Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes
Abstract
Understanding and improving the productivity and robustness of plant photosynthesis requires high-throughput phenotyping under environmental conditions that are relevant to the field. Here we demonstrate the dynamic environmental photosynthesis imager (DEPI), an experimental platform for integrated, continuous, and high-throughput measurements of photosynthetic parameters during plant growth under reproducible yet dynamic environmental conditions. Using parallel imagers obviates the need to move plants or sensors, reducing artifacts and allowing simultaneous measurement on large numbers of plants. As a result, DEPI can reveal phenotypes that are not evident under standard laboratory conditions but emerge under progressively more dynamic illumination. We show examples in mutants of Arabidopsis of such “emergent phenotypes” that are highly transient and heterogeneous, appearing in different leaves under different conditions and depending in complex ways on both environmental conditions and plant developmental age. Finally, these emergent phenotypes appear to be caused by a range of phenomena, suggesting that such previously unseen processes are critical for plant responses to dynamic environments.
- Authors:
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1325328
- Alternate Identifier(s):
- OSTI ID: 1437168
- Grant/Contract Number:
- FG02-91ER20021
- Resource Type:
- Published Article
- Journal Name:
- Cell Systems
- Additional Journal Information:
- Journal Name: Cell Systems Journal Volume: 2 Journal Issue: 6; Journal ID: ISSN 2405-4712
- Publisher:
- Elsevier
- Country of Publication:
- Niger
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; abiotic stress; chlorophyll fluorescence; biotic stress; imaging; phenometrics; phenomics; photosynthesis
Citation Formats
Cruz, Jeffrey A., Savage, Linda J., Zegarac, Robert, Hall, Christopher C., Satoh-Cruz, Mio, Davis, Geoffry A., Kovac, William Kent, Chen, Jin, and Kramer, David M. Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes. Niger: N. p., 2016.
Web. doi:10.1016/j.cels.2016.06.001.
Cruz, Jeffrey A., Savage, Linda J., Zegarac, Robert, Hall, Christopher C., Satoh-Cruz, Mio, Davis, Geoffry A., Kovac, William Kent, Chen, Jin, & Kramer, David M. Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes. Niger. https://doi.org/10.1016/j.cels.2016.06.001
Cruz, Jeffrey A., Savage, Linda J., Zegarac, Robert, Hall, Christopher C., Satoh-Cruz, Mio, Davis, Geoffry A., Kovac, William Kent, Chen, Jin, and Kramer, David M. Wed .
"Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes". Niger. https://doi.org/10.1016/j.cels.2016.06.001.
@article{osti_1325328,
title = {Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes},
author = {Cruz, Jeffrey A. and Savage, Linda J. and Zegarac, Robert and Hall, Christopher C. and Satoh-Cruz, Mio and Davis, Geoffry A. and Kovac, William Kent and Chen, Jin and Kramer, David M.},
abstractNote = {Understanding and improving the productivity and robustness of plant photosynthesis requires high-throughput phenotyping under environmental conditions that are relevant to the field. Here we demonstrate the dynamic environmental photosynthesis imager (DEPI), an experimental platform for integrated, continuous, and high-throughput measurements of photosynthetic parameters during plant growth under reproducible yet dynamic environmental conditions. Using parallel imagers obviates the need to move plants or sensors, reducing artifacts and allowing simultaneous measurement on large numbers of plants. As a result, DEPI can reveal phenotypes that are not evident under standard laboratory conditions but emerge under progressively more dynamic illumination. We show examples in mutants of Arabidopsis of such “emergent phenotypes” that are highly transient and heterogeneous, appearing in different leaves under different conditions and depending in complex ways on both environmental conditions and plant developmental age. Finally, these emergent phenotypes appear to be caused by a range of phenomena, suggesting that such previously unseen processes are critical for plant responses to dynamic environments.},
doi = {10.1016/j.cels.2016.06.001},
journal = {Cell Systems},
number = 6,
volume = 2,
place = {Niger},
year = {Wed Jun 01 00:00:00 EDT 2016},
month = {Wed Jun 01 00:00:00 EDT 2016}
}
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https://doi.org/10.1016/j.cels.2016.06.001
https://doi.org/10.1016/j.cels.2016.06.001
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Cited by: 75 works
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