Development of an optical Zn2+ probe based on a single fluorescent protein
Abstract
Various fluorescent probes have been developed to reveal the biological functions of intracellular labile Zn2+. Here we present Green Zinc Probe (GZnP), a novel genetically encoded Zn2+ sensor design based on a single fluorescent protein (single-FP). The GZnP sensor is generated by attaching two zinc fingers (ZF) of the transcription factor Zap1 (ZF1 and ZF2) to the two ends of a circularly permuted green fluorescent protein (cpGFP). Formation of ZF folds induces interaction between the two ZFs, which induces a change in the cpGFP conformation, leading to an increase in fluorescence. A small sensor library is created to include mutations in the ZFs, cpGFP and linkers between ZF and cpGFP to improve signal stability, sensor brightness and dynamic range based on rational protein engineering and computational design by Rosetta. Using a cell-based library screen, we identify sensor GZnP1 which demonstrates a stable maximum signal, decent brightness (QY = 0.42 at apo state), as well as specific and sensitive response to Zn2+ in HeLa cells (Fmax/Fmin = 2.6, Kd = 58 pM, pH 7.4). The subcellular localizing sensors mito-GZnP1 (in mitochondria matrix) and Lck-GZnP1 (on plasma membrane) display sensitivity to Zn2+ (Fmax/Fmin = 2.2). In conclusion, this sensor design provides freedommore »
- Authors:
-
- Univ. of Colorado, Boulder, CO (United States)
- National Renewable Energy Lab. (NREL), Golden, CO (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office
- OSTI Identifier:
- 1330942
- Report Number(s):
- NREL/JA-2700-66894
Journal ID: ISSN 1554-8929
- Grant/Contract Number:
- AC36-08GO28308
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Chemical Biology
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 10; Journal ID: ISSN 1554-8929
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; zinc; genetically encoded sensor; single fluorescent protein; Rosetta
Citation Formats
Qin, Yan, Sammond, Deanne W., Braselmann, Esther, Carpenter, Margaret C., and Palmer, Amy E. Development of an optical Zn2+ probe based on a single fluorescent protein. United States: N. p., 2016.
Web. doi:10.1021/acschembio.6b00442.
Qin, Yan, Sammond, Deanne W., Braselmann, Esther, Carpenter, Margaret C., & Palmer, Amy E. Development of an optical Zn2+ probe based on a single fluorescent protein. United States. https://doi.org/10.1021/acschembio.6b00442
Qin, Yan, Sammond, Deanne W., Braselmann, Esther, Carpenter, Margaret C., and Palmer, Amy E. Thu .
"Development of an optical Zn2+ probe based on a single fluorescent protein". United States. https://doi.org/10.1021/acschembio.6b00442. https://www.osti.gov/servlets/purl/1330942.
@article{osti_1330942,
title = {Development of an optical Zn2+ probe based on a single fluorescent protein},
author = {Qin, Yan and Sammond, Deanne W. and Braselmann, Esther and Carpenter, Margaret C. and Palmer, Amy E.},
abstractNote = {Various fluorescent probes have been developed to reveal the biological functions of intracellular labile Zn2+. Here we present Green Zinc Probe (GZnP), a novel genetically encoded Zn2+ sensor design based on a single fluorescent protein (single-FP). The GZnP sensor is generated by attaching two zinc fingers (ZF) of the transcription factor Zap1 (ZF1 and ZF2) to the two ends of a circularly permuted green fluorescent protein (cpGFP). Formation of ZF folds induces interaction between the two ZFs, which induces a change in the cpGFP conformation, leading to an increase in fluorescence. A small sensor library is created to include mutations in the ZFs, cpGFP and linkers between ZF and cpGFP to improve signal stability, sensor brightness and dynamic range based on rational protein engineering and computational design by Rosetta. Using a cell-based library screen, we identify sensor GZnP1 which demonstrates a stable maximum signal, decent brightness (QY = 0.42 at apo state), as well as specific and sensitive response to Zn2+ in HeLa cells (Fmax/Fmin = 2.6, Kd = 58 pM, pH 7.4). The subcellular localizing sensors mito-GZnP1 (in mitochondria matrix) and Lck-GZnP1 (on plasma membrane) display sensitivity to Zn2+ (Fmax/Fmin = 2.2). In conclusion, this sensor design provides freedom to be used in combination with other optical indicators and optogenetic tools for simultaneous imaging and advancing our understanding of cellular Zn2+ function.},
doi = {10.1021/acschembio.6b00442},
journal = {ACS Chemical Biology},
number = 10,
volume = 11,
place = {United States},
year = {Thu Jul 28 00:00:00 EDT 2016},
month = {Thu Jul 28 00:00:00 EDT 2016}
}
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