The unusual and dynamic character of PX-DNA

Niu, Dong; Jiang, Hualin; Sha, Ruojie; Canary, James W.; Seeman, Nadrian C.

doi:10.1093/nar/gkv739

Title: The unusual and dynamic character of PX-DNA

Journal Article · Wed Jul 15 04:00:00 UTC 2015 · Nucleic Acids Research

DOI: https://doi.org/10.1093/nar/gkv739 · OSTI ID:1326213

Niu, Dong ^[1]; Jiang, Hualin ^[2]; Sha, Ruojie ^[2]; Canary, James W. ^[2]; Seeman, Nadrian C. ^[2]

New York Univ. (NYU), NY (United States). Dept. of Chemistry; New York University
New York Univ. (NYU), NY (United States). Dept. of Chemistry

PX-DNA is a four-stranded DNA structure that has been implicated in the recognition of homology, either continuously, or in an every-other-half-turn fashion. Some of the structural features of the molecule have been noted previously, but the structure requires further characterization. Here, we report atomic force microscopic characterization of PX molecules that contain periodically placed biotin groups, enabling the molecule to be labeled by streptavidin molecules at these sites. In comparison with conventional double stranded DNA and with antiparallel DNA double crossover molecules, it is clear that PX-DNA is a more dynamic structure. Moreover, the spacing between the nucleotide pairs along the helix axis is shorter, suggesting a mixed B/A structure. Circular dichroism spectroscopy indicates unusual features in the PX molecule that are absent in both the molecules to which it is compared.

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Research Organization:: New York Univ. (NYU), NY (United States)

Sponsoring Organization:: USDOE Office of Science (SC); National Inst. of Health (NIH); National Science Foundation (NSF); US Army Research Office (ARO); US Department of the Navy, Office of Naval Research (ONR); Gordon and Betty Moore Foundation

Grant/Contract Number:: SC0007991

OSTI ID:: 1326213

Journal Information:: Nucleic Acids Research, Journal Name: Nucleic Acids Research Journal Issue: 15 Vol. 43; ISSN 0305-1048

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: United States

Language:: English

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Recombination-Independent Recognition of DNA Homology for Repeat-Induced Point Mutation (RIP) Is Modulated by the Underlying Nucleotide Sequence Gladyshev, Eugene; Kleckner, Nancy PLOS Genetics, Vol. 12, Issue 5 https://doi.org/10.1371/journal.pgen.1006015	journal	May 2016

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