Selective synthesis of human milk fat-style structured triglycerides from microalgal oil in a microfluidic reactor packed with immobilized lipase

Wang, Jun; Liu, Xi; Wang, Xu -Dong; Dong, Tao; Zhao, Xing -Yu; Zhu, Dan; Mei, Yi -Yuan; Wu, Guo -Hua

doi:10.1016/j.biortech.2016.08.023

Title: Selective synthesis of human milk fat-style structured triglycerides from microalgal oil in a microfluidic reactor packed with immobilized lipase

Journal Article · Thu Aug 18 04:00:00 UTC 2016 · Bioresource Technology

DOI: https://doi.org/10.1016/j.biortech.2016.08.023 · OSTI ID:1326324

Wang, Jun ^[1]; Liu, Xi ^[2]; Wang, Xu -Dong ^[2]; Dong, Tao ^[3]; Zhao, Xing -Yu ^[2]; Zhu, Dan ^[2]; Mei, Yi -Yuan ^[2]; Wu, Guo -Hua ^[1]

Jiangsu Univ. of Science and Technology, Zhenjiang (People's Republic of China); Chinese Academy of Agricultural Sciences, Zhenjiang (People's Republic of China)
Jiangsu Univ. of Science and Technology, Zhenjiang (People's Republic of China)
National Renewable Energy Lab. (NREL), Golden, CO (United States)

Human milk fat-style structured triacylglycerols were produced from microalgal oil in a continuous microfluidic reactor packed with immobilized lipase for the first time. A remarkably high conversion efficiency was demonstrated in the microreactor with reaction time being reduced by 8 times, Michaelis constant decreased 10 times, the lipase reuse times increased 2.25-fold compared to those in a batch reactor. In addition, the content of palmitic acid at sn-2 position (89.0%) and polyunsaturated fatty acids at sn-1, 3 positions (81.3%) are slightly improved compared to the product in a batch reactor. The increase of melting points (1.7 °C) and decrease of crystallizing point (3 °C) implied higher quality product was produced using the microfluidic technology. The main cost can be reduced from 212.3 to 14.6 per batch with the microreactor. Altogether, the microfluidic bioconversion technology is promising for modified functional lipids production allowing for cost-effective approach to produce high-value microalgal coproducts.

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Research Organization:: NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))

Sponsoring Organization:: Jiangsu Province; Jiangsu University of Science and Technology; Modern Agro-industry Technology Research System of China; USDOE

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1326324

Report Number(s):: NREL/JA-5100--67110

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Journal Issue: C Vol. 220; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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Title: Selective synthesis of human milk fat-style structured triglycerides from microalgal oil in a microfluidic reactor packed with immobilized lipase

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