Single‐Walled Carbon Nanotubes as Fluorescence Biosensors for Pathogen Recognition in Water Systems

Upadhyayula, Venkata K. K.; Ghoshroy, Soumitra; Nair, Vinod S.; Smith, Geoffrey B.; Mitchell, Martha C.; Deng, Shuguang; Gupta, ed., Ram B.

doi:10.1155/2008/156358

Title: Single‐Walled Carbon Nanotubes as Fluorescence Biosensors for Pathogen Recognition in Water Systems

Journal Article · Thu Jun 19 00:00:00 EDT 2008 · Journal of Nanotechnology

DOI: https://doi.org/10.1155/2008/156358 · OSTI ID:1290298

Upadhyayula, Venkata K. K.; Ghoshroy, Soumitra; Nair, Vinod S.; Smith, Geoffrey B.; Mitchell, Martha C.; Deng, Shuguang; Gupta, ed., Ram B.

The possibility of using single‐walled carbon nanotubes (SWCNTs) aggregates as fluorescence sensors for pathogen recognition in drinking water treatment applications has been studied. Batch adsorption study is conducted to adsorb large concentrations of Staphylococcus aureus aureus SH 1000 and Escherichia coli pKV‐11 on single‐walled carbon nanotubes. Subsequently the immobilized bacteria are detected with confocal microscopy by coating the nanotubes with fluorescence emitting antibodies. The Freundlich adsorption equilibrium constant ( k ) for S.aureus and E.coli determined from batch adsorption study was found to be 9 × 10 ⁸ and 2 × 10 ⁸ ml/g, respectively. The visualization of bacterial cells adsorbed on fluorescently modified carbon nanotubes is also clearly seen. The results indicate that hydrophobic single‐walled carbon nanotubes have excellent bacterial adsorption capacity and fluorescent detection capability. This is an important advancement in designing fluorescence biosensors for pathogen recognition in water systems.

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Sponsoring Organization:: USDOE

OSTI ID:: 1290298

Journal Information:: Journal of Nanotechnology, Journal Name: Journal of Nanotechnology Journal Issue: 1 Vol. 2008; ISSN 1687-9503

Publisher:: Hindawi Publishing CorporationCopyright Statement

Country of Publication:: United Kingdom

Language:: English

References (10)

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