The role of membrane fluidization in the gel-assisted formation of giant polymersomes
Abstract
Polymersomes are being widely explored as synthetic analogs of lipid vesicles based on their enhanced stability and potential uses in a wide variety of applications in (e.g., drug delivery, cell analogs, etc.). Controlled formation of giant polymersomes for use in membrane studies and cell mimetic systems, however, is currently limited by low-yield production methodologies. Here, we describe for the first time, how the size distribution of giant poly(ethylene glycol)-poly(butadiene) (PEO-PBD) polymersomes formed by gel-assisted rehydration may be controlled based on membrane fluidization. We first show that the average diameter and size distribution of PEO-PBD polymersomes may be readily increased by increasing the temperature of the rehydration solution. Further, we describe a correlative relationship between polymersome size and membrane fluidization through the addition of sucrose during rehydration, enabling the formation of PEO-PBD polymersomes with a range of diameters, including giant-sized vesicles (>100 μm). This correlative relationship suggests that sucrose may function as a small molecule fluidizer during rehydration, enhancing polymer diffusivity during formation and increasing polymersome size. Altogether the ability to easily regulate the size of PEO-PBD polymersomes based on membrane fluidity, either through temperature or fluidizers, has broadly applicability in areas including targeted therapeutic delivery and synthetic biology.
- Authors:
-
[1];
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Martin-Luther-Univ., Halle-Wittenberg (Germany)
- Publication Date:
- Research Org.:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1291338
- Report Number(s):
- SAND-2016-7533J
Journal ID: ISSN 1932-6203; 646370
- Grant/Contract Number:
- AC04-94AL85000
- Resource Type:
- Accepted Manuscript
- Journal Name:
- PLoS ONE
- Additional Journal Information:
- Journal Volume: 11; Journal Issue: 7; Journal ID: ISSN 1932-6203
- Publisher:
- Public Library of Science
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 36 MATERIALS SCIENCE; polymers; vesicles; fluorescence recovery after photobleaching; sucrose; lipids; gel electrophoresis; mass diffusivity; membrane potential
Citation Formats
Greene, Adrienne C., Henderson, Ian M., Gomez, Andrew, Paxton, Walter F., VanDelinder, Virginia, Bachand, George D., and Hinderberger, Dariush. The role of membrane fluidization in the gel-assisted formation of giant polymersomes. United States: N. p., 2016.
Web. doi:10.1371/journal.pone.0158729.
Greene, Adrienne C., Henderson, Ian M., Gomez, Andrew, Paxton, Walter F., VanDelinder, Virginia, Bachand, George D., & Hinderberger, Dariush. The role of membrane fluidization in the gel-assisted formation of giant polymersomes. United States. https://doi.org/10.1371/journal.pone.0158729
Greene, Adrienne C., Henderson, Ian M., Gomez, Andrew, Paxton, Walter F., VanDelinder, Virginia, Bachand, George D., and Hinderberger, Dariush. Wed .
"The role of membrane fluidization in the gel-assisted formation of giant polymersomes". United States. https://doi.org/10.1371/journal.pone.0158729. https://www.osti.gov/servlets/purl/1291338.
@article{osti_1291338,
title = {The role of membrane fluidization in the gel-assisted formation of giant polymersomes},
author = {Greene, Adrienne C. and Henderson, Ian M. and Gomez, Andrew and Paxton, Walter F. and VanDelinder, Virginia and Bachand, George D. and Hinderberger, Dariush},
abstractNote = {Polymersomes are being widely explored as synthetic analogs of lipid vesicles based on their enhanced stability and potential uses in a wide variety of applications in (e.g., drug delivery, cell analogs, etc.). Controlled formation of giant polymersomes for use in membrane studies and cell mimetic systems, however, is currently limited by low-yield production methodologies. Here, we describe for the first time, how the size distribution of giant poly(ethylene glycol)-poly(butadiene) (PEO-PBD) polymersomes formed by gel-assisted rehydration may be controlled based on membrane fluidization. We first show that the average diameter and size distribution of PEO-PBD polymersomes may be readily increased by increasing the temperature of the rehydration solution. Further, we describe a correlative relationship between polymersome size and membrane fluidization through the addition of sucrose during rehydration, enabling the formation of PEO-PBD polymersomes with a range of diameters, including giant-sized vesicles (>100 μm). This correlative relationship suggests that sucrose may function as a small molecule fluidizer during rehydration, enhancing polymer diffusivity during formation and increasing polymersome size. Altogether the ability to easily regulate the size of PEO-PBD polymersomes based on membrane fluidity, either through temperature or fluidizers, has broadly applicability in areas including targeted therapeutic delivery and synthetic biology.},
doi = {10.1371/journal.pone.0158729},
journal = {PLoS ONE},
number = 7,
volume = 11,
place = {United States},
year = {Wed Jul 13 00:00:00 EDT 2016},
month = {Wed Jul 13 00:00:00 EDT 2016}
}
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Films of Agarose Enable Rapid Formation of Giant Liposomes in Solutions of Physiologic Ionic Strength
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From Membranes to Melts, Rouse to Reptation: Diffusion in Polymersome versus Lipid Bilayers
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Size-Controlled Self-Assembly of Superparamagnetic Polymersomes
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Spotted vesicles, striped micelles and Janus assemblies induced by ligand binding
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Formation and material properties of giant liquid crystal polymersomes
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Tuning polymersome surfaces: functionalization with dendritic groups
journal, January 2012
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Biocompatible and biodegradable polymersomes as delivery vehicles in biomedical applications
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Polymersomes: Tough Vesicles Made from Diblock Copolymers
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Hyperviscous diblock copolymer vesicles
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Giant Host Red Blood Cell Membrane Mimicking Polymersomes Bind Parasite Proteins and Malaria Parasites
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Asymmetric Lipid Membranes: Towards More Realistic Model Systems
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