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Title: Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting

Abstract

Particle sorting using acoustofluidics has enormous potential but widespread adoption has been limited by complex device designs and low throughput. Here, we report high-throughput separation of particles and T lymphocytes (600 μL min-1) by altering the net sonic velocity to reposition acoustic pressure nodes in a simple two-channel device. Finally, the approach is generalizable to other microfluidic platforms for rapid, high-throughput analysis.

Authors:
 [1];  [2];  [3];  [4];  [5]
+ Show Author Affiliations
  1. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Gladstone Inst., San Francisco, CA (United States)
  2. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Univ. of of California, Berkeley and University of California, San Francisco, CA (United States). Joint Graduate Group in Bioengineering
  3. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States); Boston Univ., Boston, MA (United States). Dept. of Biomedical Engineering
  4. Lawrence Livermore National Lab. (LLNL), Livermore, CA (United States)
  5. Univ. of California, San Francisco, CA (United States). Dept. of Biochemistry and Biophysics; Gladstone Inst., San Francisco, CA (United States); Univ. of of California, Berkeley and University of California, San Francisco, CA (United States). Joint Graduate Group in Bioengineering; Univ. of California, San Francisco, CA (United States). California Inst. for Quantitative Biosciences
Publication Date:
Research Org.:
Lawrence Livermore National Laboratory (LLNL), Livermore, CA (United States)
Sponsoring Org.:
USDOE; National Institutes of Health (NIH)
OSTI Identifier:
1282170
Grant/Contract Number:  
AC52-07NA27344; 14-LW-077; 1DP1DE024408-01; 1R21A1109611-01A1
Resource Type:
Accepted Manuscript
Journal Name:
Lab on a chip (Print)
Additional Journal Information:
Journal Name: Lab on a chip (Print); Journal Volume: 15; Journal Issue: 4; Journal ID: ISSN 1473-0197
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Jung, Seung-Yong, Notton, Timothy, Fong, Erika, Shusteff, Maxim, and Weinberger, Leor S. Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting. United States: N. p., 2015. Web. doi:10.1039/c4lc01342e.
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Jung, Seung-Yong, Notton, Timothy, Fong, Erika, Shusteff, Maxim, & Weinberger, Leor S. Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting. United States. https://doi.org/10.1039/c4lc01342e
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Jung, Seung-Yong, Notton, Timothy, Fong, Erika, Shusteff, Maxim, and Weinberger, Leor S. Wed . "Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting". United States. https://doi.org/10.1039/c4lc01342e. https://www.osti.gov/servlets/purl/1282170.
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@article{osti_1282170,
title = {Spatial tuning of acoustofluidic pressure nodes by altering net sonic velocity enables high-throughput, efficient cell sorting},
author = {Jung, Seung-Yong and Notton, Timothy and Fong, Erika and Shusteff, Maxim and Weinberger, Leor S.},
abstractNote = {Particle sorting using acoustofluidics has enormous potential but widespread adoption has been limited by complex device designs and low throughput. Here, we report high-throughput separation of particles and T lymphocytes (600 μL min-1) by altering the net sonic velocity to reposition acoustic pressure nodes in a simple two-channel device. Finally, the approach is generalizable to other microfluidic platforms for rapid, high-throughput analysis.},
doi = {10.1039/c4lc01342e},
journal = {Lab on a chip (Print)},
number = 4,
volume = 15,
place = {United States},
year = {Wed Jan 07 00:00:00 EST 2015},
month = {Wed Jan 07 00:00:00 EST 2015}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1039/c4lc01342e
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Cited by: 5 works
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Works referenced in this record:

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    journal, November 2015

    • Fong, Erika J.; Huang, Chao; Hamilton, Julie
    • Journal of Visualized Experiments, Issue 105
    • DOI: 10.3791/53051
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