 67,
place = {United States},
year = {2016},
month = {10}
}

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Works referenced in this record:

Yield responses of southern US rice cultivars to CO2 and temperature
journal, April 2004


Non-structural carbohydrate: Analysis by near infrared reflectance spectroscopy and its importance as an indicator of plant growth
journal, October 1993

  • Batten, G. D.; Blakeney, A. B.; McGrath, V. B.
  • Plant and Soil, Vol. 155-156, Issue 1
  • DOI: 10.1007/BF00025029

Contribution of stored pre-anthesis assimilate to grain yield in wheat and barley
journal, December 1977

  • Bidinger, F.; Musgrave, R. B.; Fischer, R. A.
  • Nature, Vol. 270, Issue 5636
  • DOI: 10.1038/270431a0

THE QUANTITATIVE ANALYSIS OF CHLOROPHYLLS a AND b IN PLANT EXTRACTS
journal, April 1963


Molecular regulation of sink–source transition in rice leaf sheaths during the heading period
journal, March 2008


Accumulation of 14C-labelled Carbohydrate Before Flowering and its Subsequent Redistribution and Respiration in the Rice Plant
journal, January 1972

  • CocK, James H.; Yoshida, Shouichi
  • Japanese journal of crop science, Vol. 41, Issue 2
  • DOI: 10.1626/jcs.41.226

Proteomic analysis of a disease-resistance-enhanced lesion mimic mutant spotted leaf 5 in rice
journal, January 2013


Quantitative dynamics of stem water soluble carbohydrates in wheat can be monitored in the field using hyperspectral reflectance
journal, March 2014


Whole Genome Sequencing of Elite Rice Cultivars as a Comprehensive Information Resource for Marker Assisted Selection
journal, April 2015


Pre-anthesis non-structural carbohydrate reserve in the stem enhances the sink strength of inferior spikelets during grain filling of rice
journal, August 2011


Plant-soluble carbohydrate reserves and senescence - key criteria for developing an effective grazing management system for ryegrass-based pastures: a review
journal, January 2001

  • Fulkerson, W. J.; Donaghy, D. J.
  • Australian Journal of Experimental Agriculture, Vol. 41, Issue 2
  • DOI: 10.1071/EA00062

The Effect of the Amount of Nitrogen Fertilizer on Starch Metabolism in Leaf Sheath of Japonica and Indica Rice Varieties during the Heading Period
journal, January 2005

  • Hirano, Tatsuya; Saito, Yoshihisa; Ushimaru, Hiroaki
  • Plant Production Science, Vol. 8, Issue 2
  • DOI: 10.1626/pps.8.122

The map-based sequence of the rice genome
journal, August 2005


The International Oryza Map Alignment Project: development of a genus-wide comparative genomics platform to help solve the 9 billion-people question
journal, May 2013

  • Jacquemin, Julie; Bhatia, Dharminder; Singh, Kuldeep
  • Current Opinion in Plant Biology, Vol. 16, Issue 2
  • DOI: 10.1016/j.pbi.2013.02.014

Identification and Functional Analysis of a Locus for Improvement of Lodging Resistance in Rice
journal, January 2004


Locus prl5 improves lodging resistance of rice by delaying senescence and increasing carbohydrate reaccumulation
journal, February 2006


Improvement of lodging resistance with QTLs for stem diameter in rice (Oryza sativa L.)
journal, June 2008

  • Kashiwagi, Takayuki; Togawa, Eiji; Hirotsu, Naoki
  • Theoretical and Applied Genetics, Vol. 117, Issue 5
  • DOI: 10.1007/s00122-008-0816-1

Relationship between grain filling duration and leaf senescence of temperate rice under high temperature
journal, June 2011


CGtag: complete genomics toolkit and annotation in a cloud-based Galaxy
journal, January 2014

  • Hiltemann, Saskia; Mei, Hailiang; de Hollander, Mattias
  • GigaScience, Vol. 3, Issue 1
  • DOI: 10.1186/2047-217X-3-1

Open access resources for genome-wide association mapping in rice
journal, February 2016

  • McCouch, Susan R.; Wright, Mark H.; Tung, Chih-Wei
  • Nature Communications, Vol. 7, Issue 1
  • DOI: 10.1038/ncomms10532

Temporal and Spatial Regulation of Sucrose Accumulation in the Sugarcane Stem
journal, January 1995


Growth dynamics and genotypic variation in tropical, field‐grown paddy rice (Oryza sativa L.) in response to increasing carbon dioxide and temperature
journal, August 1998


Plot size matters: interference from intergenotypic competition in plant phenotyping studies
journal, January 2014

  • Rebetzke, Greg J.; Fischer, Ralph (Tony) A.; van Herwaarden, Anthony F.
  • Functional Plant Biology, Vol. 41, Issue 2
  • DOI: 10.1071/FP13177

Genotypic variation in water-soluble carbohydrate accumulation in wheat
journal, January 2006

  • Ruuska, Sari A.; Rebetzke, Greg J.; van Herwaarden, Anthony F.
  • Functional Plant Biology, Vol. 33, Issue 9
  • DOI: 10.1071/FP06062

Seasonal Dynamics of Nonstructural Carbohydrate Partitioning in 15 Diverse Rice Genotypes
journal, May 2001


Determination of blood glucose using an oxidase-peroxidase system with a non-carcinogenic chromogen
journal, March 1969


Sugarcane for bioenergy production: an assessment of yield and regulation of sucrose content
journal, April 2010


Evidence for divergence of response in Indica , Japonica , and wild rice to high CO 2 × temperature interaction
journal, April 2016

  • Wang, Diane R.; Bunce, James A.; Tomecek, Martha B.
  • Global Change Biology, Vol. 22, Issue 7
  • DOI: 10.1111/gcb.13279

Remobilization of Carbon Reserves Is Improved by Controlled Soil-Drying during Grain Filling of Wheat
journal, November 2000


    Works referencing / citing this record: