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Title: Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting

Journal Article · · Lab on a chip (Print)
DOI: https://doi.org/10.1039/c4lc01342e · OSTI ID:1282170
 [1];  [2];  [3];  [4];  [5]
  1. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Gladstone Inst., San Francisco, CA (United States)
  2. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Univ. of of California, Berkeley and University of California, San Francisco, CA (United States). Joint Graduate Group in Bioengineering
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boston Univ., Boston, MA (United States). Dept. of Biomedical Engineering
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  5. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Gladstone Inst., San Francisco, CA (United States); Univ. of of California, Berkeley and University of California, San Francisco, CA (United States). Joint Graduate Group in Bioengineering; Univ. of California, San Francisco, CA (United States). California Inst. for Quantitative Biosciences
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Particle sorting using acoustofluidics has enormous potential but widespread adoption has been limited by complex device designs and low throughput. Here, we report high-throughput separation of particles and T lymphocytes (600 μL min-1) by altering the net sonic velocity to reposition acoustic pressure nodes in a simple two-channel device. Finally, the approach is generalizable to other microfluidic platforms for rapid, high-throughput analysis.

Research Organization:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Organization:
USDOE; National Inst. of Health (NIH)
Grant/Contract Number:
AC52-07NA27344
OSTI ID:
1282170
Journal Information:
Lab on a chip (Print), Journal Name: Lab on a chip (Print) Journal Issue: 4 Vol. 15; ISSN LCAHAM; ISSN 1473-0197
Publisher:
Royal Society of ChemistryCopyright Statement
Country of Publication:
United States
Language:
English

References (13)

Mode-switching: A new technique for electronically varying the agglomeration position in an acoustic particle manipulator journal January 2010
Microfluidic, Label-Free Enrichment of Prostate Cancer Cells in Blood Based on Acoustophoresis journal August 2012
Acoustophoretic Sorting of Viable Mammalian Cells in a Microfluidic Device journal November 2012
An On-Chip, Multichannel Droplet Sorter Using Standing Surface Acoustic Waves journal May 2013
Ultrasonic Separation of Particulate Fluids in Small and Large Scale Systems: A Review journal November 2013
Acoustic focusing with engineered node locations for high-performance microfluidic particle separation journal January 2014
Acoustofluidics 7: The acoustic radiation force on small particles journal January 2012
Acoustofluidics 8: Applications of acoustophoresis in continuous flow microsystems journal January 2012
Transportation of single cell and microbubbles by phase-shift introduced to standing leaky surface acoustic waves journal December 2011
Manipulating particle trajectories with phase-control in surface acoustic wave microfluidics journal December 2011
On-chip manipulation of single microparticles, cells, and organisms using surface acoustic waves journal June 2012
A Study of Ultrasonic Velocity and Absorption in Liquid Mixtures journal March 1948
Velocity of Sound in Glycerol journal January 1954

Cited By (2)

A Microfluidic Platform for Precision Small-volume Sample Processing and Its Use to Size Separate Biological Particles with an Acoustic Microdevice journal November 2015
Tunnel Dielectrophoresis for Tunable, Single-Stream Cell Focusing in Physiological Buffers in High-Speed Microfluidic Flows journal June 2016

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59 BASIC BIOLOGICAL SCIENCES