Size-separation of silver nanoparticles using sucrose gradient centrifugation

Suresh, Anil K.; Pelletier, Dale A.; Moon, Ji Won; Phelps, Tommy; Doktycz, Mitchel John

doi:10.4172/2157-7064.1000283

Title: Size-separation of silver nanoparticles using sucrose gradient centrifugation

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Abstract

Size and shape distributions of nanoparticles can drastically contribute to the overall properties of nanoparticles, thereby influencing their interaction with different chemotherapeutic molecules, biological organisms and or materials and cell types. Therefore, to exploit the proper use of nanoparticles for various biomedical and biosensor applications, it is important to obtain well-separated monodispersed nanoparticles. However, gaining precise control over the morphological characteristics of nanoparticles during their synthesis is often a challenging task. Consequently, post-synthesis separation of nanoparticles is necessary. In the present study, we demonstrate the successful one-pot post-synthesis separation of anisotropic silver nanoparticles to near modispersities using sucrose density gradient sedimentation. The separation of the nanoparticles was evidenced based on optical confirmation, and spectrophotometric and transmission electron microscopy measurements. Our results clearly demonstrate the facile separation of anisotropic silver nanoparticles using sucrose density gradient sedimentation and can enable the use of nanoparticles for various biomedical applications.

Authors:

Suresh, Anil K. ^[1]; Pelletier, Dale A. ^[2]; Moon, Ji Won ^[2]; Phelps, Tommy ^[2]; Doktycz, Mitchel John ^[2]

Pondicherry Univ., Kalapet (India)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Publication Date:: Fri Aug 28 00:00:00 EDT 2015

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE Office of Science (SC)

OSTI Identifier:: 1286891

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Chromatography & Separation Techniques

Additional Journal Information:: Journal Volume: 6; Journal Issue: 5; Journal ID: ISSN 2157-7064

Publisher:: OMICS International

Country of Publication:: United States

Language:: English

Subject:: 77 NANOSCIENCE AND NANOTECHNOLOGY; anisotropic; density gradient; fractionation; sucrose; separation; nanoparticles

Citation Formats


                    Suresh, Anil K., Pelletier, Dale A., Moon, Ji Won, Phelps, Tommy, and Doktycz, Mitchel John. Size-separation of silver nanoparticles using sucrose gradient centrifugation.  United States: N. p., 2015. 
Web.  doi:10.4172/2157-7064.1000283.

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                    Suresh, Anil K., Pelletier, Dale A., Moon, Ji Won, Phelps, Tommy, & Doktycz, Mitchel John. Size-separation of silver nanoparticles using sucrose gradient centrifugation.  United States.  https://doi.org/10.4172/2157-7064.1000283

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                    Suresh, Anil K., Pelletier, Dale A., Moon, Ji Won, Phelps, Tommy, and Doktycz, Mitchel John. Fri .  
"Size-separation of silver nanoparticles using sucrose gradient centrifugation".  United States.  https://doi.org/10.4172/2157-7064.1000283.  https://www.osti.gov/servlets/purl/1286891.

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@article{osti_1286891,

  title        = {Size-separation of silver nanoparticles using sucrose gradient centrifugation},

  author       = {Suresh, Anil K. and Pelletier, Dale A. and Moon, Ji Won and Phelps, Tommy and Doktycz, Mitchel John},

  abstractNote = {Size and shape distributions of nanoparticles can drastically contribute to the overall properties of nanoparticles, thereby influencing their interaction with different chemotherapeutic molecules, biological organisms and or materials and cell types. Therefore, to exploit the proper use of nanoparticles for various biomedical and biosensor applications, it is important to obtain well-separated monodispersed nanoparticles. However, gaining precise control over the morphological characteristics of nanoparticles during their synthesis is often a challenging task. Consequently, post-synthesis separation of nanoparticles is necessary. In the present study, we demonstrate the successful one-pot post-synthesis separation of anisotropic silver nanoparticles to near modispersities using sucrose density gradient sedimentation. The separation of the nanoparticles was evidenced based on optical confirmation, and spectrophotometric and transmission electron microscopy measurements. Our results clearly demonstrate the facile separation of anisotropic silver nanoparticles using sucrose density gradient sedimentation and can enable the use of nanoparticles for various biomedical applications.},

  doi          = {10.4172/2157-7064.1000283},

  journal      = {Journal of Chromatography & Separation Techniques},

  number       = 5,

  volume       = 6,

  place        = {United States},

  year         = {Fri Aug 28 00:00:00 EDT 2015},

  month        = {Fri Aug 28 00:00:00 EDT 2015}

}

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