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Title: Specificity and Heterogeneity of Terahertz Radiation Effect on Gene Expression in Mouse Mesenchymal Stem Cells

Abstract

In this paper, we report that terahertz (THz) irradiation of mouse mesenchymal stem cells (mMSCs) with a single-frequency (SF) 2.52 THz laser or pulsed broadband (centered at 10 THz) source results in irradiation specific heterogenic changes in gene expression. The THz effect depends on irradiation parameters such as the duration and type of THz source, and on the degree of stem cell differentiation. Our microarray survey and RT-PCR experiments demonstrate that prolonged broadband THz irradiation drives mMSCs toward differentiation, while 2-hour irradiation (regardless of THz sources) affects genes transcriptionally active in pluripotent stem cells. The strictly controlled experimental environment indicates minimal temperature changes and the absence of any discernable response to heat shock and cellular stress genes imply a non-thermal response. Computer simulations of the core promoters of two pluripotency markers reveal association between gene upregulation and propensity for DNA breathing. Finally, we propose that THz radiation has potential for non-contact control of cellular gene expression.

Authors:
 [1];  [2];  [3];  [4];  [5];  [6];  [4];  [2];  [2];  [7];  [2];  [7];  [6]
  1. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Theoretical Division; Harvard Medical School, Boston, MA (United States). Beth Israel Deaconess Medical Center. Dept. of Medicine
  2. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Center for Integrated Nanotechnologies
  3. Wellcome Trust Sanger Inst., Cambridge (United Kingdom). Cancer Genome Project
  4. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Materials Physics and Applications Division. National High Magnetic Field Lab. (NHMFL)
  5. Harvard Medical School, Boston, MA (United States). Beth Israel Deaconess Medical Center. Dept. of Medicine; Univ. Hospital Zurich (Switzerland). Research Unit of the Division of Internal Medicine
  6. Harvard Medical School, Boston, MA (United States). Beth Israel Deaconess Medical Center. Dept. of Medicine
  7. Los Alamos National Lab. (LANL), Los Alamos, NM (United States). Theoretical Division
Publication Date:
Research Org.:
Los Alamos National Lab. (LANL), Los Alamos, NM (United States); Harvard Medical School, Boston, MA (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)
OSTI Identifier:
1321721
Report Number(s):
LA-UR-12-24696
Journal ID: ISSN 2045-2322
Grant/Contract Number:  
AC52-06NA25396; AC04-94AL85000; 20110516ECR
Resource Type:
Accepted Manuscript
Journal Name:
Scientific Reports
Additional Journal Information:
Journal Volume: 3; Journal ID: ISSN 2045-2322
Publisher:
Nature Publishing Group
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; applied optics; computational biophysics; mesenchymal stem cells; transcription

Citation Formats

Alexandrov, Boian S., Phipps, M. Lisa, Alexandrov, Ludmil B., Booshehri, Layla G., Erat, Anna, Zabolotny, Janice, Mielke, Charles H., Chen, Hou-Tong, Rodriguez, George, Rasmussen, Kim O., Martinez, Jennifer S., Bishop, Alan R., and Usheva, Anny. Specificity and Heterogeneity of Terahertz Radiation Effect on Gene Expression in Mouse Mesenchymal Stem Cells. United States: N. p., 2013. Web. doi:10.1038/srep01184.
Alexandrov, Boian S., Phipps, M. Lisa, Alexandrov, Ludmil B., Booshehri, Layla G., Erat, Anna, Zabolotny, Janice, Mielke, Charles H., Chen, Hou-Tong, Rodriguez, George, Rasmussen, Kim O., Martinez, Jennifer S., Bishop, Alan R., & Usheva, Anny. Specificity and Heterogeneity of Terahertz Radiation Effect on Gene Expression in Mouse Mesenchymal Stem Cells. United States. doi:10.1038/srep01184.
Alexandrov, Boian S., Phipps, M. Lisa, Alexandrov, Ludmil B., Booshehri, Layla G., Erat, Anna, Zabolotny, Janice, Mielke, Charles H., Chen, Hou-Tong, Rodriguez, George, Rasmussen, Kim O., Martinez, Jennifer S., Bishop, Alan R., and Usheva, Anny. Thu . "Specificity and Heterogeneity of Terahertz Radiation Effect on Gene Expression in Mouse Mesenchymal Stem Cells". United States. doi:10.1038/srep01184. https://www.osti.gov/servlets/purl/1321721.
@article{osti_1321721,
title = {Specificity and Heterogeneity of Terahertz Radiation Effect on Gene Expression in Mouse Mesenchymal Stem Cells},
author = {Alexandrov, Boian S. and Phipps, M. Lisa and Alexandrov, Ludmil B. and Booshehri, Layla G. and Erat, Anna and Zabolotny, Janice and Mielke, Charles H. and Chen, Hou-Tong and Rodriguez, George and Rasmussen, Kim O. and Martinez, Jennifer S. and Bishop, Alan R. and Usheva, Anny},
abstractNote = {In this paper, we report that terahertz (THz) irradiation of mouse mesenchymal stem cells (mMSCs) with a single-frequency (SF) 2.52 THz laser or pulsed broadband (centered at 10 THz) source results in irradiation specific heterogenic changes in gene expression. The THz effect depends on irradiation parameters such as the duration and type of THz source, and on the degree of stem cell differentiation. Our microarray survey and RT-PCR experiments demonstrate that prolonged broadband THz irradiation drives mMSCs toward differentiation, while 2-hour irradiation (regardless of THz sources) affects genes transcriptionally active in pluripotent stem cells. The strictly controlled experimental environment indicates minimal temperature changes and the absence of any discernable response to heat shock and cellular stress genes imply a non-thermal response. Computer simulations of the core promoters of two pluripotency markers reveal association between gene upregulation and propensity for DNA breathing. Finally, we propose that THz radiation has potential for non-contact control of cellular gene expression.},
doi = {10.1038/srep01184},
journal = {Scientific Reports},
number = ,
volume = 3,
place = {United States},
year = {2013},
month = {1}
}

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