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Title: Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype

Abstract

Human NADPH-cytochrome P450 oxidoreductase (POR) gene mutations are associated with severe skeletal deformities and disordered steroidogenesis. The human POR mutation A287P presents with disordered sexual development and skeletal malformations. Difficult recombinant expression and purification of this POR mutant suggested that the protein was less stable than WT. The activities of cytochrome P450 17A1, 19A1, and 21A2, critical in steroidogenesis, were similar using our purified, full-length, unmodified A287P or WT POR, as were those of several xenobiotic-metabolizing cytochromes P450, indicating that the A287P protein is functionally competent in vitro, despite its functionally deficient phenotypic behavior in vivo. Differential scanning calorimetry and limited trypsinolysis studies revealed a relatively unstable A287P compared with WT protein, leading to the hypothesis that the syndrome observed in vivo results from altered POR protein stability. The crystal structures of the soluble domains of WT and A287P reveal only subtle differences between them, but these differences are consistent with the differential scanning calorimetry results as well as the differential susceptibility of A287P and WT observed with trypsinolysis. The relative in vivo stabilities of WT and A287P proteins were also examined in an osteoblast cell line by treatment with cycloheximide, a protein synthesis inhibitor, showing that the level ofmore » A287P protein post-inhibition is lower than WT and suggesting that A287P may be degraded at a higher rate. Overall, current studies demonstrate that, unlike previously described mutations, A287P causes POR deficiency disorder due to conformational instability leading to proteolytic susceptibility in vivo, rather than through an inherent flavin-binding defect.« less

Authors:
 [1];  [1];  [2];  [2];  [3];  [3];  [4];  [1];  [5];  [6];  [1];  [1];  [1]
+ Show Author Affiliations
  1. Univ. of Texas Health Science Center, San Antonio, TX (United States)
  2. Medical College of Wisconsin, Milwaukee, WI (United States)
  3. Univ. Nova de Lisboa (Portugal)
  4. Univ. of Michigan, Ann Arbor, MI (United States)
  5. State Univ. of New York Upstate Medical Univ., Syracuse, NY (United States)
  6. Charles Univ. in Prague (Czech Republic); General Univ. Hospital (Czech Republic)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
Sponsoring Org.:
National Institutes of Health (NIH); Fundação para a Ciência e a Tecnologia (FCT)
OSTI Identifier:
1328798
Grant/Contract Number:  
GM081568; GM097031; PTDC/SAU-GMG/71911/2006; UID/BIM/00009/ 2013; GM086596; GM086893; UNCE 204011; PRVOUK P24/LF1/3; RVO-VFN 64165/2012
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Biological Chemistry
Additional Journal Information:
Journal Volume: 291; Journal Issue: 39; Journal ID: ISSN 0021-9258
Publisher:
American Society for Biochemistry and Molecular Biology
Country of Publication:
United States
Language:
ENGLISH
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; cytochrome P450; flavoprotein; membrane protein; protein crystallization; protein stability; reductase; POR deficiency; cytochrome P450 oxidoreductase; diflavin oxidoreductase

Citation Formats

McCammon, Karen M., Panda, Satya P., Xia, Chuanwu, Kim, Jung-Ja P., Moutinho, Daniela, Kranendonk, Michel, Auchus, Richard J., Lafer, Eileen M., Ghosh, Debashis, Martasek, Pavel, Kar, Rekha, Masters, Bettie Sue, and Roman, Linda J. Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype. United States: N. p., 2016. Web. doi:10.1074/jbc.M116.716019.
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McCammon, Karen M., Panda, Satya P., Xia, Chuanwu, Kim, Jung-Ja P., Moutinho, Daniela, Kranendonk, Michel, Auchus, Richard J., Lafer, Eileen M., Ghosh, Debashis, Martasek, Pavel, Kar, Rekha, Masters, Bettie Sue, & Roman, Linda J. Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype. United States. https://doi.org/10.1074/jbc.M116.716019
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McCammon, Karen M., Panda, Satya P., Xia, Chuanwu, Kim, Jung-Ja P., Moutinho, Daniela, Kranendonk, Michel, Auchus, Richard J., Lafer, Eileen M., Ghosh, Debashis, Martasek, Pavel, Kar, Rekha, Masters, Bettie Sue, and Roman, Linda J. Fri . "Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype". United States. https://doi.org/10.1074/jbc.M116.716019. https://www.osti.gov/servlets/purl/1328798.
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@article{osti_1328798,
title = {Instability of the Human Cytochrome P450 Reductase A287P Variant Is the Major Contributor to Its Antley-Bixler Syndrome-like Phenotype},
author = {McCammon, Karen M. and Panda, Satya P. and Xia, Chuanwu and Kim, Jung-Ja P. and Moutinho, Daniela and Kranendonk, Michel and Auchus, Richard J. and Lafer, Eileen M. and Ghosh, Debashis and Martasek, Pavel and Kar, Rekha and Masters, Bettie Sue and Roman, Linda J.},
abstractNote = {Human NADPH-cytochrome P450 oxidoreductase (POR) gene mutations are associated with severe skeletal deformities and disordered steroidogenesis. The human POR mutation A287P presents with disordered sexual development and skeletal malformations. Difficult recombinant expression and purification of this POR mutant suggested that the protein was less stable than WT. The activities of cytochrome P450 17A1, 19A1, and 21A2, critical in steroidogenesis, were similar using our purified, full-length, unmodified A287P or WT POR, as were those of several xenobiotic-metabolizing cytochromes P450, indicating that the A287P protein is functionally competent in vitro, despite its functionally deficient phenotypic behavior in vivo. Differential scanning calorimetry and limited trypsinolysis studies revealed a relatively unstable A287P compared with WT protein, leading to the hypothesis that the syndrome observed in vivo results from altered POR protein stability. The crystal structures of the soluble domains of WT and A287P reveal only subtle differences between them, but these differences are consistent with the differential scanning calorimetry results as well as the differential susceptibility of A287P and WT observed with trypsinolysis. The relative in vivo stabilities of WT and A287P proteins were also examined in an osteoblast cell line by treatment with cycloheximide, a protein synthesis inhibitor, showing that the level of A287P protein post-inhibition is lower than WT and suggesting that A287P may be degraded at a higher rate. Overall, current studies demonstrate that, unlike previously described mutations, A287P causes POR deficiency disorder due to conformational instability leading to proteolytic susceptibility in vivo, rather than through an inherent flavin-binding defect.},
doi = {10.1074/jbc.M116.716019},
journal = {Journal of Biological Chemistry},
number = 39,
volume = 291,
place = {United States},
year = {Fri Aug 05 00:00:00 EDT 2016},
month = {Fri Aug 05 00:00:00 EDT 2016}
}
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https://doi.org/10.1074/jbc.M116.716019
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