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Title: Dynamic Environmental Photosynthetic Imaging Reveals Emergent Phenotypes

Understanding and improving the productivity and robustness of plant photosynthesis requires high-throughput phenotyping under environmental conditions that are relevant to the field. Here we demonstrate the dynamic environmental photosynthesis imager (DEPI), an experimental platform for integrated, continuous, and high-throughput measurements of photosynthetic parameters during plant growth under reproducible yet dynamic environmental conditions. Using parallel imagers obviates the need to move plants or sensors, reducing artifacts and allowing simultaneous measurement on large numbers of plants. As a result, DEPI can reveal phenotypes that are not evident under standard laboratory conditions but emerge under progressively more dynamic illumination. We show examples in mutants of Arabidopsis of such “emergent phenotypes” that are highly transient and heterogeneous, appearing in different leaves under different conditions and depending in complex ways on both environmental conditions and plant developmental age. Finally, these emergent phenotypes appear to be caused by a range of phenomena, suggesting that such previously unseen processes are critical for plant responses to dynamic environments.
Authors:
 [1] ;  [2] ;  [2] ;  [3] ;  [2] ;  [4] ;  [3] ;  [5] ;  [6]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Michigan State Univ., East Lansing, MI (United States). Biochemistry and Molecular Biology
  2. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab
  3. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab; Michigan State Univ., East Lansing, MI (United States). Plant Biology
  4. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Michigan State Univ., East Lansing, MI (United States). Cell and Molecular Biology
  5. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Michigan State Univ., East Lansing, MI (United States). Computer Science and Engineerin
  6. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Lab.; Michigan State Univ., East Lansing, MI (United States). Biochemistry and Molecular Biology; Michigan State Univ., East Lansing, MI (United States). Plant Biology
Publication Date:
Grant/Contract Number:
FG02-91ER20021
Type:
Published Article
Journal Name:
Cell Systems
Additional Journal Information:
Journal Volume: 2; Journal Issue: 6; Journal ID: ISSN 2405-4712
Research Org:
Michigan State Univ., East Lansing, MI (United States)
Sponsoring Org:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; abiotic stress; chlorophyll fluorescence; biotic stress; imaging; phenometrics; phenomics; photosynthesis
OSTI Identifier:
1325328
Alternate Identifier(s):
OSTI ID: 1437168