Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning

Welsher, Kevin; McManus, Simon A.; Hsia, Chih -Hao; Yin, Shuhui; Yang, Haw

doi:10.1021/ja511297d

Title: Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning

Abstract

The seemingly inevitable protein corona appears to be an insurmountable obstacle to wider application of functional nanomaterials in biotechnology. The accumulation of serum proteins can block targeting functionalities and alter the in vivo fate of these nanomaterials. Here we demonstrate a method to generate non-stick, robustly passivated functional nanoparticles (NPs) using a tailored silica coating. We apply agarose gel electrophoresis to sensitively evaluate protein binding to NPs with different surface chemistry. Using gel banding and retardation as a read-out for protein adsorption, we optimize the surface chemistry to yield a mixed charge surface which displays remarkable binding resistance to a wide range of serum proteins and nucleic acids. Here, the hard silica shell also protects the functional NP core in harsh environments (down to pH 1) while still showing the ability to be targeted for cellular uptake with little or no non-specific binding.

Authors:

Welsher, Kevin ^[1]; McManus, Simon A. ^[1]; Hsia, Chih -Hao ^[1]; Yin, Shuhui ^[1]; Yang, Haw ^[1]

Princeton Univ., Princeton, NJ (United States)

Publication Date:: Mon Jan 05 00:00:00 EST 2015

Research Org.:: Princeton Univ., NJ (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1324661

Grant/Contract Number:: SC0006838

Resource Type:: Accepted Manuscript

Journal Name:: Journal of the American Chemical Society

Additional Journal Information:: Journal Volume: 137; Journal Issue: 2; Journal ID: ISSN 0002-7863

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; 36 MATERIALS SCIENCE; nanomaterials; biotechnology; protein

Citation Formats


                    Welsher, Kevin, McManus, Simon A., Hsia, Chih -Hao, Yin, Shuhui, and Yang, Haw. Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning.  United States: N. p., 2015. 
Web.  doi:10.1021/ja511297d.

Copy to clipboard


                    Welsher, Kevin, McManus, Simon A., Hsia, Chih -Hao, Yin, Shuhui, & Yang, Haw. Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning.  United States.  https://doi.org/10.1021/ja511297d

Copy to clipboard


                    Welsher, Kevin, McManus, Simon A., Hsia, Chih -Hao, Yin, Shuhui, and Yang, Haw. Mon .  
"Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning".  United States.  https://doi.org/10.1021/ja511297d.  https://www.osti.gov/servlets/purl/1324661.

Copy to clipboard


                    
@article{osti_1324661,

  title        = {Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning},

  author       = {Welsher, Kevin and McManus, Simon A. and Hsia, Chih -Hao and Yin, Shuhui and Yang, Haw},

  abstractNote = {The seemingly inevitable protein corona appears to be an insurmountable obstacle to wider application of functional nanomaterials in biotechnology. The accumulation of serum proteins can block targeting functionalities and alter the in vivo fate of these nanomaterials. Here we demonstrate a method to generate non-stick, robustly passivated functional nanoparticles (NPs) using a tailored silica coating. We apply agarose gel electrophoresis to sensitively evaluate protein binding to NPs with different surface chemistry. Using gel banding and retardation as a read-out for protein adsorption, we optimize the surface chemistry to yield a mixed charge surface which displays remarkable binding resistance to a wide range of serum proteins and nucleic acids. Here, the hard silica shell also protects the functional NP core in harsh environments (down to pH 1) while still showing the ability to be targeted for cellular uptake with little or no non-specific binding.},

  doi          = {10.1021/ja511297d},

  journal      = {Journal of the American Chemical Society},

  number       = 2,

  volume       = 137,

  place        = {United States},

  year         = {Mon Jan 05 00:00:00 EST 2015},

  month        = {Mon Jan 05 00:00:00 EST 2015}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

https://doi.org/10.1021/ja511297d

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 24 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

Understanding and controlling the interaction of nanomaterials with proteins in a physiological environment
journal, January 2012

Walkey, Carl D.; Chan, Warren C. W.
Chem. Soc. Rev., Vol. 41, Issue 7
DOI: 10.1039/C1CS15233E

Biomolecular coronas provide the biological identity of nanosized materials
journal, December 2012

Monopoli, Marco P.; Åberg, Christoffer; Salvati, Anna
Nature Nanotechnology, Vol. 7, Issue 12
DOI: 10.1038/nnano.2012.207

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts
journal, September 2008

Lundqvist, M.; Stigler, J.; Elia, G.
Proceedings of the National Academy of Sciences, Vol. 105, Issue 38
DOI: 10.1073/pnas.0805135105

Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
journal, January 2007

Cedervall, T.; Lynch, I.; Lindman, S.
Proceedings of the National Academy of Sciences, Vol. 104, Issue 7
DOI: 10.1073/pnas.0608582104

Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles
journal, January 2006

Owensiii, D.; Peppas, N.
International Journal of Pharmaceutics, Vol. 307, Issue 1
DOI: 10.1016/j.ijpharm.2005.10.010

‘Stealth’ corona-core nanoparticles surface modified by polyethylene glycol (PEG): influences of the corona (PEG chain length and surface density) and of the core composition on phagocytic uptake and plasma protein adsorption
journal, October 2000

Gref, R.; Lück, M.; Quellec, P.
Colloids and Surfaces B: Biointerfaces, Vol. 18, Issue 3-4
DOI: 10.1016/S0927-7765(99)00156-3

Multidentate Zwitterionic Ligands Provide Compact and Highly Biocompatible Quantum Dots
journal, September 2013

Zhan, Naiqian; Palui, Goutam; Safi, Malak
Journal of the American Chemical Society, Vol. 135, Issue 37
DOI: 10.1021/ja405010v

Combining Ligand Design with Photoligation to Provide Compact, Colloidally Stable, and Easy to Conjugate Quantum Dots
journal, January 2013

Zhan, Naiqian; Palui, Goutam; Grise, Henry
ACS Applied Materials & Interfaces, Vol. 5, Issue 8
DOI: 10.1021/am302788q

One-Pot, Exchange-Free, Room-Temperature Synthesis of Sub-10 nm Aqueous, Noninteracting, and Stable Zwitterated Iron Oxide Nanoparticles
journal, February 2013

Estephan, Zaki G.; Hariri, Hanaa H.; Schlenoff, Joseph B.
Langmuir, Vol. 29, Issue 8
DOI: 10.1021/la304872d

Zwitterion-Stabilized Silica Nanoparticles: Toward Nonstick Nano
journal, November 2010

Estephan, Zaki G.; Jaber, Jad A.; Schlenoff, Joseph B.
Langmuir, Vol. 26, Issue 22
DOI: 10.1021/la103095d

Fabrication of Corona-Free Nanoparticles with Tunable Hydrophobicity
journal, June 2014

Moyano, Daniel F.; Saha, Krishnendu; Prakash, Gyan
ACS Nano, Vol. 8, Issue 7
DOI: 10.1021/nn5006478

Reverse Microemulsion-Mediated Synthesis of Silica-Coated Gold and Silver Nanoparticles
journal, June 2008

Han, Yu; Jiang, Jiang; Lee, Su Seong
Langmuir, Vol. 24, Issue 11
DOI: 10.1021/la703440p

Synthesis of Silica-Coated Semiconductor and Magnetic Quantum Dots and Their Use in the Imaging of Live Cells
journal, March 2007

Selvan, S. Tamil; Patra, Pranab K.; Ang, Chung Yen
Angewandte Chemie International Edition, Vol. 46, Issue 14
DOI: 10.1002/anie.200604245

Single Quantum Dot-Micelles Coated with Silica Shell as Potentially Non-Cytotoxic Fluorescent Cell Tracers
journal, April 2006

Zhelev, Zhivko; Ohba, Hideki; Bakalova, Rumiana
Journal of the American Chemical Society, Vol. 128, Issue 19
DOI: 10.1021/ja061137d

Synthesis and Properties of Biocompatible Water-Soluble Silica-Coated CdSe/ZnS Semiconductor Quantum Dots ^†
journal, September 2001

Gerion, Daniele; Pinaud, Fabien; Williams, Shara C.
The Journal of Physical Chemistry B, Vol. 105, Issue 37
DOI: 10.1021/jp0105488

Gold Nanoparticle−Cytochrome c Complexes: The Effect of Nanoparticle Ligand Charge on Protein Structure
journal, December 2005

Aubin-Tam, Marie-Eve; Hamad-Schifferli, Kimberly
Langmuir, Vol. 21, Issue 26
DOI: 10.1021/la052102e

Bioactivation and Cell Targeting of Semiconductor CdSe/ZnS Nanocrystals with Phytochelatin-Related Peptides
journal, May 2004

Pinaud, Fabien; King, David; Moore, Hsiao-Ping
Journal of the American Chemical Society, Vol. 126, Issue 19
DOI: 10.1021/ja031691c

Hydrodynamic Dimensions, Electrophoretic Mobility, and Stability of Hydrophilic Quantum Dots
journal, October 2006

Pons, Thomas; Uyeda, H. Tetsuo; Medintz, Igor L.
The Journal of Physical Chemistry B, Vol. 110, Issue 41
DOI: 10.1021/jp065041h

Monovalent, reduced-size quantum dots for imaging receptors on living cells
journal, April 2008

Howarth, Mark; Liu, Wenhao; Puthenveetil, Sujiet
Nature Methods, Vol. 5, Issue 5
DOI: 10.1038/nmeth.1206

Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
journal, September 2013

Tenzer, Stefan; Docter, Dominic; Kuharev, Jörg
Nature Nanotechnology, Vol. 8, Issue 10
DOI: 10.1038/nnano.2013.181

Protein corona significantly reduces active targeting yield
journal, January 2013

Mirshafiee, Vahid; Mahmoudi, Morteza; Lou, Kaiyan
Chemical Communications, Vol. 49, Issue 25
DOI: 10.1039/c3cc37307j

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells
journal, June 2012

Lesniak, Anna; Fenaroli, Federico; Monopoli, Marco P.
ACS Nano, Vol. 6, Issue 7
DOI: 10.1021/nn300223w

Protein Corona Fingerprinting Predicts the Cellular Interaction of Gold and Silver Nanoparticles
journal, February 2014

Walkey, Carl D.; Olsen, Jonathan B.; Song, Fayi
ACS Nano, Vol. 8, Issue 3
DOI: 10.1021/nn406018q

New Insights into the Complexities of Shell Growth and the Strong Influence of Particle Volume in Nonblinking “Giant” Core/Shell Nanocrystal Quantum Dots
journal, May 2012

Ghosh, Yagnaseni; Mangum, Benjamin D.; Casson, Joanna L.
Journal of the American Chemical Society, Vol. 134, Issue 23
DOI: 10.1021/ja212032q

“Giant” Multishell CdSe Nanocrystal Quantum Dots with Suppressed Blinking
journal, April 2008

Chen, Yongfen; Vela, Javier; Htoon, Han
Journal of the American Chemical Society, Vol. 130, Issue 15
DOI: 10.1021/ja711379k

Multifunctional ligands based on dihydrolipoic acid and polyethylene glycol to promote biocompatibility of quantum dots
journal, March 2009

Susumu, Kimihiro; Mei, Bing C.; Mattoussi, Hedi
Nature Protocols, Vol. 4, Issue 3
DOI: 10.1038/nprot.2008.247

Capping of CdSe–ZnS quantum dots with DHLA and subsequent conjugation with proteins
journal, August 2006

Clapp, Aaron R.; Goldman, Ellen R.; Mattoussi, Hedi
Nature Protocols, Vol. 1, Issue 3
DOI: 10.1038/nprot.2006.184

Physical−Chemical Aspects of Protein Corona: Relevance to in Vitro and in Vivo Biological Impacts of Nanoparticles
journal, March 2011

Monopoli, Marco P.; Walczyk, Dorota; Campbell, Abigail
Journal of the American Chemical Society, Vol. 133, Issue 8
DOI: 10.1021/ja107583h

Nanoparticle Size Is a Critical Physicochemical Determinant of the Human Blood Plasma Corona: A Comprehensive Quantitative Proteomic Analysis
journal, August 2011

Tenzer, Stefan; Docter, Dominic; Rosfa, Susanne
ACS Nano, Vol. 5, Issue 9
DOI: 10.1021/nn201950e

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface
journal, January 2013

Salvati, Anna; Pitek, Andrzej S.; Monopoli, Marco P.
Nature Nanotechnology, Vol. 8, Issue 2
DOI: 10.1038/nnano.2012.237

Finishing the euchromatic sequence of the human genome
journal, October 2004

,
Nature, Vol. 431, Issue 7011, p. 931-945
DOI: 10.1038/nature03001

Internalization of HIV-1 Tat Requires Cell Surface Heparan Sulfate Proteoglycans
journal, October 2000

Tyagi, Mudit; Rusnati, Marco; Presta, Marco
Journal of Biological Chemistry, Vol. 276, Issue 5
DOI: 10.1074/jbc.M006701200

A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus
journal, June 1997

Vivès, Eric; Brodin, Priscille; Lebleu, Bernard
Journal of Biological Chemistry, Vol. 272, Issue 25
DOI: 10.1074/jbc.272.25.16010

Untangling the ErbB signalling network
journal, February 2001

Yarden, Yosef; Sliwkowski, Mark X.
Nature Reviews Molecular Cell Biology, Vol. 2, Issue 2
DOI: 10.1038/35052073

Guiding a confocal microscope by single fluorescent nanoparticles
journal, January 2007

Cang, Hu; Xu, C. Shan; Montiel, Daniel
Optics Letters, Vol. 32, Issue 18
DOI: 10.1364/OL.32.002729

Confocal three dimensional tracking of a single nanoparticle with concurrent spectroscopic readouts
journal, May 2006

Cang, Hu; Wong, Chung M.; Xu, C. Shan
Applied Physics Letters, Vol. 88, Issue 22
DOI: 10.1063/1.2204652

Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles
journal, February 2014

Welsher, Kevin; Yang, Haw
Nature Nanotechnology, Vol. 9, Issue 3
DOI: 10.1038/nnano.2014.12

The impact of size on tissue distribution and elimination by single intravenous injection of silica nanoparticles
journal, September 2009

Cho, Minjung; Cho, Wan-Seob; Choi, Mina
Toxicology Letters, Vol. 189, Issue 3
DOI: 10.1016/j.toxlet.2009.04.017

Biodistribution and toxicity of intravenously administered silica nanoparticles in mice
journal, November 2009

Xie, Guangping; Sun, Jiao; Zhong, Gaoren
Archives of Toxicology, Vol. 84, Issue 3
DOI: 10.1007/s00204-009-0488-x

Works referencing / citing this record:

Profiling of nanoparticle–protein interactions by electrophoresis techniques
journal, October 2018

Zarei, Mohammad; Aalaie, Jamal
Analytical and Bioanalytical Chemistry, Vol. 411, Issue 1
DOI: 10.1007/s00216-018-1401-3

Viral nanoparticles can elude protein barriers: exploiting rather than imitating nature
journal, January 2019

Berardi, Alberto; Baldelli Bombelli, Francesca; Thuenemann, Eva C.
Nanoscale, Vol. 11, Issue 5
DOI: 10.1039/c8nr09067j

Robust real-time 3D single-particle tracking using a dynamically moving laser spot
journal, January 2017

Hou, Shangguo; Lang, Xiaoqi; Welsher, Kevin
Optics Letters, Vol. 42, Issue 12
DOI: 10.1364/ol.42.002390

Mixed‐charge bionanointerfaces: Opposite charges work in harmony to meet the challenges in biomedical applications
journal, May 2020

Li, Huan; Li, Xu; Ji, Jian
WIREs Nanomedicine and Nanobiotechnology, Vol. 12, Issue 3
DOI: 10.1002/wnan.1600

Similar Records in DOE PAGES and OSTI.GOV collections:

Subpopulations of liver coated vesicles resolved by preparative agarose gel electrophoresis

Journal Article Kedersha, N L ; Hill, D F ; Kronquist, K E ; ... - J. Cell Biol.; (United States)

Rat liver clathrin coated vesicles (CVs) were separated into several distinct subpopulations using non-sieving concentrations of agarose, which allowed the separation of species differing primarily in surface charge. Using preparative agarose electrophoresis, the CVs were recovered and analyzed for differences in morphology, coat protein composition, and stripped vesicle protein composition. Coat proteins from difference populations appeared identical on SDS PAGE, and triskelions stripped from the different populations showed the same mobility on the agarose gel, suggesting that the mobility differences observed in intact CVs were due to differences in the surface charge of underlying vesicles rather than to variations inmore »« less
https://doi.org/10.1083/jcb.103.1.287
On-line detection of proteins in gel electrophoresis by ultraviolet absorption and by native fluorescence utilizing a charge-coupled device imaging system

Journal Article Koutny, L B ; Yeung, E S - Analytical Chemistry (Washington); (United States)

Slab-gel electrophoresis is the most common technique for the separation of high molecular weight biomolecules such a proteins. Acrylamide gels, as described by Laemmli, are generally the matrix of choice for the separation of SDS-denatured proteins via electrophoresis. Agarose gels, similar to those used for nucleic acids, are also useful for the separation of proteins but have not been widely applied. Agarose gels are advantageous for many reasons including simplicity of gel casting, easy sample recovery, and the fact that it is nontoxic to both the experimenter and the proteins. In the past, agarose was not used because of itsmore »« less
https://doi.org/10.1021/ac00050a017
Electric birefrigence imaging of DNA in agarose electrophoresis gels

Miscellaneous Lanan, M

Electric birefringence imaging (EBI) provides sensitive, non-invasive detection of double-stranded DNA in agarose gels. Quasi-monochromatic, visible light is transmitted through an electrophoresis gel which is placed between plastic film polarizers. A slow-scan video camera equipped with a 12 bit A/D converter records the images. Under electrophoresis running conditions, hydrodynamically-induced gel distortion is shown to be the major source of birefringence for fragments smaller than 23 kbp. The birefringence generated approximates the DNA concentration gradient in the electric field direction. The stress-optic coefficient of 1% agarose gel is measured by mechanical compression and used to evaluate the magnitude of the inducedmore »« less
Measurement of DNA damage using agarose gel electrophoresis and electronic imaging

Conference Sutherland, J C ; Bergman, A M ; Chen, Chun-Zhang ; ...

Damage done to DNA by ultraviolet (uv) light, gamma rays and other carcinogens can be quantified using agarose gel electrophororesis. Agents that either produce strand breaks directly or that produce lesions that can be enzymatically or chemically converted to strand breaks can be studied. The method requires: (1) accurate measurement of the disribution of mass of DNA as a function of the distance of migration in the gel, (2) determination of the dispersion function of the electrophoresis system and (3) calculation of weighted averages of these functions by a computer. Less than 50 ng of DNA are required and themore »« less
Optimization of the southern electrophoretic transfer method

Technical Report Allison, M A ; Fujimura, R K

The technique of separating DNA fragments using agarose gel electrophoresis is essential in the analysis of nucleic acids. Further, after the method of transferring specific DNA fragments from those agarose gels to cellulose nitrate membranes was developed in 1975, a method was developed to transfer DNA, RNA, protein and ribonucleoprotein particles from various gels onto diazobenzyloxymethyl (DBM) paper using electrophoresis as well. This paper describes the optimum conditions for quantitative electrophoretic transfer of DNA onto nylon membranes. This method exemplifies the ability to hybridize the membrane more than once with specific RNA probes by providing sufficient retention of the DNA.more »« less

Similar Records

Title: Discovery of protein- and DNA-imperceptible nanoparticle hard coating using gel-based reaction tuning

Abstract

Citation Formats

Understanding and controlling the interaction of nanomaterials with proteins in a physiological environment journal, January 2012

Biomolecular coronas provide the biological identity of nanosized materials journal, December 2012

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts journal, September 2008

Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles journal, January 2007

Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles journal, January 2006

‘Stealth’ corona-core nanoparticles surface modified by polyethylene glycol (PEG): influences of the corona (PEG chain length and surface density) and of the core composition on phagocytic uptake and plasma protein adsorption journal, October 2000

Multidentate Zwitterionic Ligands Provide Compact and Highly Biocompatible Quantum Dots journal, September 2013

Combining Ligand Design with Photoligation to Provide Compact, Colloidally Stable, and Easy to Conjugate Quantum Dots journal, January 2013

One-Pot, Exchange-Free, Room-Temperature Synthesis of Sub-10 nm Aqueous, Noninteracting, and Stable Zwitterated Iron Oxide Nanoparticles journal, February 2013

Zwitterion-Stabilized Silica Nanoparticles: Toward Nonstick Nano journal, November 2010

Fabrication of Corona-Free Nanoparticles with Tunable Hydrophobicity journal, June 2014

Reverse Microemulsion-Mediated Synthesis of Silica-Coated Gold and Silver Nanoparticles journal, June 2008

Synthesis of Silica-Coated Semiconductor and Magnetic Quantum Dots and Their Use in the Imaging of Live Cells journal, March 2007

Single Quantum Dot-Micelles Coated with Silica Shell as Potentially Non-Cytotoxic Fluorescent Cell Tracers journal, April 2006

Synthesis and Properties of Biocompatible Water-Soluble Silica-Coated CdSe/ZnS Semiconductor Quantum Dots † journal, September 2001

Gold Nanoparticle−Cytochrome c Complexes: The Effect of Nanoparticle Ligand Charge on Protein Structure journal, December 2005

Bioactivation and Cell Targeting of Semiconductor CdSe/ZnS Nanocrystals with Phytochelatin-Related Peptides journal, May 2004

Hydrodynamic Dimensions, Electrophoretic Mobility, and Stability of Hydrophilic Quantum Dots journal, October 2006

Monovalent, reduced-size quantum dots for imaging receptors on living cells journal, April 2008

Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology journal, September 2013

Protein corona significantly reduces active targeting yield journal, January 2013

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells journal, June 2012

Protein Corona Fingerprinting Predicts the Cellular Interaction of Gold and Silver Nanoparticles journal, February 2014

New Insights into the Complexities of Shell Growth and the Strong Influence of Particle Volume in Nonblinking “Giant” Core/Shell Nanocrystal Quantum Dots journal, May 2012

“Giant” Multishell CdSe Nanocrystal Quantum Dots with Suppressed Blinking journal, April 2008

Multifunctional ligands based on dihydrolipoic acid and polyethylene glycol to promote biocompatibility of quantum dots journal, March 2009

Capping of CdSe–ZnS quantum dots with DHLA and subsequent conjugation with proteins journal, August 2006

Physical−Chemical Aspects of Protein Corona: Relevance to in Vitro and in Vivo Biological Impacts of Nanoparticles journal, March 2011

Nanoparticle Size Is a Critical Physicochemical Determinant of the Human Blood Plasma Corona: A Comprehensive Quantitative Proteomic Analysis journal, August 2011

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface journal, January 2013

Finishing the euchromatic sequence of the human genome journal, October 2004

Internalization of HIV-1 Tat Requires Cell Surface Heparan Sulfate Proteoglycans journal, October 2000

A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus journal, June 1997

Untangling the ErbB signalling network journal, February 2001

Guiding a confocal microscope by single fluorescent nanoparticles journal, January 2007

Confocal three dimensional tracking of a single nanoparticle with concurrent spectroscopic readouts journal, May 2006

Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles journal, February 2014

The impact of size on tissue distribution and elimination by single intravenous injection of silica nanoparticles journal, September 2009

Biodistribution and toxicity of intravenously administered silica nanoparticles in mice journal, November 2009

Profiling of nanoparticle–protein interactions by electrophoresis techniques journal, October 2018

Viral nanoparticles can elude protein barriers: exploiting rather than imitating nature journal, January 2019

Robust real-time 3D single-particle tracking using a dynamically moving laser spot journal, January 2017

Mixed‐charge bionanointerfaces: Opposite charges work in harmony to meet the challenges in biomedical applications journal, May 2020

Understanding and controlling the interaction of nanomaterials with proteins in a physiological environment
journal, January 2012

Biomolecular coronas provide the biological identity of nanosized materials
journal, December 2012

Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts
journal, September 2008

Understanding the nanoparticle-protein corona using methods to quantify exchange rates and affinities of proteins for nanoparticles
journal, January 2007

Opsonization, biodistribution, and pharmacokinetics of polymeric nanoparticles
journal, January 2006

‘Stealth’ corona-core nanoparticles surface modified by polyethylene glycol (PEG): influences of the corona (PEG chain length and surface density) and of the core composition on phagocytic uptake and plasma protein adsorption
journal, October 2000

Multidentate Zwitterionic Ligands Provide Compact and Highly Biocompatible Quantum Dots
journal, September 2013

Combining Ligand Design with Photoligation to Provide Compact, Colloidally Stable, and Easy to Conjugate Quantum Dots
journal, January 2013

One-Pot, Exchange-Free, Room-Temperature Synthesis of Sub-10 nm Aqueous, Noninteracting, and Stable Zwitterated Iron Oxide Nanoparticles
journal, February 2013

Zwitterion-Stabilized Silica Nanoparticles: Toward Nonstick Nano
journal, November 2010

Fabrication of Corona-Free Nanoparticles with Tunable Hydrophobicity
journal, June 2014

Reverse Microemulsion-Mediated Synthesis of Silica-Coated Gold and Silver Nanoparticles
journal, June 2008

Synthesis of Silica-Coated Semiconductor and Magnetic Quantum Dots and Their Use in the Imaging of Live Cells
journal, March 2007

Single Quantum Dot-Micelles Coated with Silica Shell as Potentially Non-Cytotoxic Fluorescent Cell Tracers
journal, April 2006

Synthesis and Properties of Biocompatible Water-Soluble Silica-Coated CdSe/ZnS Semiconductor Quantum Dots ^†
journal, September 2001

Gold Nanoparticle−Cytochrome c Complexes: The Effect of Nanoparticle Ligand Charge on Protein Structure
journal, December 2005

Bioactivation and Cell Targeting of Semiconductor CdSe/ZnS Nanocrystals with Phytochelatin-Related Peptides
journal, May 2004

Hydrodynamic Dimensions, Electrophoretic Mobility, and Stability of Hydrophilic Quantum Dots
journal, October 2006

Monovalent, reduced-size quantum dots for imaging receptors on living cells
journal, April 2008

Rapid formation of plasma protein corona critically affects nanoparticle pathophysiology
journal, September 2013

Protein corona significantly reduces active targeting yield
journal, January 2013

Effects of the Presence or Absence of a Protein Corona on Silica Nanoparticle Uptake and Impact on Cells
journal, June 2012

Protein Corona Fingerprinting Predicts the Cellular Interaction of Gold and Silver Nanoparticles
journal, February 2014

New Insights into the Complexities of Shell Growth and the Strong Influence of Particle Volume in Nonblinking “Giant” Core/Shell Nanocrystal Quantum Dots
journal, May 2012

“Giant” Multishell CdSe Nanocrystal Quantum Dots with Suppressed Blinking
journal, April 2008

Multifunctional ligands based on dihydrolipoic acid and polyethylene glycol to promote biocompatibility of quantum dots
journal, March 2009

Capping of CdSe–ZnS quantum dots with DHLA and subsequent conjugation with proteins
journal, August 2006

Physical−Chemical Aspects of Protein Corona: Relevance to in Vitro and in Vivo Biological Impacts of Nanoparticles
journal, March 2011

Nanoparticle Size Is a Critical Physicochemical Determinant of the Human Blood Plasma Corona: A Comprehensive Quantitative Proteomic Analysis
journal, August 2011

Transferrin-functionalized nanoparticles lose their targeting capabilities when a biomolecule corona adsorbs on the surface
journal, January 2013

Finishing the euchromatic sequence of the human genome
journal, October 2004

Internalization of HIV-1 Tat Requires Cell Surface Heparan Sulfate Proteoglycans
journal, October 2000

A Truncated HIV-1 Tat Protein Basic Domain Rapidly Translocates through the Plasma Membrane and Accumulates in the Cell Nucleus
journal, June 1997

Untangling the ErbB signalling network
journal, February 2001

Guiding a confocal microscope by single fluorescent nanoparticles
journal, January 2007

Confocal three dimensional tracking of a single nanoparticle with concurrent spectroscopic readouts
journal, May 2006

Multi-resolution 3D visualization of the early stages of cellular uptake of peptide-coated nanoparticles
journal, February 2014

The impact of size on tissue distribution and elimination by single intravenous injection of silica nanoparticles
journal, September 2009

Biodistribution and toxicity of intravenously administered silica nanoparticles in mice
journal, November 2009

Profiling of nanoparticle–protein interactions by electrophoresis techniques
journal, October 2018

Viral nanoparticles can elude protein barriers: exploiting rather than imitating nature
journal, January 2019

Robust real-time 3D single-particle tracking using a dynamically moving laser spot
journal, January 2017

Mixed‐charge bionanointerfaces: Opposite charges work in harmony to meet the challenges in biomedical applications
journal, May 2020