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Title: Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process

Abstract

With this study, to understand the structural changes of lignin after soda-AQ and kraft pretreatment, milled straw lignin, black liquor lignin and residual lignin extracted from wheat straw were characterized by FT-IR, UV, GPC and NMR. The results showed that the main lignin linkages were β-aryl ether substructures (β-O-4'), followed by phenylcoumaran (β-5') and resinol (β-β') substructures, while minor content of spirodienone (β-1'), dibenzodioxocin (5-5') and α,β-diaryl ether linkages were detected as well. After pretreatment, most lignin inter-units and lignin-carbohydrate complex (LCC) linkages were degraded and dissolved in black liquor, with minor amount left in residual pretreated biomass. In addition, through quantitative 13C and 2D-HSQC NMR spectral analysis, lignin and LCC were found to be more degraded after kraft pretreatment than soda-AQ pretreatment. Furthermore, the subsequent enzymatic hydrolysis results showed that more cellulose in wheat straw was converted to glucose after kraft pretreatment, indicating that LCC linkages were important in the enzymatic hydrolysis process.

Authors:
 [1];  [2];  [2];  [3];  [3];  [3];  [3];  [4];  [5]
  1. Hubei Univ. of Technology, Wuhan (China); Georgia Inst. of Technology, Atlanta, GA (United States)
  2. Hubei Univ. of Technology, Wuhan (China)
  3. Georgia Inst. of Technology, Atlanta, GA (United States)
  4. Georgia Inst. of Technology, Atlanta, GA (United States); Univ. of Tennessee, Knoxville, TN (United States)
  5. Hubei Univ. of Technology, Wuhan (China); South China Univ. of Technology, Guangzhou (China)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1327692
Grant/Contract Number:  
AC05-00OR22725
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 207; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; soda-AQ pretreatment; kraft pretreatment; lignin; HSQC; enzymatic hydrolysis

Citation Formats

Yang, Haitao, Xie, Yimin, Zheng, Xing, Pu, Yunqiao, Huang, Fang, Meng, Xianzhi, Wu, Weibing, Ragauskas, Arthur, and Yao, Lan. Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process. United States: N. p., 2016. Web. doi:10.1016/j.biortech.2016.01.123.
Yang, Haitao, Xie, Yimin, Zheng, Xing, Pu, Yunqiao, Huang, Fang, Meng, Xianzhi, Wu, Weibing, Ragauskas, Arthur, & Yao, Lan. Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process. United States. https://doi.org/10.1016/j.biortech.2016.01.123
Yang, Haitao, Xie, Yimin, Zheng, Xing, Pu, Yunqiao, Huang, Fang, Meng, Xianzhi, Wu, Weibing, Ragauskas, Arthur, and Yao, Lan. Thu . "Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process". United States. https://doi.org/10.1016/j.biortech.2016.01.123. https://www.osti.gov/servlets/purl/1327692.
@article{osti_1327692,
title = {Comparative study of lignin characteristics from wheat straw obtained by soda-AQ and kraft pretreatment and effect on the following enzymatic hydrolysis process},
author = {Yang, Haitao and Xie, Yimin and Zheng, Xing and Pu, Yunqiao and Huang, Fang and Meng, Xianzhi and Wu, Weibing and Ragauskas, Arthur and Yao, Lan},
abstractNote = {With this study, to understand the structural changes of lignin after soda-AQ and kraft pretreatment, milled straw lignin, black liquor lignin and residual lignin extracted from wheat straw were characterized by FT-IR, UV, GPC and NMR. The results showed that the main lignin linkages were β-aryl ether substructures (β-O-4'), followed by phenylcoumaran (β-5') and resinol (β-β') substructures, while minor content of spirodienone (β-1'), dibenzodioxocin (5-5') and α,β-diaryl ether linkages were detected as well. After pretreatment, most lignin inter-units and lignin-carbohydrate complex (LCC) linkages were degraded and dissolved in black liquor, with minor amount left in residual pretreated biomass. In addition, through quantitative 13C and 2D-HSQC NMR spectral analysis, lignin and LCC were found to be more degraded after kraft pretreatment than soda-AQ pretreatment. Furthermore, the subsequent enzymatic hydrolysis results showed that more cellulose in wheat straw was converted to glucose after kraft pretreatment, indicating that LCC linkages were important in the enzymatic hydrolysis process.},
doi = {10.1016/j.biortech.2016.01.123},
journal = {Bioresource Technology},
number = C,
volume = 207,
place = {United States},
year = {2016},
month = {2}
}

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  • Parnthong, Jatuporn; Kungsanant, Suratsawadee; Chavadej, Sumaeth
  • Applied Biochemistry and Biotechnology, Vol. 186, Issue 4
  • DOI: 10.1007/s12010-018-2783-6

Degradation of Vanillin During Lignin Valorization Under Alkaline Oxidation
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Characterization and Integrated Process of Pretreatment and Enzymatic Hydrolysis of Corn Straw
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