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Title: Quantification of Lysine Acetylation and Succinylation Stoichiometry in Proteins Using Mass Spectrometric Data-Independent Acquisitions (SWATH)

Journal Article · · Journal of the American Society for Mass Spectrometry
 [1];  [1];  [1];  [2];  [3];  [4];  [1]
  1. Buck Institute for Research on Aging, 94945, Novato, CA, USA
  2. Amgen, 94080, South San Francisco, CA, USA
  3. Department of Microbiology and Immunology, Stritch School of Medicine, Health Sciences Division, Loyola University Chicago, 60153, Maywood, IL, USA
  4. Buck Institute for Research on Aging, 94945, Novato, CA, USA, Department of Pharmaceutical Chemistry, University of California, 94143, San Francisco, CA, USA

Not Available

Sponsoring Organization:
USDOE
Grant/Contract Number:
SC0012443
OSTI ID:
1315863
Journal Information:
Journal of the American Society for Mass Spectrometry, Journal Name: Journal of the American Society for Mass Spectrometry Journal Issue: 11 Vol. 27; ISSN 1044-0305
Publisher:
American Chemical SocietyCopyright Statement
Country of Publication:
United States
Language:
English

References (54)

Probability-based protein identification by searching sequence databases using mass spectrometry data journal December 1999
The E. coli sirtuin CobB shows no preference for enzymatic and nonenzymatic lysine acetylation substrate sites journal November 2014
Acetylation dynamics and stoichiometry in S accharomyces cerevisiae journal January 2014
Reversed-phase chromatography with multiple fraction concatenation strategy for proteome profiling of human MCF10A cells journal April 2011
Regulation of acetate metabolism in Escherichia coli BL21 by protein Nε-lysine acetylation journal December 2014
[41] Acetylation book January 1972
Lysine Succinylation Is a Frequently Occurring Modification in Prokaryotes and Eukaryotes and Extensively Overlaps with Acetylation journal August 2013
SIRT5 Regulates the Mitochondrial Lysine Succinylome and Metabolic Networks journal December 2013
Substrate and Functional Diversity of Lysine Acetylation Revealed by a Proteomics Survey journal August 2006
Lysine Acetylation: Codified Crosstalk with Other Posttranslational Modifications journal August 2008
SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways journal June 2013
Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli journal July 2013
SIRT5 Regulates both Cytosolic and Mitochondrial Protein Malonylation with Glycolysis as a Major Target journal July 2015
Mitochondrial sirtuins: regulators of protein acylation and metabolism journal September 2012
Controlling Deuterium Isotope Effects in Comparative Proteomics journal August 2002
Empirical Statistical Model To Estimate the Accuracy of Peptide Identifications Made by MS/MS and Database Search journal October 2002
Multiplexed, Scheduled, High-Resolution Parallel Reaction Monitoring on a Full Scan QqTOF Instrument with Integrated Data-Dependent and Targeted Mass Spectrometric Workflows journal September 2015
Site-Specific Identification of Lysine Acetylation Stoichiometries in Mammalian Cells journal February 2016
Regulation of chromatin by histone modifications journal February 2011
SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation journal March 2010
A cross-platform toolkit for mass spectrometry and proteomics journal October 2012
MS-GF+ makes progress towards a universal database search tool for proteomics journal October 2014
DIA-Umpire: comprehensive computational framework for data-independent acquisition proteomics journal January 2015
The growing landscape of lysine acetylation links metabolism and cell signalling journal July 2014
50 years of protein acetylation: from gene regulation to epigenetics, metabolism and beyond journal December 2014
Histone deacetylases and cancer journal August 2007
Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways journal April 2013
Inhibition of Acetyl Phosphate-dependent Transcription by an Acetylatable Lysine on RNA Polymerase journal July 2012
Quantification of Mitochondrial Acetylation Dynamics Highlights Prominent Sites of Metabolic Regulation journal July 2013
Widespread and Enzyme-independent N ϵ -Acetylation and N ϵ -Succinylation of Proteins in the Chemical Conditions of the Mitochondrial Matrix journal August 2013
Stoichiometry of Site-specific Lysine Acetylation in an Entire Proteome journal June 2014
iProphet: Multi-level Integrative Analysis of Shotgun Proteomic Data Improves Peptide and Protein Identification Rates and Error Estimates journal August 2011
The First Identification of Lysine Malonylation Substrates and Its Regulatory Enzyme journal September 2011
Platform-independent and Label-free Quantitation of Proteomic Data Using MS1 Extracted Ion Chromatograms in Skyline: APPLICATION TO PROTEIN ACETYLATION AND PHOSPHORYLATION journal March 2012
Identification of Lysine Succinylation Substrates and the Succinylation Regulatory Enzyme CobB in Escherichia coli journal October 2013
Proteomic and Biochemical Studies of Lysine Malonylation Suggest Its Malonic Aciduria-associated Regulatory Role in Mitochondrial Function and Fatty Acid Oxidation journal August 2015
Quantitative, Multiplexed Assays for Low Abundance Proteins in Plasma by Targeted Mass Spectrometry and Stable Isotope Dilution journal October 2007
Lysine Acetylation Is a Highly Abundant and Evolutionarily Conserved Modification in Escherichia Coli journal August 2008
Deep, Quantitative Coverage of the Lysine Acetylome Using Novel Anti-acetyl-lysine Antibodies and an Optimized Proteomic Workflow journal May 2015
MS1 Peptide Ion Intensity Chromatograms in MS2 (SWATH) Data Independent Acquisitions. Improving Post Acquisition Analysis of Proteomic Experiments journal May 2015
Metabolic Regulation by Lysine Malonylation, Succinylation, and Glutarylation journal February 2015
The human silent information regulator (Sir)2 homologue hSIRT3 is a mitochondrial nicotinamide adenine dinucleotide–dependent deacetylase journal August 2002
Evolutionarily Conserved and Nonconserved Cellular Localizations and Functions of Human SIRT Proteins journal October 2005
Skyline: an open source document editor for creating and analyzing targeted proteomics experiments journal February 2010
Involvement of protein acetylation in glucose-induced transcription of a stress-responsive promoter: Acetylation of RNA polymerase journal July 2011
Calorie restriction alters mitochondrial protein acetylation journal October 2009
Protein acetylation dynamics in response to carbon overflow in E scherichia coli : Acetylation dynamics and overflow metabolism journal September 2015
Lysine Acetylation Targets Protein Complexes and Co-Regulates Major Cellular Functions journal July 2009
Quantitative Phosphoproteomics Reveals Widespread Full Phosphorylation Site Occupancy During Mitosis journal January 2010
Acetylation of the Response Regulator RcsB Controls Transcription from a Small RNA Promoter journal July 2013
A Method to Determine Lysine Acetylation Stoichiometries journal January 2014
Structural, Kinetic and Proteomic Characterization of Acetyl Phosphate-Dependent Bacterial Protein Acetylation journal April 2014
Analysis of acetylation stoichiometry suggests that SIRT 3 repairs nonenzymatic acetylation lesions journal September 2015
Protein acetylation affects acetate metabolism, motility and acid stress response in Escherichia coli journal November 2014

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